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Abstract:

A comprehensive analysis of the carbohydrate-containing macromolecules from the coencocytic green seaweed Codium fragile and their arrangement in the cell wall was carried out. Cell walls in this seaweed are highly complex structures composed of 31% (w/w) of linear (1→4)-β-D-mannans, 9% (w/w) of pyruvylated arabinogalactan sulfates (pAGS), and low amounts of hydroxyproline rich-glycoprotein epitopes (HRGP). In situ chemical imaging by synchrotron radiation Fourier transform infrared (SR-FTIR) microspectroscopy and by immunolabeling using antibodies against specific cell wall carbohydrate epitopes revealed that β-D-mannans and pAGS are placed in the middle part of the cell wall, whereas HRGP epitopes (arabinogalactan proteins (AGPs) and extensins) are located on the wall boundaries, especially in the utricle apical zone. pAGS are sulfated at C-2 and/or C-4 of the 3-linked β-L-arabinopyranose units and at C-4 and/or C-6 of the 3-linked β-D-galactopyranose residues. In addition, high levels of ketals of pyruvic acid were found mainly at 3,4- of some terminal β-D-Gal p units forming a five-membered ring. Ramification was found at some C-6 of the 3-linked β-D-Gal p units. In agreement with the immunolabeled AGP epitopes, a nonsulfated branched furanosidic arabinan with 5-linked α-L-Ara f, 3,5-linked α-L-Ara f, and terminal α-L-Ara f units and a nonsulfated galactan structure composed of 3-(3,6)-linked β-D-Gal p residues, both typical of type-II AG glycans were found, suggesting that AGP structures are present at low levels in the cell walls of this seaweed. Based on this study, it is starting to emerge that Codium has developed unique cell wall architecture, when compared, not only with that of vascular plants, but also with other related green seaweeds and algae. © The Author 2008. Published by Oxford University Press. All rights reserved.

Registro:

Documento: Artículo
Título:Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile
Autor:Estevez, J.M.; Fernández, P.V.; Kasulin, L.; Dupree, P.; Estevez, J.M.
Filiación:Carnegie Institution of Washington, Plant Biology, Stanford University, Stanford, CA 94305, United States
Cátedra de Química de Biomoléculas, Departamento de Biología Aplicada y Alimentos (CIHIDECAR-CONICET), Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE Buenos Aires, Argentina
Department of Biochemistry, University of Cambridge, Building O, Downing site, Cambridge CB2 1QW, United Kingdom
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina
National Research Council of Argentina (CONICET), Argentina
Palabras clave:β-mannan; Codium cell wall; Green seaweed; HRGP epitope; Pyruvylated arabinogalactan sulfate; 3 linked beta levo arabinopyranose; 3,5 linked alpha levo Araf; 5 linked alpha levo Araf; arabinogalactan sulfate; epitope; extensin; hydroxyproline rich glycoprotein; mannan; oligosaccharide; pyruvic acid; terminal alpha levo Araf; unclassified drug; alga; antibody labeling; article; carbohydrate analysis; cell structure; cell wall; Codium fragile; controlled study; cytoarchitecture; epitope mapping; infrared spectroscopy; macromolecule; nonhuman; priority journal; protein localization; residue analysis; seaweed; structure analysis; synchrotron; Cell Wall; Epitopes; Galactans; Hydroxyproline; Mannans; Nuclear Magnetic Resonance, Biomolecular; Seaweed; Spectroscopy, Fourier Transform Infrared; Sulfates; Synchrotrons; algae; Ara; Codium; Codium fragile; Tracheophyta
Año:2009
Volumen:19
Número:3
Página de inicio:212
Página de fin:228
DOI: http://dx.doi.org/10.1093/glycob/cwn101
Título revista:Glycobiology
Título revista abreviado:Glycobiology
ISSN:09596658
CODEN:GLYCE
CAS:mannan, 51395-96-1, 9036-88-8; pyruvic acid, 127-17-3, 19071-34-2, 57-60-3; Epitopes; Galactans; Hydroxyproline, 51-35-4; Mannans; Sulfates; arabinogalactan, 9036-66-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09596658_v19_n3_p212_Estevez

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Citas:

---------- APA ----------
Estevez, J.M., Fernández, P.V., Kasulin, L., Dupree, P. & Estevez, J.M. (2009) . Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile. Glycobiology, 19(3), 212-228.
http://dx.doi.org/10.1093/glycob/cwn101
---------- CHICAGO ----------
Estevez, J.M., Fernández, P.V., Kasulin, L., Dupree, P., Estevez, J.M. "Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile" . Glycobiology 19, no. 3 (2009) : 212-228.
http://dx.doi.org/10.1093/glycob/cwn101
---------- MLA ----------
Estevez, J.M., Fernández, P.V., Kasulin, L., Dupree, P., Estevez, J.M. "Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile" . Glycobiology, vol. 19, no. 3, 2009, pp. 212-228.
http://dx.doi.org/10.1093/glycob/cwn101
---------- VANCOUVER ----------
Estevez, J.M., Fernández, P.V., Kasulin, L., Dupree, P., Estevez, J.M. Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile. Glycobiology. 2009;19(3):212-228.
http://dx.doi.org/10.1093/glycob/cwn101