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Perullini, M.; Amoura, M.; Roux, C.; Coradin, T.; Livage, J.; Japas, M.L.; Jobbágy, M.; Bilmes, S.A. "Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors" (2011) Journal of Materials Chemistry. 21(12):4546-4552
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Abstract:

The development of sol-gel based processes for the encapsulation of living cells is a challenging task where the host material must be optimized both in terms of intrinsic properties and impact on cell viability. Here, the mechanical stability of silica hosts obtained via a mixed aqueous route based on sodium silicate-silica nanoparticle mixtures could be improved by increasing the relative content of molecular precursors over colloidal silica. The specific surface area, pore volume and diffusion properties of the host were also modified. Moreover, this resulted in an increase in the ionic strength, inducing high osmolarity that is detrimental to encapsulated Escherichia coli bacteria. Glycine betaine, a well-known E. coli osmoprotector, could be successfully used as an additive to the sol-gel formulation to limit this osmotic stress. Compared to the previously studied glycerol additive, glycine betaine was found more efficient to preserve the bacterial viability. Moreover, this efficiency was obtained with much lower amounts of glycine betaine, whose addition has therefore no detrimental impact on the silica host structure. These data provide new information about the different cellular stresses resulting from the sol-gel encapsulation process and demonstrate the importance of combining chemical and biological approaches to design more robust functional "living" materials. © 2011 The Royal Society of Chemistry.

Registro:

Documento: Artículo
Título:Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors
Autor:Perullini, M.; Amoura, M.; Roux, C.; Coradin, T.; Livage, J.; Japas, M.L.; Jobbágy, M.; Bilmes, S.A.
Filiación:INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pab. II C1428EHA, Buenos Aires, Argentina
Centro Interdisciplinario de Nanociencia y Nanotecnología, Argentina
UPMC Univ Paris 06, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), 11 place Marcelin Berthelot, F-75005 Paris, France
Centro Atómico Constituyentes, CNEA, ECyT-UNSAM, Av. Gral Paz 1499, 1650 Pcia. Buenos Aires, Argentina
Palabras clave:Bacterial viability; Biological approach; Cell viability; Cellular stress; Colloidal silica; Diffusion properties; E. coli; Escherichia coli bacteria; Glycine betaine; Host materials; Host structure; Intrinsic property; Living cell; Molecular precursor; Osmolarity; Osmotic stress; Pore volume; Silica matrix; Silica nanoparticles; Sodium silicate; Sol-gel encapsulation; Amino acids; Bacteriology; Escherichia coli; Gels; Glycerol; Ionic strength; Mechanical stability; Silica; Silicates; Sodium; Sol-gels; Sols; Sol-gel process
Año:2011
Volumen:21
Número:12
Página de inicio:4546
Página de fin:4552
DOI: http://dx.doi.org/10.1039/c0jm03948a
Título revista:Journal of Materials Chemistry
Título revista abreviado:J. Mater. Chem.
ISSN:09599428
CODEN:JMACE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09599428_v21_n12_p4546_Perullini

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Citas:

---------- APA ----------
Perullini, M., Amoura, M., Roux, C., Coradin, T., Livage, J., Japas, M.L., Jobbágy, M.,..., Bilmes, S.A. (2011) . Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors. Journal of Materials Chemistry, 21(12), 4546-4552.
http://dx.doi.org/10.1039/c0jm03948a
---------- CHICAGO ----------
Perullini, M., Amoura, M., Roux, C., Coradin, T., Livage, J., Japas, M.L., et al. "Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors" . Journal of Materials Chemistry 21, no. 12 (2011) : 4546-4552.
http://dx.doi.org/10.1039/c0jm03948a
---------- MLA ----------
Perullini, M., Amoura, M., Roux, C., Coradin, T., Livage, J., Japas, M.L., et al. "Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors" . Journal of Materials Chemistry, vol. 21, no. 12, 2011, pp. 4546-4552.
http://dx.doi.org/10.1039/c0jm03948a
---------- VANCOUVER ----------
Perullini, M., Amoura, M., Roux, C., Coradin, T., Livage, J., Japas, M.L., et al. Improving silica matrices for encapsulation of Escherichiacoli using osmoprotectors. J. Mater. Chem. 2011;21(12):4546-4552.
http://dx.doi.org/10.1039/c0jm03948a