Artículo

Sabaté, D.C.; Gonzaléz, M.J.; Porrini, M.P.; Eguaras, M.J.; Audisio, M.C.; Marioli, J.M. "Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae" (2012) World Journal of Microbiology and Biotechnology. 28(4):1415-1422
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Abstract:

The aim of this work was to determine the in vitro effect of the mixture between the lipopeptide surfactin, synthesized by Bacillus subtilis C4 (strain isolated from honey) and the most active vegetal extract from Achyrocline satureioides, a traditional medicinal plant, on local strains of Paenibacillus larvae, the agent of American Foulbrood in honeybees. Five P. larvae strains isolated in Córdoba, Argentina, were phenotypically characterized. These and 12 other P. larvae strains from different regions of Argentina were analysed. The antimicrobial activities of the essential oil, hexane (HE) and benzene extracts from A. satureioides were assessed against P. larvae and the HE showed the highest anti-P. larvae activity. A combination of the biosurfactant surfactin, produced by B. subtilis C4, and the HE of A. satureioides revealed a synergistic action on P. larvae. The effective surfactin concentration in the mixture decreased from 32 to 1 μg ml-1 and the HE concentration from 32 to 4 μg ml-1, values similar or equal to minimal inhibitory concentrations observed for oxytetracycline. The fractional inhibitory concentration index confirmed synergism in 4 strains and partial synergism in one strain. The combination of surfactin synthesized by B. subtilis C4 and the HE from A. satureioides could be a natural alternative to help beekeepers to combat the American foulbrood agent P. larvae. © 2011 Springer Science+Business Media B.V.

Registro:

Documento: Artículo
Título:Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
Autor:Sabaté, D.C.; Gonzaléz, M.J.; Porrini, M.P.; Eguaras, M.J.; Audisio, M.C.; Marioli, J.M.
Filiación:Instituto de Investigaciones para la Industria Química (INIQUI), Salta, Argentina
Universidad Nacional de Salta, Avenida Bolivia 5150, A4408FVY Salta, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Salta, Argentina
Departamento de Microbiología e Inmunología;, Facultad de Ciencias Exactas, Fisicoquímicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina
Arthropods Laboratory FCEyN, Buenos Aires, Argentina
Universidad Nacional de Mar del Plata, Buenos Aires, Argentina
Departamento de Química, Facultad de Ciencias Exactas, Fisicoquímicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina
Palabras clave:Achyrocline satureioides; Antimicrobial; Paenibacillus larvae; Surfactin; Synergism; Achyrocline satureioides; Antimicrobial; Paenibacillus larvae; Surfactin; Synergism; Benzene; Hexane; Microorganisms; Plant extracts; Strain; Surface active agents; Essential oils; antiinfective agent; cyclopeptide; lipopeptide; plant extract; surfactin peptide; Achyrocline; Argentina; article; Bacillus subtilis; chemistry; drug effect; drug potentiation; isolation and purification; microbial sensitivity test; microbial viability; Paenibacillus; physiology; Achyrocline; Anti-Bacterial Agents; Argentina; Bacillus subtilis; Drug Synergism; Lipopeptides; Microbial Sensitivity Tests; Microbial Viability; Paenibacillus; Peptides, Cyclic; Plant Extracts; Achyrocline; Achyrocline satureioides; Apis mellifera; Bacillus subtilis; Paenibacillus; Paenibacillus larvae
Año:2012
Volumen:28
Número:4
Página de inicio:1415
Página de fin:1422
DOI: http://dx.doi.org/10.1007/s11274-011-0941-x
Título revista:World Journal of Microbiology and Biotechnology
Título revista abreviado:World J. Microbiol. Biotechnol.
ISSN:09593993
CODEN:WJMBE
CAS:Anti-Bacterial Agents; Lipopeptides; Peptides, Cyclic; Plant Extracts; surfactin peptide, 24730-31-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v28_n4_p1415_Sabate

Referencias:

  • Albo, G.N., Henning, C., Ringuelet, J.A., Reynaldi, F.J., de Giusti, M.R., Alippi, A.M., Evaluation of some essential oils for the control and prevention of American Foulbrood disease in honey bees (2003) Apidologie, 34, pp. 417-437
  • Alippi, A.M., Caracterización de aislamientos de Paenibacillus larvae mediante tipo bioquímico y resistencia a oxitetraciclina (1996) Rev Arg Microbiol, 28, pp. 197-203
  • Alippi, A.M., López, A.C., Reynaldi, F.J., Grasso, D.H., Aguilar, O.M., Evidence for plasmid-mediated tetracycline resistance in Paenibacillus larvae, the causal agent of American Foulbrood (AFB) disease in honeybees (2007) Vet Microbiol, 125, pp. 290-303
  • Antúnez, K., Harriet, J., Gende, L., Maggi, M., Eguaras, M., Zunino, P., Efficacy of natural propolis extract in the control of American Foulbrood (2008) Vet Microbiol, 131, pp. 324-331
  • Audisio, M.C., Terzolo, H.R., Apella, M.C., Bacteriocin from honeybee beebread Enterococcus avium active against Listeria monocytogenes (2005) Appl Environ Microbiol, 71, pp. 3373-3375
  • Audisio, M.C., Torres, M.J., Sabaté, D.C., Ibarguren, C., Apella, M.C., Properties of different lactic acid bacteria isolated from Apis mellifera L. bee-gut (2011) Microbiol Res, 166, pp. 1-13
  • Bagamboula, C.F., Uyttendaele, M., Debevere, J., Antimicrobial effect of spices and herbs on Shigella sonnei and Shigella flexneri (2003) J Food Prot, 66, pp. 668-673
  • Bambrick, J.F., Resistance to American foulbrood in honey bees V. Comparative pathogenesis in resistant and susceptible larvae (1964) J Insect Pathol, 6, pp. 284-304
  • Bambrick, J.F., Rothenbuhler, W.C., Resistance to American fouldbrood in honey bees. IV. The relationship between larvae age at inoculation and mortality in a resistant and in a susceptible line (1961) J Insect Pathol, 3, pp. 381-390
  • Bastos, E.M.A.F., Simone, M., Jorge, D.M., Soares, A.E.E., Spivak, M., In vitro study of the antimicrobial activity of Brazilian propolis against Paenibacillus larvae (2008) J Invertebr Pathol, 97, pp. 273-281
  • Broussalis, A.M., Ferraro, G.E., Gurni, A.A., Coussio, J.D., Aspectos fitoquímicos de especies Argentinas del género Achyrocline (1989) Acta Farm Bonaerense, 8, pp. 11-16
  • Carney, J.R., Krenisky, J.M., Williamson, R.T., Luo, J., Achyrofuran, a new antihyperglycemic dibenzofuran from the South American medicinal plant Achyrocline satureioides (2002) J Nat Prod, 62, pp. 203-205
  • de Souza, K.C.B., Schapoval, E.E.S., Bassani, V.L., LC determination of flavonoids: separation of quercetin, luteolin and 3-0-methylquercetin in Achyrocline satureioides preparations (2002) J Pharm Biomed, 28, pp. 771-777
  • de Vos, P., Ludwig, W., Schleifer, K.H., Whitman, W.B., Family IV. Paenibacillaceae fam. nov
  • Desai, J.D., Banat, I.M., Microbial production of surfactants and their commercial potential (1997) Microbiol Biol Rev, 61, pp. 47-64
  • Dorman, H.J.D., Deans, S.G., Antimicrobial agents from plants: antibacterial activity of plant volatile oils (2000) J Appl Microbiol, 88, pp. 308-316
  • Eliopoulos, G.M., Moellering, R.C., Antimicrobial combinations (1996) Antibiotics in laboratory medicine, pp. 330-396. , 4th edn. Williams and Wilkins, Baltimore
  • Evans, J.D., Diverse origins of tetracycline resistance in the honey bee bacterial pathogen Paenibacillus larvae (2003) J Invertebr Pathol, 83, pp. 46-50
  • Evans, J.D., Armstrong, T.N., Inhibition of the American foulbrood bacterium, Paenibacillus larvae, by bacteria isolated from honey bees (2005) J Apic Res, 44, pp. 168-171
  • Filot da silva, L., Langeloh, A., A comparative study of antispasmodic activity of hydroalcoholic 80% (v/v) extracts of Achyrocline satureioides (Larn.) DC. (Asteraceae) with papaverine and atropine on rat isolated jejunum (1994) Acta Farm Bonaerense, 13, pp. 35-40
  • Finola, M.S., Lasagno, M.C., Marioli, J.M., Microbiological and chemical characterization of honeys from central Argentina (2007) Food Chem, 100, pp. 1649-1653
  • Flesar, J., Havlik, J., Kloucek, P., Rada, V., Titera, D., Vendar, M., Stropnicky, M., Kokoska, L., In vitro growth-inhibitory effect of plant-derived extracts and compounds against Paenibacillus larvae and their acute oral toxicity to adult honey bees (2010) Vet Microbiol, 145, pp. 129-133
  • Geissman, T.A., (1962) The Chemistry of Flavonoid Compounds, pp. 107-155. , New York: Pergamon Press
  • Gende, L.B., Bailac, P., Maggi, M.D., Ponzi, M., Fritz, R., Eguaras, M.J., Antimicrobial activity of Pimpinella anisum and Foeniculum vulgare essential oils against Paenibacillus larvae (2009) J Essent Oil Res, 21, pp. 91-93
  • González, M.J., (2005) Actividad antimicrobiana de extractos y/o productos vegetales de plantas aromáticas contra Paenibacillus larvae subespecie larvae, , Tesis de grado para optar al título de Microbióloga. Universidad Nacional de Río Cuarto
  • González, M.J., Marioli, J.M., Antibacterial activity of water extracts and essential oils of various aromatic 3 plants against Paenibacillus larvae, the causative agent of American Foulbrood (2010) J Invertebr Pathol, 104, pp. 209-213
  • Hall, M.J., Middleton, R.F., Westmacott, D., The fraccional inhibitory concentration (FIC) index as a measure of synergy (1983) J Antimicrob Chemother, 11, pp. 427-433
  • Harbone, J.B., (1984) Phytochemical methods: A guide to modern technique of plant analysis, pp. 57-168. , 2° Edición. Chapman and Hall
  • Helander, I.M., Alakomi, H.L., Latva-Kala, K., Mattila-Sandholm, T., Pol, I., Smid, E.J., Gorris, L.G.M., von Wright, A., Characterization of the action of selected essential oil components on Gram-negative bacteria (1998) J Agric Food Chem, 46, pp. 3590-3595
  • Hemaiswarya, S., Kruthiventi, A.K., Doble, M., Synergism between natural products and antibiotics against diseases (2008) Phytomedicine, 15, pp. 639-652
  • Krogstad, D., Moellering, R.C., Antimicrobial combinations (1986) Antibiotics in laboratory medicine, pp. 557-578. , 2nd edn. Williams and Wilkins, Baltimore
  • Kumar, K.A., Mazumdar, K., Dutta, N.K., Karak, P., Dastidar, S.G., Ray, R., Evaluation of synergism between the aminoglycoside antibiotic streptomycin and the cardiovascular agent amlodipine (2004) Biol Pharm Bull, 27, pp. 1116-1120
  • Mann, C.M., Markham, J.L., A new method for determining the minimum inhibitory concentration of essential oils (1998) J Appl Microbiol, 84, pp. 538-544
  • Miyagi, T., Peng, C.Y.S., Chuang, R.Y., Mussen, E.C., Spivak, M.S., Doi, R.H., Verification of oxytetracycline-resistant American foulbrood pathogen Paenibacillus larvae in the United States (2000) J Invertebr Pathol, 75, pp. 95-96
  • Mutinelli, F., European legislation governing the authorization of veterinary medicinal products with particular reference to the use of drugs for the control of honey bee diseases (2003) Apiacta, 38, pp. 156-168
  • Porrini, M.P., Audisio, M.C., Sabaté, D.C., Ibarguren, C., Medici, S.K., Sarlo, E.G., Garrido, P.M., Eguaras, M.J., Effect of bacterial metabolites on microsporidian Nosema ceranae and on its host Apis mellifera (2010) Parasitol Res, 107, pp. 381-388
  • Reynaldi, F.J., Albo, G.N., Alippi, A.M., Effectiveness of tilmicosin against Paenibacillus larvae, the causal agent of American Foulbrood disease of honeybees (2008) Vet Microbiol, 132, pp. 119-128
  • Rivera, F., Gervaz, E., Sere, C., Dajas, F., Toxicological studies of the aqueous extract from Achyrocline satureioides (Lam.) DC (Marcela) (2004) J Ethnopharmacol, 95, pp. 359-362
  • Rodrigues, F.F., Costa, J.G., Coutinho, H.D., Synergy effects of the antibiotics gentamicin and the essential oil of Croton zehntneri (2009) Phytomedicine, 16, pp. 1052-1055
  • Sabaté, D.C., Carrillo, L., Audisio, M.C., Inhibition of Paenibacillus larvae and Ascosphaera apis by Bacillus subtilis isolated from honeybee gut and honey samples (2009) Res Microbiol, 160, pp. 163-169
  • Sivropoulou, A., Papanikolaou, E., Nikolanou, C., Kokkini, S., Lanaras, T., Arsenakis, M., Antimicrobial and cytotoxic activities of Origanum essential oils (1996) J Agric Food Chem, 44, pp. 1202-1205
  • Vázquez, F., de los Santos, M., Grometbauer, C., (2009) Informe Apícola. Síntesis Apícola, , http://www.alimentosargentinos.gov.ar/0-/apicola/01_info/a_sintesis/lista/148.pdf, SAGPyA. Available at
  • Wagner, H., Ulrich-Merzenich, G., Synergy research: approaching a new generation of phytopharmaceuticals (2009) Phytomedicine, 16, pp. 97-110
  • White, R.L., Burgess, D.S., Manduru, M., Bosso, J.A., Comparison of three different in vitro methods of detecting synergy: time-kill, checkerboard and E-test (1996) Antimicrob Agents Chemother, 40, pp. 1914-1918
  • Williams, D.L., A veterinary approach to the European honey bee (Apis mellifera) (2000) Vet J, 160, pp. 61-73
  • Yoshiyama, M., Kimura, K., Bacteria in the gut of Japanese honeybee, Apis cerana japonica, and their antagonistic effect against Paenibacillus larvae, the causal agent of American foulbrood (2009) J Invertebr Pathol, 102, pp. 91-96
  • Youssef, N.H., Duncan, K.E., Nagle, D.P., Savage, K.N., Knapp, R.M., McInerney, M.J., Comparison of methods to detect biosurfactant production by diverse microorganisms (2004) J Microbiol Meth, 56, pp. 339-347
  • Zaika, L.L., Spices and herbs: their antibacterial activity and its determination (1988) J Food Saf, 23, pp. 97-118

Citas:

---------- APA ----------
Sabaté, D.C., Gonzaléz, M.J., Porrini, M.P., Eguaras, M.J., Audisio, M.C. & Marioli, J.M. (2012) . Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae. World Journal of Microbiology and Biotechnology, 28(4), 1415-1422.
http://dx.doi.org/10.1007/s11274-011-0941-x
---------- CHICAGO ----------
Sabaté, D.C., Gonzaléz, M.J., Porrini, M.P., Eguaras, M.J., Audisio, M.C., Marioli, J.M. "Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae" . World Journal of Microbiology and Biotechnology 28, no. 4 (2012) : 1415-1422.
http://dx.doi.org/10.1007/s11274-011-0941-x
---------- MLA ----------
Sabaté, D.C., Gonzaléz, M.J., Porrini, M.P., Eguaras, M.J., Audisio, M.C., Marioli, J.M. "Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae" . World Journal of Microbiology and Biotechnology, vol. 28, no. 4, 2012, pp. 1415-1422.
http://dx.doi.org/10.1007/s11274-011-0941-x
---------- VANCOUVER ----------
Sabaté, D.C., Gonzaléz, M.J., Porrini, M.P., Eguaras, M.J., Audisio, M.C., Marioli, J.M. Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae. World J. Microbiol. Biotechnol. 2012;28(4):1415-1422.
http://dx.doi.org/10.1007/s11274-011-0941-x