Artículo

Oberkersch, R.; Maccari, F.; Bravo, A.I.; Volpi, N.; Gazzaniga, S.; Calabrese, G.C. "Atheroprotective remodelling of vascular dermatan sulphate proteoglycans in response to hypercholesterolaemia in a rat model" (2014) International Journal of Experimental Pathology. 95(3):181-190
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Abstract:

Proteoglycan accumulation within the arterial intima has been implicated in atherosclerosis progression in humans. Nevertheless, hypercholesterolaemia is unable to induce intimal thickening and atheroma plaque development in rats. The study was performed to analyse proteoglycans modifications in rats fed with a high-cholesterol diet to understand whether vascular wall remodelling protects against lesions. Sections obtained from rat aortas showed normal features, in intimal-to-media ratio and lipid accumulation. However, focal endothelial hyperplasia and neo-intima rearrangement were observed in high-cholesterol animals. Besides, hypercholesterolaemia induced an inflammatory microenviroment. We determined the expression of different proteoglycans from aortic cells by Western blot and observed a diminished production of decorin and biglycan in high-cholesterol animals compared with control (P < 0.01 and P < 0.05, respectively). Versican was increased in high-cholesterol animals (P < 0.05), whereas perlecan production showed no differences. No modification of the total content of glycosaminoglycans (GAGs) was found between the two experimental groups. In contrast, the chondroitin sulphate/dermatan sulphate ratio was increased in the high-cholesterol group as compared to the control (0.56 and 0.34, respectively). Structural alterations in the disaccharide composition of galactosaminoglycans were also detected by HPLC, as the ratio of 6-sulphate to 4-sulphate disaccharides was increased in high-cholesterol animals (P < 0.05). Our results suggest that attenuation of decorin and biglycan expression might be an effective strategy to inhibit the first step in atherogenesis, although specific GAG structural modification associated with the development of vascular disease took place. Results emphasize the potential application of therapies based on vascular matrix remodelling to treat atherosclerosis. © 2014 The Authors. International Journal of Experimental Pathology © 2014 International Journal of Experimental Pathology.

Registro:

Documento: Artículo
Título:Atheroprotective remodelling of vascular dermatan sulphate proteoglycans in response to hypercholesterolaemia in a rat model
Autor:Oberkersch, R.; Maccari, F.; Bravo, A.I.; Volpi, N.; Gazzaniga, S.; Calabrese, G.C.
Filiación:Cátedra de Biología Celular y Molecular, Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín, Ciudad Autónoma de Buenos Aires, Argentina
Department of Life Science, University of Modena and Reggio Emilia, Modena, Italy
Hospital Eva Perón, San Martín, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón, Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Aortic cells; Atherosclerosis; Dermatan sulphate proteoglycans; Extracellular matrix; beta actin; biglycan; chondroitin 6 sulfate; chondroitin sulfate; decorin; dermatan sulfate; galactosaminoglycan; heparan sulfate; high density lipoprotein cholesterol; messenger RNA; perlecan; triacylglycerol; tumor necrosis factor alpha; versican; animal experiment; animal tissue; aorta; article; atherosclerosis; blood vessel wall; cholesterol blood level; cholesterol diet; controlled study; high performance liquid chromatography; hypercholesterolemia; immunochemistry; male; neointima; nonhuman; priority journal; protein expression; rat; reverse transcription polymerase chain reaction; smooth muscle fiber; Western blotting; aortic cells; atherosclerosis; dermatan sulphate proteoglycans; extracellular matrix; Animals; Aorta; Atherosclerosis; Cholesterol; Chondroitin Sulfate Proteoglycans; Dermatan Sulfate; Diet, Atherogenic; Disease Models, Animal; Extracellular Matrix; Gene Expression Regulation; Glycosaminoglycans; Goats; Humans; Hypercholesterolemia; Lipids; Male; Plaque, Atherosclerotic; Rabbits; Rats; Rats, Wistar; Tumor Necrosis Factor-alpha
Año:2014
Volumen:95
Número:3
Página de inicio:181
Página de fin:190
DOI: http://dx.doi.org/10.1111/iep.12072
Título revista:International Journal of Experimental Pathology
Título revista abreviado:Int. J. Exp. Pathol.
ISSN:09599673
CODEN:IJEPE
CAS:biglycan, 123939-84-4; chondroitin 6 sulfate, 25322-46-7; chondroitin sulfate, 9007-28-7, 9082-07-9; dermatan sulfate, 24967-94-0; galactosaminoglycan, 9056-34-2; heparan sulfate, 9050-30-0; perlecan, 143972-95-6; versican, 126968-45-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09599673_v95_n3_p181_Oberkersch

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Citas:

---------- APA ----------
Oberkersch, R., Maccari, F., Bravo, A.I., Volpi, N., Gazzaniga, S. & Calabrese, G.C. (2014) . Atheroprotective remodelling of vascular dermatan sulphate proteoglycans in response to hypercholesterolaemia in a rat model. International Journal of Experimental Pathology, 95(3), 181-190.
http://dx.doi.org/10.1111/iep.12072
---------- CHICAGO ----------
Oberkersch, R., Maccari, F., Bravo, A.I., Volpi, N., Gazzaniga, S., Calabrese, G.C. "Atheroprotective remodelling of vascular dermatan sulphate proteoglycans in response to hypercholesterolaemia in a rat model" . International Journal of Experimental Pathology 95, no. 3 (2014) : 181-190.
http://dx.doi.org/10.1111/iep.12072
---------- MLA ----------
Oberkersch, R., Maccari, F., Bravo, A.I., Volpi, N., Gazzaniga, S., Calabrese, G.C. "Atheroprotective remodelling of vascular dermatan sulphate proteoglycans in response to hypercholesterolaemia in a rat model" . International Journal of Experimental Pathology, vol. 95, no. 3, 2014, pp. 181-190.
http://dx.doi.org/10.1111/iep.12072
---------- VANCOUVER ----------
Oberkersch, R., Maccari, F., Bravo, A.I., Volpi, N., Gazzaniga, S., Calabrese, G.C. Atheroprotective remodelling of vascular dermatan sulphate proteoglycans in response to hypercholesterolaemia in a rat model. Int. J. Exp. Pathol. 2014;95(3):181-190.
http://dx.doi.org/10.1111/iep.12072