Navegar

Colección

Datos Estadísticas

Artículo

Yakisich, J.S.; Boethius, J.; Lindblom, I.O.; Wallstedt, L.; Vargas, V.I.; Sidén, A.; Cruz, M.H. "Inhibition of DNA synthesis in human gliomas by roscovitine" (1999) NeuroReport. 10(12):2563-2567
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The early effect of 1-100 μM roscovitine, a purine analogue and cyclin- dependent kinase inhibitor, was studied on tissue specimens from eight human malignant gliomas. The tissue was incubated immediately after resection with DMEM containing [3H]methyl-thymidine plus vehicle alone or the proper concentration of roscovitine for 33-90 min. The DNA synthesis rate was assessed by measurement of [3H]methyl-thymidine incorporation into trichloroacetic acid insoluble material/mg protein/min. In all gliomas, 100 μM roscovitine inhibited DNA synthesis by 71-97% (average 89 ± 8%, p < 0.0001). This inhibitory effect of roscovitine appeared within 30 min of incubation and was concentration dependent.

Registro:

Documento: Artículo
Título:Inhibition of DNA synthesis in human gliomas by roscovitine
Autor:Yakisich, J.S.; Boethius, J.; Lindblom, I.O.; Wallstedt, L.; Vargas, V.I.; Sidén, A.; Cruz, M.H.
Filiación:Applied Biochemistry, Clinical Research Center, Huddinge University Hospital, S-141 86 Huddinge, Sweden
Inst. de Invest. Bioquimicas F., FCEyN, UBA, Buenos Aires, Argentina
Department of Neurology, Karolinska Institute, Novum, Huddinge University Hospital, S-141 86 Huddinge, Sweden
Palabras clave:Brain tumors; Cyclin-dependent kinases; DNA synthesis; Gliomas; Proliferation rate; Roscovitine; lomustine; roscovitine; adult; article; concentration response; DNA synthesis inhibition; drug effect; female; glioblastoma; glioma; human; human tissue; male; priority journal
Año:1999
Volumen:10
Número:12
Página de inicio:2563
Página de fin:2567
DOI: http://dx.doi.org/10.1097/00001756-199908200-00023
Título revista:NeuroReport
Título revista abreviado:NeuroReport
ISSN:09594965
CODEN:NERPE
CAS:lomustine, 13010-47-4; roscovitine, 186692-46-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v10_n12_p2563_Yakisich

Referencias:

  • Kleihues, P., Burger, P.C., Scheithauer, B.W., (1993) Histological Typing of Tumors of the Central Nervous System, , Berlin: Springer-Verlag
  • Burger, P.C., Green, S.B., (1987) Cancer, 59, pp. 1617-1625
  • Davis, F.G., Freels, S., Grutsch, J., (1998) J Neurosurg, 88, pp. 1-10
  • Lunsford, L.D., Somaza, S., Kondziotka, D., (1995) Clin Neurosurg, 42, pp. 464-479
  • Barker, F.G., Chang, S.M., Gutin, P.H., (1998) Neurosurgery, 42, pp. 709-723
  • Jung, S., Rutka, J.T., Hinek, A., (1998) J Neuropathol Exp Neurol, 57, pp. 439-448
  • Deen, F.D., Chiarodo, A., Grimm, E.A., (1993) J Neurooncol, pp. 243-272
  • Costello, J.F., Plass, C., Arap, W., (1997) Cancer Res, 57, pp. 1250-1254
  • Dirks, P.B., Hubbard, S.L., Murakami, M., (1997) J Neuropathol Exp Neurol, 56, pp. 291-300
  • Olson, J.J., Barnett, D., Yang, J., (1998) Clin Cancer Res, 4, pp. 215-222
  • Ono, Y., Tamiya, T., Ichikawa, T., (1996) J Neuropathol Exp Neurol, 55, pp. 1026-1031
  • Rollbrocker, B., Waha, A., Louis, D.N., (1996) Acta Neuropathol (Berl), 92, pp. 70-74
  • Srivenugopal, K.S., Ali-Osman, F., (1996) Oncogene, 12, pp. 2029-2034
  • Meijer, L., Borgne, A., Mulner, O., (1997) Eur J Biochem, 243, pp. 527-536
  • Schutte, B., Nieland, L., Van Engeland, M., (1997) Exp Cell Res, 236, pp. 4-15
  • Pippin, J.W., Qu, Q., Meijer, L., (1997) J Clin Lnvest, 100, pp. 2512-2520
  • Yakisich, J.S., Sidén, A., Idoyaga Vargas, V., (1998) Biochem Biophys Res Commun, 243, pp. 674-677
  • Altman, J., Das, G.D., (1996) J Comp Neurol, 126, pp. 337-390
  • Yakisich, J.S., Sidén, A., Idoyaga Vargas, V., (1996) Exp Neurol, 151, pp. 194-202
  • Vilenchik, M.M., Tretjak, T.M., (1977) J Neurochem, 29, pp. 1159-1161
  • Park, D.S., Farinelli, S.E., Greene, L.A., (1996) J Biol Chem, 271, pp. 6161-8169
  • Meijer, L., (1996) Trends Cell Biol, 6, pp. 393-397
  • Abraham, R.T., Acquarone, M., Andersen, A., (1995) Biol Cell, 83, pp. 105-120
  • Dirks, P.B., Rutka, J.T., (1998) Neurosurgery, 40, pp. 1000-1015
  • Maas J.W., Jr., Horstmann, S., Borasio, G.D., (1998) J Neurochem, 70, pp. 1401-1410

Citas:

---------- APA ----------
Yakisich, J.S., Boethius, J., Lindblom, I.O., Wallstedt, L., Vargas, V.I., Sidén, A. & Cruz, M.H. (1999) . Inhibition of DNA synthesis in human gliomas by roscovitine. NeuroReport, 10(12), 2563-2567.
http://dx.doi.org/10.1097/00001756-199908200-00023
---------- CHICAGO ----------
Yakisich, J.S., Boethius, J., Lindblom, I.O., Wallstedt, L., Vargas, V.I., Sidén, A., et al. "Inhibition of DNA synthesis in human gliomas by roscovitine" . NeuroReport 10, no. 12 (1999) : 2563-2567.
http://dx.doi.org/10.1097/00001756-199908200-00023
---------- MLA ----------
Yakisich, J.S., Boethius, J., Lindblom, I.O., Wallstedt, L., Vargas, V.I., Sidén, A., et al. "Inhibition of DNA synthesis in human gliomas by roscovitine" . NeuroReport, vol. 10, no. 12, 1999, pp. 2563-2567.
http://dx.doi.org/10.1097/00001756-199908200-00023
---------- VANCOUVER ----------
Yakisich, J.S., Boethius, J., Lindblom, I.O., Wallstedt, L., Vargas, V.I., Sidén, A., et al. Inhibition of DNA synthesis in human gliomas by roscovitine. NeuroReport. 1999;10(12):2563-2567.
http://dx.doi.org/10.1097/00001756-199908200-00023