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Abstract:

Herpes simplex virus type 1 (HSV-1) induces an ocular chronic immunoinflammatory syndrome named herpetic stromal keratitis that can lead to vision impairment and blindness. We have reported that the synthetic brassinosteroid (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one, designated as 2, is a potent antiviral in vitro and reduces the incidence of murine herpetic stromal keratitis, although it does not exert an antiviral effect in vivo. In the present report, we investigated whether brassinosteroid 2 may play a role in the modulation of the response of epithelial and immune cells to HSV-1 infection. Compound 2 blocked HSV-1-induced activation of NF-κB by inhibiting its translocation to the nucleus of infected corneal and conjunctival cells in vitro, as well as significantly reduced the secretion of TNF-α in infected NHC cells. Conversely, IL-6 production was enhanced by compound 2 after HSV-1 infection in both cell types. The production of these cytokines was considerably reduced in a LPS-stimulated macrophage cell line after treatment with compound 2. In conclusion, brassinosteroid 2 would be playing a modulating effect as an inductor or inhibitor, depending on the cell type involved. The improvement of disease observed in mice could be a balance between both, the immunostimulating and immunosuppressive effects of brassinosteroid 2 in vivo. © 2007 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:The in vitro immunomodulatory activity of a synthetic brassinosteroid analogue would account for the improvement of herpetic stromal keratitis in mice
Autor:Michelini, F.M.; Berra, A.; Alché, L.E.
Filiación:Laboratory of Virology, Department of Biochemistry, School of Science, Pabellon II, Piso 4to., Ciudad Universitaria, 1428 Buenos Aires, Argentina
Laboratory of Ocular Immunology, School of Medicine, University of Buenos Aires, J.E. Uriburu 950, E.P, 1113 Buenos Aires, Argentina
Palabras clave:Brassinosteroid; HSV-1; IL-6; Immunomodulatory activity; Murine herpetic stromal keratitis; NF-κB; TNF-α; 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one; brassinosteroid; immunoglobulin enhancer binding protein; interleukin 6; lipopolysaccharide; tumor necrosis factor alpha; unclassified drug; animal cell; antiviral activity; article; controlled study; cornea cell; cytokine production; cytokine release; herpes simplex keratitis; Herpes simplex virus 1; human; human cell; immunomodulation; immunostimulation; immunosuppressive treatment; macrophage; mouse; nonhuman; Animals; Antiviral Agents; Cell Nucleus; Cells, Cultured; Cholestanones; Epithelial Cells; Epithelium, Corneal; Gene Expression Regulation, Viral; Herpesvirus 1, Human; Humans; Immunologic Factors; Interleukin-6; Keratitis, Herpetic; Macrophages; Mice; NF-kappa B; Steroids; Tumor Necrosis Factor-alpha; Viral Envelope Proteins; Human herpesvirus 1; Murinae; Mus
Año:2008
Volumen:108
Número:1-2
Página de inicio:164
Página de fin:170
DOI: http://dx.doi.org/10.1016/j.jsbmb.2007.10.002
Título revista:Journal of Steroid Biochemistry and Molecular Biology
Título revista abreviado:J. Steroid Biochem. Mol. Biol.
ISSN:09600760
CODEN:JSBBE
CAS:3-bromo-5,22,23-trihydroxystigmastan-6-one; Antiviral Agents; Cholestanones; Immunologic Factors; Interleukin-6; NF-kappa B; Steroids; Tumor Necrosis Factor-alpha; Viral Envelope Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v108_n1-2_p164_Michelini

Referencias:

  • Wagner, E.K., Bloom, D.C., Experimental investigation of herpes simplex virus latency (1997) Clin. Microbiol. Rev., 10 (3), pp. 419-443
  • Carr, D.J.J., Härle, P., Gebhardt, B.M., The immune response to ocular herpes simplex virus type 1 infection (2001) Exp. Biol. Med., 226, pp. 353-366
  • Biswas, P.S., Rouse, B.T., Early events in HSV keratitis-setting the stage for a blinding disease (2005) Microb. Infect., 7 (4), pp. 799-810
  • Malíková, J., Swaczynová, J., Kolář, Z., Strnad, M., Anticancer and antiproliferative activity of natural brassinosteroids (2008) Phytochemistry, 69, pp. 418-426
  • Wu, Y.-D., Lou, Y.-J., Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells (2007) Pharmazie, 62 (5), pp. 392-395
  • Michelini, F.M., Ramírez, J.A., Berra, A., Galagovsky, L.R., Alché, L.E., In vitro and in vivo antiherpetic activity of three new synthetic brassinosteroid analogues (2004) Steroids, 69 (11-12), pp. 713-720
  • Kase, S., Aoki, K., Harada, T., Harada, C., Ohgami, K., Shiratori, K., Nishi, S., Yoshida, K., Activation of nuclear factor-kappa B in the conjunctiva with the epithelial scraping of the mouse cornea and human epidemic keratoconjunctivitis (2004) Br. J. Ophthalmol., 88 (7), pp. 947-949
  • Kurt-Jones, E.A., Chan, M., Zhou, S., Wang, J., Reed, G., Bronson, R., Arnold, M.M., Finberg, R.W., Herpes simplex virus 1 interaction with toll-like receptor 2 contributes to lethal encephalitis (2004) Proc. Natl. Acad. Sci. USA, 101 (5), pp. 1315-1320
  • Santoro, M., Rossi, A., Amici, C., NF-kappa B and virus infection: who controls whom (2003) EMBO J., 22 (11), pp. 2552-2560
  • Barquero, A.A., Michelini, F.M., Alché, L.E., 1-Cinnamoyl-3,11-dihydroxymeliacarpin is a natural bioactive compound with antiviral and nuclear factor-kappa B modulating properties (2006) Biochem. Biophys. Res. Commun., 344 (3), pp. 955-962
  • Diebold, Y., Calonge, M., Enríquez de Salamanca, A., Callejo, S., Corrales, R., Sáez, V., Siemasko, K.F., Stern, M.E., Characterization of a spontaneously immortalized cell line (IOBA-NHC) from normal human conjunctiva (2003) Invest. Ophthalmol. Vis. Sci., 44 (10), pp. 4263-4274
  • Denizot, F., Lang, R., Rapid colorimetric assay for cell growth and survival (1986) J. Immunol. Methods, 89, pp. 271-277
  • Decker, T., Lohmann-Mathess, M.L., Gifford, G.E., Cell-associated tumor necrosis factor (TNF) as a killing mechanism of activated cytotoxic macrophages (1987) J. Immunol., 138 (3), pp. 957-962
  • Hiscott, J., Kwon, H., Genin, P., Hostile takeovers: viral appropriation of the NF-kappaB pathway (2001) J. Clin. Invest., 107 (2), pp. 143-151
  • Li, H., Zhang, J., Kumar, A., Zheng, M., Atherton, S.S., Yu, F.-S.X., Herpes simplex virus 1 infection induces the expression of proinflammatory cytokines, interferons and TLR7 in human corneal epithelial cells (2006) Immunology, 117 (2), pp. 167-176
  • Gamache, D.A., Dan Dimitrijevich, S., Weimer, L.K., Lang, L.S., Spellman, J.M., Graff, G., Yanni, J.M., Secretion of proinflammatory cytokines by human conjunctival epithelial cells (1997) Ocul. Immunol. Inflam., 5 (2), pp. 117-128
  • Biswas, P.S., Banerjee, K., Kim, B., Rouse, B.T., Mice transgenic for IL-1 receptor antagonist protein are resistant to herpetic stromal keratitis: Possible role for IL-1 in herpetic stromal keratitis pathogenesis (2004) J. Immunol., 172 (6), pp. 3736-3744
  • Bouic, P.J.D., Sterols and sterolins: new drugs for the immune system? (2002) Drug. Discov. Today, 7 (14), pp. 775-778
  • Duan, R., Remeijer, L., van Dun, J.M., Osterbaus, A.D.M.E., Verjans, G.M.G.M., Granulocyte macrophage colony-stimulating factor expression in human herpetic stromal keratitis: implications for the role of neutrophils in HSK (2007) Invest. Ophthalmol. Vis. Sci., 48 (1), pp. 277-284
  • Morgensen, T.H., Melchjorsen, J., Malmgaard, L., Casola, A., Paludan, S.R., Suppression of proinflammatory cytokine expression by Herpes Simplex Virus type 1 (2004) J. Virol., 78 (11), pp. 5883-5890
  • Kim, S.H., Kim, J., Sharma, R.P., Inhibition of p38 and ERK MAP kinases blocks endotoxin-induced nitric oxide production and differentially modulates cytokine expression (2004) Pharmacol. Res., 49 (5), pp. 433-439

Citas:

---------- APA ----------
Michelini, F.M., Berra, A. & Alché, L.E. (2008) . The in vitro immunomodulatory activity of a synthetic brassinosteroid analogue would account for the improvement of herpetic stromal keratitis in mice. Journal of Steroid Biochemistry and Molecular Biology, 108(1-2), 164-170.
http://dx.doi.org/10.1016/j.jsbmb.2007.10.002
---------- CHICAGO ----------
Michelini, F.M., Berra, A., Alché, L.E. "The in vitro immunomodulatory activity of a synthetic brassinosteroid analogue would account for the improvement of herpetic stromal keratitis in mice" . Journal of Steroid Biochemistry and Molecular Biology 108, no. 1-2 (2008) : 164-170.
http://dx.doi.org/10.1016/j.jsbmb.2007.10.002
---------- MLA ----------
Michelini, F.M., Berra, A., Alché, L.E. "The in vitro immunomodulatory activity of a synthetic brassinosteroid analogue would account for the improvement of herpetic stromal keratitis in mice" . Journal of Steroid Biochemistry and Molecular Biology, vol. 108, no. 1-2, 2008, pp. 164-170.
http://dx.doi.org/10.1016/j.jsbmb.2007.10.002
---------- VANCOUVER ----------
Michelini, F.M., Berra, A., Alché, L.E. The in vitro immunomodulatory activity of a synthetic brassinosteroid analogue would account for the improvement of herpetic stromal keratitis in mice. J. Steroid Biochem. Mol. Biol. 2008;108(1-2):164-170.
http://dx.doi.org/10.1016/j.jsbmb.2007.10.002