Artículo

Bianchini, G.M.; Carricarte, V.C.; Flawia, M.M.; Sanchez-Rivas, C. "Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation" (1993) World Journal of Microbiology and Biotechnology. 9(2):168-173
La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Six Rhizobium meliloti mutants were isolated after Tn5-mediated mutagenesis as resistant to inhibition by a mixture of amino acids (serine, methionine, glycine and leucine). All were defective in adenylate cyclase activity and failed to form nodules in infected roots of Medicago sativa. Furthermore, like other nodulation mutants, they showed altered motility and increased secretion of exopolysaccharides; addition of cAMP to the growth medium abolished some of these phenotypic defects. The possibility that adenylate cyclase participates in the transduction of signals inducing nodulation is discussed. © 1993 Rapid Communications of Oxford Ltd.

Registro:

Documento: Artículo
Título:Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation
Autor:Bianchini, G.M.; Carricarte, V.C.; Flawia, M.M.; Sanchez-Rivas, C.
Filiación:Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI-CONICET), Obligado 2490, Buenos Aires, 1428, Argentina
Facultad de Ciencias Universitaria Naturales, Departamento de Quimica Biologica, Ciudad Universitaria Pabellon II, 4Piso, Buenos Aires, 1428, Argentina
Palabras clave:Adenylate cyclase; amino acid sensitivity; exopolysaccharide; nodulation; Rhizobium meliloti; Tn5 mutagenesis
Año:1993
Volumen:9
Número:2
Página de inicio:168
Página de fin:173
DOI: http://dx.doi.org/10.1007/BF00327829
Título revista:World Journal of Microbiology and Biotechnology
Título revista abreviado:World J Microbiol Biotechnol
ISSN:09593993
CODEN:WJMBE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v9_n2_p168_Bianchini

Referencias:

  • Alper, M.D., Ames, B.N., Transport of antibiotics and metabolite analogs by systems under cyclic AMP control: positive selection of Salmonella typhimurium cya and crp mutants (1978) Journal of Bacteriology, 133, pp. 149-157
  • Beringer, J.E., Beynon, J.L., Buchanan-Wollaston, A.V., Johnston, A.W.B., Transfer of the drug-resistance transposon Tn5 to Rhizobium (1978) Nature, 276, pp. 633-634
  • Beuve, A., Boesten, B., Crasnier, M., Danchin, A., O'Gara, F., Rhizobium meliloti adenylate cyclase is related to eukaryotic adenylate and guanylate cyclases (1990) Journal of Bacteriology, 172, pp. 2614-2621
  • Borthakur, D., Barber, C.E., Lamb, J.W., Daniels, M.J., Downie, J.A., Johnston, A.W.B., A mutation that blocks exopolysaccharide synthesis prevents nodulation of peas by Rhizobium leguminosarum but not of beans by R. phaseoli and is corrected by cloned DNA from Rhizobium or the phytopathogen Xanthomonas (1986) Molecular and General Genetics, 203, pp. 320-323
  • Cangelosi, G.A., Lynn Hung, V., Puvanesarajah, G., Stacey, D.A., Ozga, D.A., Leigh, J.A., Nester, E.W., Common loci for Agrobacterium tumefaciens and Rhizobium meliloti exopolysaccharide synthesis and their roles in plant interactions (1987) Journal of Bacteriology, 169, pp. 2086-2091
  • Carricarte, V.C. 1988. Adenilato ciclasa en plantas superiores: caracterización y purificación de esta enzima en Rhizobium meliloti, nodulos radicalares y Medicago sativa. Thesis, Universidad de Buenos Aires; Catanase, A.C., Emerich, D.W., Zahler, W.L., Adenylate cyclase and cyclic AMP phosphodiesterase in Bradyrhizobium japonicum bacteroids (1989) Journal of Bacteriology, 171, pp. 4531-4536
  • Daniel, J., Danchin, A., Involvement of cyclic AMP and its receptor protein in the sensitivity of Escherichia coli K12 towards serine (1979) Molecular and General Genetics, 176, pp. 343-350
  • Doherty, D., Leigh, J.A., Glazerbrook, J., Walker, G.C., Rhizobium meliloti mutants that overproduce the R. meliloti acidic Calcofluor-binding exopolysaccharide (1988) Journal of Bacteriology, 170, pp. 4249-4256
  • Downie, J.A., Johnston, A.W.B., Nodulation of legumes by Rhizobium: the recognized root? (1986) Cell, 47, pp. 153-154
  • Dylon, T., Ielpi, L., Standfield, S., Koshyap, L., Douglas, C., Yanofsky, M., Nester, E., Ditta, G., Rhizobium meliloti genes required for nodule development are related to chromosomal virulence genes in Agrobacterium tumefaciens (1986) Proceedings of the National Academy of Sciences of the United States of America, 83, pp. 4403-4407
  • Firmin, J.L., Wilson, K.E., Rossen, L., Johnston, A.W.B., Flavonoid activation of nodulation genes in Rhizobium reversed by other compounds present in plants (1986) Nature, 324, pp. 90-92
  • Flawia, M.M., Torres, H.N., Adenylate cyclase activity in Neurospora crassa (1972) Journal of Biological Chemistry, 247, pp. 6873-6879
  • Geremia, R.A., Cavaignac, S., Zorreguieta, A., Toro, N., Olivares, J., Ugalde, R.A., A Rhizobium meliloti mutant that forms ineffective pseudonodules in alfalfa produces exopolysaccharide but fails to form β-(1–2)glucan (1987) Journal of Bacteriology, 169, pp. 880-884
  • Gottfert, M., Horvath, B., Kondorosi, E., Putnoky, P., Rodriguez-Quinones, Kondorosi, A., At least two nodD genes are necessary for efficient nodulation on alfalfa by Rhizobium meliloti (1986) Journal of Molecular Microbiology, 128, pp. 789-798
  • Gyorgypal, A., Iyer, N., Kondorosi, A., Three regulatory nodD alleles of diverged flavonoid specificity are involved in host-dependent nodulation by Rhizobium meliloti (1988) MGG Molecular & General Genetics, 212, pp. 85-92
  • Keller, M., Muller, P., Simon, R., Puhler, A., Rhizobium meliloti genes for exopolysaccharide synthesis and nodule infection located on megaplasmid are actively transcribed during symbiosis (1988) Molecular Plant-Microbe Interactions, 1, pp. 267-274
  • Kiely, B., O'Gara, F., Cyclic 3,5,adenosine monophosphate synthesis in Rhizobium: identification of a cloned sequence from Rhizobium meliloti coding for adenyl cyclase (1983) Molecular and General Genetics, 192, pp. 230-239
  • Leigh, J.A., Reed, J.W., Hanks, J.F., Hirschand, A.M., Walker, G.C., Rhizobium meliloti mutants that fail to succinylate their Calcofluor-binding exopolysaccharide are defective in nodule invasion (1987) Cell, 51, pp. 579-587
  • Lim, S.T., Shanmugan, K.T., Regulation of hydrogen utilization in Rhizobium japonicum by cyclic AMP (1979) Biochimica et Biophysica Acta, 584, pp. 479-492
  • McGetrick, A.M., Goulding, C.F., Sundaram, S.M., O'Gara, F., Catabolite repression and role of cyclic AMP in CO<inf>2</inf> fixation and H<inf>2</inf> metabolism in Rhizobium spp. (1985) Journal of Bacteriology, 163, pp. 1282-1284
  • Miller, J.H., (1972) Experiments in Molecular Genetics, pp. 431-432. , Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
  • Muller, P., Hynes, M., Kapp, D., Niehaus, K., Puhler, A., The two classes of Rhizobium meliloti infection mutants differ in exopolysaccharide production and in co-inoculation properties with nodulation mutants (1988) Molecular and General Genetics, 211, pp. 17-26
  • O'Regan, M., Kiely, B., O'Gara, F., Expression of the adenyl cyclase-encoding gene (cya) of Rhizobium meliloti F43: existence of two cya genes? (1989) Gene, 83, pp. 243-249
  • Paau, A.S., Leps, W.T., Brill, W.J., Agglutinin from alfalfa necessary for binding and nodulation by Rhizobium meliloti (1981) Science, 213, pp. 1513-1524
  • Peters, N.K., Frost, J.W., Long, S.R., A plant flavonone, luteolin, induces expression of Rhizobium meliloti nodulation genes (1986) Science, 233, pp. 977-980
  • Saier, M.H., Jr., Schmidt, M.R., Leibowitz, M., Cyclic AMP-dependent synthesis of fimbriae in Salmonella typhimurum: effects of cya and pts mutations (1978) Journal of Bacteriology, 145, pp. 489-493
  • Salomon, Y., Londos, C., Rodbell, M., A highly sensitive adenylate cyclase assay (1974) Annals of Biochemistry, 58, pp. 541-548
  • Selvaraj, G., Iyer, V.N., Transposon Tn5 specifies streptomycin resistance in Rhizobium spp (1984) Journal of Bacteriology, 158, pp. 580-589
  • Upchurch, R.G., Elkan, G.H., The role of ammonia, L-glutamate, and cyclic adenosine 3′,5′-monophosphate in the regulation of ammonia assimilation in Rhizobium japonicum (1978) Biochimica et Biophysica Acta, 538, pp. 244-248
  • Vincent, J.M., (1975) Manual for the Practical Study of the Root Nodule Bacteria, pp. 75-76. , IBP Handbook, 15, Blackwell Scientific, Oxford

Citas:

---------- APA ----------
Bianchini, G.M., Carricarte, V.C., Flawia, M.M. & Sanchez-Rivas, C. (1993) . Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation. World Journal of Microbiology and Biotechnology, 9(2), 168-173.
http://dx.doi.org/10.1007/BF00327829
---------- CHICAGO ----------
Bianchini, G.M., Carricarte, V.C., Flawia, M.M., Sanchez-Rivas, C. "Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation" . World Journal of Microbiology and Biotechnology 9, no. 2 (1993) : 168-173.
http://dx.doi.org/10.1007/BF00327829
---------- MLA ----------
Bianchini, G.M., Carricarte, V.C., Flawia, M.M., Sanchez-Rivas, C. "Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation" . World Journal of Microbiology and Biotechnology, vol. 9, no. 2, 1993, pp. 168-173.
http://dx.doi.org/10.1007/BF00327829
---------- VANCOUVER ----------
Bianchini, G.M., Carricarte, V.C., Flawia, M.M., Sanchez-Rivas, C. Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation. World J Microbiol Biotechnol. 1993;9(2):168-173.
http://dx.doi.org/10.1007/BF00327829