Abstract:
Galectin-1 (Gal-1) is a widely expressed β-galactoside-binding protein that exerts pleiotropic biological functions. To gain insight into the potential role of Gal-1 as a novel modulator of Leydig cells, we investigated its effect on the growth and death of MA-10 tumor Leydig cells. In this study, we identified cytoplasmic Gal-1 expression in these tumor cells by cytofluorometry. DNA fragmentation, caspase-3, -8, and -9 activation, loss of mitochondrial membrane potential (ΔΨ m), cytochrome c (Cyt c) release, and FasL expression suggested that relatively high concentrations of exogenously added recombinant Gal-1 (rGal-1) induced apoptosis by the mitochondrial and death receptor pathways. These pathways were independently activated, as the presence of the inhibitor of caspase-8 or -9 only partially prevented Gal-1-effect. On the contrary, low concentrations of Gal-1 significantly promoted cell proliferation, without inducing cell death. Importantly, the presence of the disaccharide lactose prevented Gal-1 effects, suggesting the involvement of the carbohydrate recognition domain (CRD). This study provides strong evidence that Gal-1 is a novel biphasic regulator of Leydig tumor cell number, suggesting a novel role for Gal-1 in the reproductive physiopathology. © Copyright 2006 Oxford University Press.
Registro:
Documento: |
Artículo
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Título: | Galectin-1: Biphasic growth regulation of Leydig tumor cells |
Autor: | Biron, V.A.; Iglesias, M.; Troncoso, M.F.; Besio-Moreno, M.; Patrignani, Z.J.; Pignataro, O.P.; Wolfenstein-Todel, C. |
Filiación: | Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina Laboratorio de Endocrinología Molecular y Transducción de Señales, Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, 1428 Buenos Aires, Argentina
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Palabras clave: | Apoptosis; Galectin-1; Leydig cells; Proliferation; caspase 3; caspase 8; caspase 8 inhibitor; caspase 9; caspase 9 inhibitor; cytochrome c; death receptor; disaccharide; DNA fragment; FAS ligand; galectin 1; lactose; animal cell; apoptosis; article; carbohydrate analysis; cell growth; cell membrane potential; cell proliferation; controlled study; cytofluorometry; enzyme activation; growth regulation; Leydig cell; Leydig cell tumor; male; mitochondrial membrane; mouse; nonhuman; pathophysiology; priority journal; protein determination; protein expression; protein function; protein secretion; Cell Line, Tumor; Cell Proliferation; Cytoplasm; DNA Fragmentation; Dose-Response Relationship, Drug; Galectin 1; Gene Expression Regulation, Neoplastic; Humans; Lactose; Leydig Cells; Male; Membrane Potentials; Mitochondria; Neoplasm Proteins; Sertoli-Leydig Cell Tumor; Testicular Neoplasms |
Año: | 2006
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Volumen: | 16
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Número: | 9
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Página de inicio: | 810
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Página de fin: | 821
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DOI: |
http://dx.doi.org/10.1093/glycob/cwl013 |
Título revista: | Glycobiology
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Título revista abreviado: | Glycobiology
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ISSN: | 09596658
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CODEN: | GLYCE
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CAS: | caspase 3, 169592-56-7; caspase 9, 180189-96-2; cytochrome c, 9007-43-6, 9064-84-0; galectin 1, 258495-34-0; lactose, 10039-26-6, 16984-38-6, 63-42-3, 64044-51-5; Galectin 1; Lactose, 63-42-3; Neoplasm Proteins
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PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_09596658_v16_n9_p810_Biron.pdf |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09596658_v16_n9_p810_Biron |
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Citas:
---------- APA ----------
Biron, V.A., Iglesias, M., Troncoso, M.F., Besio-Moreno, M., Patrignani, Z.J., Pignataro, O.P. & Wolfenstein-Todel, C.
(2006)
. Galectin-1: Biphasic growth regulation of Leydig tumor cells. Glycobiology, 16(9), 810-821.
http://dx.doi.org/10.1093/glycob/cwl013---------- CHICAGO ----------
Biron, V.A., Iglesias, M., Troncoso, M.F., Besio-Moreno, M., Patrignani, Z.J., Pignataro, O.P., et al.
"Galectin-1: Biphasic growth regulation of Leydig tumor cells"
. Glycobiology 16, no. 9
(2006) : 810-821.
http://dx.doi.org/10.1093/glycob/cwl013---------- MLA ----------
Biron, V.A., Iglesias, M., Troncoso, M.F., Besio-Moreno, M., Patrignani, Z.J., Pignataro, O.P., et al.
"Galectin-1: Biphasic growth regulation of Leydig tumor cells"
. Glycobiology, vol. 16, no. 9, 2006, pp. 810-821.
http://dx.doi.org/10.1093/glycob/cwl013---------- VANCOUVER ----------
Biron, V.A., Iglesias, M., Troncoso, M.F., Besio-Moreno, M., Patrignani, Z.J., Pignataro, O.P., et al. Galectin-1: Biphasic growth regulation of Leydig tumor cells. Glycobiology. 2006;16(9):810-821.
http://dx.doi.org/10.1093/glycob/cwl013