Artículo

Perullini, M.; Amoura, M.; Jobbágy, M.; Roux, C.; Livage, J.; Coradin, T.; Bilmes, S.A. "Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach" (2011) Journal of Materials Chemistry. 21(22):8026-8031
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Abstract:

A two-step process relying on cell encapsulation in alginate beads followed by inorganic gelation from colloidal metal oxides was successfully applied to the immobilization of Escherichia coli bacteria in the presence of boehmite and zirconium oxyhydroxide particles. In the case of the Al-based gel, the alginate bead obtained at low biopolymer content provides an efficient barrier against the encapsulation stress. In contrast, an increase in the alginate concentration together with the phosphate-induced mineralization of the biopolymer bead is found necessary to maintain the viability of entrapped bacteria in Zr-based gels. Diffusion studies using model molecular and colloidal species put in evidence that positively charged Zr-oligomers and ZrO 2 nanoparticles may be involved in the cytotoxicity of the precursor solution. This optimization of the encapsulation process allows the first observation of E. coli growth within such metal oxide-alginate hybrid gels. Results presented in this work give a clear evidence that sol-gel based cell encapsulation can now be envisioned within a wide variety of metal oxide hosts through the optimization of the pre-encapsulation environment. © 2011 The Royal Society of Chemistry.

Registro:

Documento: Artículo
Título:Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach
Autor:Perullini, M.; Amoura, M.; Jobbágy, M.; Roux, C.; Livage, J.; Coradin, T.; Bilmes, S.A.
Filiación:INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Pab. II, C1428 EHA Buenos Aires, Argentina
UPMC Univ Paris 06, CNRS, Collège de France, 11 place Marcellin Berthelot, F-75005 Paris, France
Palabras clave:Alginate beads; Cell encapsulations; Colloidal metals; Diffusion studies; E. coli; Escherichia coli bacteria; Hybrid approach; Hybrid gels; Inorganic gelation; Metal oxides; Oxyhydroxides; Positively charged; Precursor solutions; Two-step process; Bacteria; Bacteriology; Biomolecules; Cell immobilization; Coagulation; Escherichia coli; Gelation; Gels; Metallic compounds; Metals; Oligomers; Optimization; Zirconium; Alginate
Año:2011
Volumen:21
Número:22
Página de inicio:8026
Página de fin:8031
DOI: http://dx.doi.org/10.1039/c1jm10684h
Título revista:Journal of Materials Chemistry
Título revista abreviado:J. Mater. Chem.
ISSN:09599428
CODEN:JMACE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09599428_v21_n22_p8026_Perullini

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Citas:

---------- APA ----------
Perullini, M., Amoura, M., Jobbágy, M., Roux, C., Livage, J., Coradin, T. & Bilmes, S.A. (2011) . Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach. Journal of Materials Chemistry, 21(22), 8026-8031.
http://dx.doi.org/10.1039/c1jm10684h
---------- CHICAGO ----------
Perullini, M., Amoura, M., Jobbágy, M., Roux, C., Livage, J., Coradin, T., et al. "Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach" . Journal of Materials Chemistry 21, no. 22 (2011) : 8026-8031.
http://dx.doi.org/10.1039/c1jm10684h
---------- MLA ----------
Perullini, M., Amoura, M., Jobbágy, M., Roux, C., Livage, J., Coradin, T., et al. "Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach" . Journal of Materials Chemistry, vol. 21, no. 22, 2011, pp. 8026-8031.
http://dx.doi.org/10.1039/c1jm10684h
---------- VANCOUVER ----------
Perullini, M., Amoura, M., Jobbágy, M., Roux, C., Livage, J., Coradin, T., et al. Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach. J. Mater. Chem. 2011;21(22):8026-8031.
http://dx.doi.org/10.1039/c1jm10684h