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Abstract:

Cultivation of specialty mushrooms on lignocellulosic wastes represents one of the most economical organic recycling processes. Compared with other cultivated mushrooms, very little is known about the nature of the lignocellulolytic enzymes produced by the edible and medicinal fungus Grifola frondosa, the parameters affecting their production, and enzyme activity profiles during different stages of the developmental cycle. In this work we investigated the enzymes that enable G. frondosa, to colonize and deconstruct two formulations based on industrial lignocellulosic by-products. G. frondosa degraded both substrates (oak-sawdust plus corn bran, and oak/corn bran supplemented with coffee spent-ground) decreasing 67 and 50% of their lignin content, along with 44 and 37% of the polysaccharides (hemicellulose and cellulose) respectively. 35.3% biological efficiency was obtained when using oak sawdust plus corn bran as substrate. Coffee spent-ground addition inhibited mushroom production, decreased growth, xylanase and cellulase activities. However, taking into account that G. frondosa successfully colonized this residue; this substrate formula might be considered for its growth and medicinal polysaccharide production. Although G. frondosa tested positive for Azure B plate degradation, a qualitative assay for lignin-peroxidase, attempts to detect this activity during solid state fermentation were unsuccessful. Enzyme activities peaked during colonization but declined drastically during fruiting body formation. Highest activities achieved were: endoglucanase 12.3, exoglucanase 16.2, β-glucosidase 2.3, endoxylanase 20.3, amylase 0.26, laccase 14.8 and Mn-peroxidase 7.4 U/g dry substrate. © 2011 Springer Science+Business Media B.V.

Registro:

Documento: Artículo
Título:Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake)
Autor:Montoya, S.; Orrego, C.E.; Levin, L.
Filiación:Institute of Agricultural Biotechnology, Universidad de Caldas, Manizales, Colombia
Department of Physics and Chemistry, Universidad Nacional de Colombia sede Manizales, Manizales, Colombia
Biodiversity and Experimental Biology Department, PRHIDEB-CONICET, FCEN, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Coffee spent-ground; Grifola frondosa; Lignocellulolytic enzymes; Solid state fermentation; White-rot fungi; Activity profile; Biological efficiency; Cellulase activity; Corn bran; Dry substrates; Edible mushroom; Endoglucanases; Endoxylanase; Exoglucanase; Fruiting bodies; Glucosidase; Grifola frondosa; Laccases; Lignin contents; Lignocellulolytic enzymes; Lignocellulosic wastes; Medicinal fungus; Mushroom production; Oak sawdust; Organic recycling; Polysaccharide production; Qualitative assays; Solid-state fermentation; White rot fungi; Xylanases; Byproducts; Cellulose; Enzyme activity; Fermentation; Fungi; Lignin; Manganese; Substrates; cellulase; lignin; lignin peroxidase; lignocellulose; peroxidase; article; chemistry; culture medium; enzymology; Grifola; growth, development and aging; industrial waste; metabolism; Cellulases; Culture Media; Grifola; Industrial Waste; Lignin; Peroxidases; Agaricus bisporus; Basidiomycota; Fungi; Grifola frondosa; Zea mays
Año:2012
Volumen:28
Número:4
Página de inicio:1533
Página de fin:1541
DOI: http://dx.doi.org/10.1007/s11274-011-0957-2
Título revista:World Journal of Microbiology and Biotechnology
Título revista abreviado:World J. Microbiol. Biotechnol.
ISSN:09593993
CODEN:WJMBE
CAS:cellulase, 9012-54-8; lignin, 9005-53-2; lignin peroxidase, 42613-30-9; lignocellulose, 11132-73-3; peroxidase, 9003-99-0; Cellulases, 3.2.1.-; Culture Media; Industrial Waste; Lignin, 9005-53-2; Peroxidases, 1.11.1.-; lignin peroxidase, 1.11.1.-; lignocellulose, 11132-73-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v28_n4_p1533_Montoya

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Citas:

---------- APA ----------
Montoya, S., Orrego, C.E. & Levin, L. (2012) . Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake). World Journal of Microbiology and Biotechnology, 28(4), 1533-1541.
http://dx.doi.org/10.1007/s11274-011-0957-2
---------- CHICAGO ----------
Montoya, S., Orrego, C.E., Levin, L. "Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake)" . World Journal of Microbiology and Biotechnology 28, no. 4 (2012) : 1533-1541.
http://dx.doi.org/10.1007/s11274-011-0957-2
---------- MLA ----------
Montoya, S., Orrego, C.E., Levin, L. "Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake)" . World Journal of Microbiology and Biotechnology, vol. 28, no. 4, 2012, pp. 1533-1541.
http://dx.doi.org/10.1007/s11274-011-0957-2
---------- VANCOUVER ----------
Montoya, S., Orrego, C.E., Levin, L. Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake). World J. Microbiol. Biotechnol. 2012;28(4):1533-1541.
http://dx.doi.org/10.1007/s11274-011-0957-2