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Abstract:

The effects of the membrane permeant Ca2+ chelator BAPTA-AM on voltage-gated Na+, Ca2+, K+ (I(Na), I(Ca), I(K), respectively) and Ca2+-activated K+ (I(KCa)) currents in cultured bovine chromaffin cells were investigated using the whole-cell patch-clamp technique. Superfusion with BAPTA-AM (50 μM) induced a rapid (< 60 s) and reversible block of both I(KCa) and I(K) (~50%), without affecting either I(Ca) or I(Na). Preincubation with BAPTA-AM (50 μM, 30 min) or cell loading with the non- permeable active form of BAPTA (10 mM in the pipette solution) permanently blocked I(KCa). BAPTA-AM superfusion (50 μM) also blocked I(K) (~53%) after BAPTA-loading or BAPTA-AM preincubation. In conclusion, we show a fast and reversible block of I(KCa) and I(K) by BAPTA-AM, acting directly on K+ channels before it operates as a Ca2+ chelator, in cultured bovine chromaffin cells.

Registro:

Documento: Artículo
Título:BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells
Autor:Urbano, F.J.; Buño, W.
Filiación:Inst. Santiago Ramón y Cajal, CSIC, Avenida Doctor Arce 37, 28002-Madrid, Spain
Lab. de Fisiol. y Biol. Molecular, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428-Buenos Aires, Argentina
Palabras clave:BAPTA; BAPTA-AM; Extracellular block; Open channel block; Potassium current block; animal cell; article; calcium transport; cattle; channel gating; chromaffin cell; nonhuman; patch clamp; potassium current; priority journal
Año:1998
Volumen:9
Número:15
Página de inicio:3403
Página de fin:3407
DOI: http://dx.doi.org/10.1097/00001756-199810260-00013
Título revista:NeuroReport
Título revista abreviado:NeuroReport
ISSN:09594965
CODEN:NERPE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v9_n15_p3403_Urbano

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Citas:

---------- APA ----------
Urbano, F.J. & Buño, W. (1998) . BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells. NeuroReport, 9(15), 3403-3407.
http://dx.doi.org/10.1097/00001756-199810260-00013
---------- CHICAGO ----------
Urbano, F.J., Buño, W. "BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells" . NeuroReport 9, no. 15 (1998) : 3403-3407.
http://dx.doi.org/10.1097/00001756-199810260-00013
---------- MLA ----------
Urbano, F.J., Buño, W. "BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells" . NeuroReport, vol. 9, no. 15, 1998, pp. 3403-3407.
http://dx.doi.org/10.1097/00001756-199810260-00013
---------- VANCOUVER ----------
Urbano, F.J., Buño, W. BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells. NeuroReport. 1998;9(15):3403-3407.
http://dx.doi.org/10.1097/00001756-199810260-00013