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Abstract:

In this work a new Bacillus sp. strain, isolated from honey, was characterized phylogenetically. Its antibacterial activity against three relevant foodborne pathogenic bacteria was studied; the main bioactive metabolites were analyzed using ultraviolet matrix assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Bacillus CBMDC3f was phylogenetically characterized as Bacillus subtilis subsp. subtilis after rRNA analysis of the 16S subunit and the gyrA gene (access codes Genbank JX120508 and JX120516, respectively). Its antibacterial potential was evaluated against Listeria monocytogenes (9 strains), B. cereus (3 strains) and Staphylococcus aureus ATCC29213. Its cell suspension and cell-free supernatant (CFS) exerted significant anti-Listeria and anti-S. aureus activities, while the lipopeptides fraction (LF) also showed anti-B. cereus effect. The UV-MALDI-MS analysis revealed surfactin, iturin and fengycin in the CFS, whereas surfactin predominated in the LF. The CFS from CBMDC3f contained surfactin, iturin and fengycin with four, two and four homologues per family, respectively, whereas four surfactin, one iturin and one fengycin homologues were identified in the LF. For some surfactin homologues, their UV-MALDI-TOF/TOF (MS/MS; Laser Induced Decomposition method, LID) spectra were also obtained. Mass spectrometry analysis contributed with relevant information about the type of lipopeptides that Bacillus strains can synthesize. From our results, surfactin would be the main metabolite responsible for the antibacterial effect. © 2015, Springer Science+Business Media Dordrecht.

Registro:

Documento: Artículo
Título:Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds
Autor:Torres, M.J.; Petroselli, G.; Daz, M.; Erra-Balsells, R.; Audisio, M.C.
Filiación:Instituto de Investigaciones para la Industria Química (INIQUI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Salta, Av.Bolivia 5150, Salta, 4400, Argentina
CIHIDECAR-CONICET, Departamento de Química Orgánica, Universidad de Buenos Aires, Pabellón II, 3 Ciudad Universitaria, Buenos Aires, 1428, Argentina
Palabras clave:Bacillus subtilis subsp. subtilis; Foodborne pathogens; Lipopeptide homologues; Surfactin; UV-MALDI-TOF MS; Bacteria; Bacteriology; Cell culture; Diseases; Inductively coupled plasma; Listeria; Mass spectrometry; Metabolites; Pathogens; Polypeptides; RNA; Spectrometry; Strain; Food-borne pathogens; Lipopeptides; MALDI TOF MS; Subtilis; Surfactin; Food microbiology; antiinfective agent; bacterial DNA; biological product; DNA topoisomerase (ATP hydrolysing); ribosome DNA; RNA 16S; antibiosis; Bacillus subtilis; chemistry; classification; cluster analysis; DNA sequence; food control; genetics; Gram positive bacterium; growth, development and aging; isolation and purification; mass spectrometry; metabolism; molecular genetics; phylogeny; physiology; ultraviolet spectrophotometry; Anti-Bacterial Agents; Antibiosis; Bacillus subtilis; Biological Products; Cluster Analysis; DNA Gyrase; DNA, Bacterial; DNA, Ribosomal; Food Microbiology; Gram-Positive Bacteria; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Ultraviolet
Año:2015
Volumen:31
Número:6
Página de inicio:929
Página de fin:940
DOI: http://dx.doi.org/10.1007/s11274-015-1847-9
Título revista:World Journal of Microbiology and Biotechnology
Título revista abreviado:World J. Microbiol. Biotechnol.
ISSN:09593993
CODEN:WJMBE
CAS:DNA topoisomerase (ATP hydrolysing); Anti-Bacterial Agents; Biological Products; DNA Gyrase; DNA, Bacterial; DNA, Ribosomal; RNA, Ribosomal, 16S
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v31_n6_p929_Torres

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Citas:

---------- APA ----------
Torres, M.J., Petroselli, G., Daz, M., Erra-Balsells, R. & Audisio, M.C. (2015) . Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds. World Journal of Microbiology and Biotechnology, 31(6), 929-940.
http://dx.doi.org/10.1007/s11274-015-1847-9
---------- CHICAGO ----------
Torres, M.J., Petroselli, G., Daz, M., Erra-Balsells, R., Audisio, M.C. "Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds" . World Journal of Microbiology and Biotechnology 31, no. 6 (2015) : 929-940.
http://dx.doi.org/10.1007/s11274-015-1847-9
---------- MLA ----------
Torres, M.J., Petroselli, G., Daz, M., Erra-Balsells, R., Audisio, M.C. "Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds" . World Journal of Microbiology and Biotechnology, vol. 31, no. 6, 2015, pp. 929-940.
http://dx.doi.org/10.1007/s11274-015-1847-9
---------- VANCOUVER ----------
Torres, M.J., Petroselli, G., Daz, M., Erra-Balsells, R., Audisio, M.C. Bacillus subtilis subsp. subtilis CBMDC3f with antimicrobial activity against Gram-positive foodborne pathogenic bacteria: UV-MALDI-TOF MS analysis of its bioactive compounds. World J. Microbiol. Biotechnol. 2015;31(6):929-940.
http://dx.doi.org/10.1007/s11274-015-1847-9