Artículo
Silvani, V.A.; Rothen, C.P.; Rodríguez, M.A.; Godeas, A.; Fracchia, S. "The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro" (2012) World Journal of Microbiology and Biotechnology. 28(12):3393-3397
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Abstract:
In the present study, we obtained in vitro dual cultures between the liverwort Plagiochasma rupestre and two arbuscular mycorrhizal (AM) fungi: Glomus intraradices and Glomus clarum. Four agarized culture media were tested for optimal growth of P. rupestre. Also, a description of the symbiotic association is provided. Plagiochasma rupestre gametophytes profusely grew axenically in MM with sucrose, and thalli were successfully subcultured under these growth conditions. Arbuscular mycorrhizal fungal hyphae colonized P. rupestre thalli through rhizoids or by forming appresoria in the ventral thallus cells. Arbuscules, mycelia and structures resembling intrathallic spores or vesicles were developed in the internal parenchymatic cells. The pattern of AM colonization in P. rupestre was very similar to the Paris-type. After 100 days of dual culture, the external mycelia of both AM fungal strains formed thousands of small viable spores, suggesting that P. rupestre in vitro culture could be a valuable tool for studying the biology of both symbiotic partners and conserving AM fungi in in vitro germplasm collections. © 2012 Springer Science+Business Media B.V.
Registro:
| Documento: | Artículo |
| Título: | The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro |
| Autor: | Silvani, V.A.; Rothen, C.P.; Rodríguez, M.A.; Godeas, A.; Fracchia, S. |
| Filiación: | Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 4to piso Pabellón 2, 1428 Buenos Aires, Argentina Centro Regional de Investigaciones Científicas y Transferencia Tecnológica, CRILAR- CONICET Entre Ríos y Mendoza, 5301 Anillaco, La Rioja, Argentina |
| Palabras clave: | Arbuscular mycorrhiza; Glomus; Liverworts; Monoxenic culture; Plagiochasma rupestre; Am fungus; Arbuscular mycorrhizal; Arbuscular mycorrhizal fungi; Arbuscular mycorrhizas; Culture media; Fungal hyphae; Fungal strains; Germplasm collections; Glomus; Glomus intraradices; Growth conditions; In-vitro; Liverworts; Optimal growth; Plagiochasma rupestre; Symbiotic association; Symbiotic partners; Fungi; Sugar (sucrose); Cell culture; article; chemistry; culture medium; cytology; fungus spore; Glomeromycota; growth, development and aging; liverwort; microbiology; mycelium; mycorrhiza; physiology; symbiosis; Culture Media; Glomeromycota; Hepatophyta; Mycelium; Mycorrhizae; Spores, Fungal; Symbiosis; Arbuscular; Bacteria (microorganisms); Fungi; Glomus; Glomus clarum; Glomus intraradices; Marchantiophyta; Plagiochasma rupestre |
| Año: | 2012 |
| Volumen: | 28 |
| Número: | 12 |
| Página de inicio: | 3393 |
| Página de fin: | 3397 |
| DOI: | http://dx.doi.org/10.1007/s11274-012-1146-7 |
| Título revista: | World Journal of Microbiology and Biotechnology |
| Título revista abreviado: | World J. Microbiol. Biotechnol. |
| ISSN: | 09593993 |
| CODEN: | WJMBE |
| CAS: | Culture Media |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v28_n12_p3393_Silvani |
Referencias:
- Bardón, A., Kamiya, N., Toyota, M., Takaoka, S., Asakawa, Y., Sesquiterpenoids, hopanoids and bis(bibenzyls) from the Argentine liverwort Plagiochasma rupestre (1999) Phytochemistry, 52, pp. 1323-1329
- Bidartondo, M.I., Read, D.J., Trappe, J.M., Merckx, V., Ligrone, R., Duckett, J.G., The dawn of symbiosis between plants and fungi (2011) Biol Lett, 7, pp. 574-577
- Boullard, B., Observations of the coevolution of fungi with hepatics (1988) Coevolution of Fungi with Plants and Animals, pp. 107-124. , K. A. Pirozynski and D. L. Hawksworth (Eds.), London: Academic Press
- Brundrett, M.C., Coevolution of roots and mycorrhizas of land plants (2002) New Phytol, 154, pp. 275-304
- Carafa, A., Duckett, J.G., Ligrone, R., Subterranean gametophytic axes in the primitive liverwort Haplomitrium harbour a unique type of endophytic association with aseptate fungi (2003) New Phytol, 160, pp. 185-197
- Duckett, J.G., Carafa, A., Ligrone, R., A highly differentiated glomeromycotean association with the mucilage-secreting, primitive antipodean liverwort Treubia: clues to the origins of mycorrhizas (2006) Am J Bot, 93, pp. 797-813
- Evans, A.W., The genus Plagiochasma and its North American species (1915) Bull Torrey Bot Club, 42 (5), pp. 259-308
- Fonseca, H.M.A.C., Berbara, R.L.L., Does Lunularia cruciata form symbiotic relationships with either Glomus proliferum or G. intraradices? (2008) Mycol Res, 112, pp. 1063-1068
- Fonseca, H.M.A.C., Berbara, R.L.L., Pereira, M.L., Lunularia cruciata a potential in vitro host for Glomus proliferum and G. intraradices (2006) Mycorrhiza, 16, pp. 503-508
- Ligrone, R., Carafa, A., Lumini, E., Bianciotto, V., Bonfante, P., Duckett, J.G., Glomeromycotean associations in liverworts: a molecular, cellular and taxonomic analysis (2007) Am J Bot, 94, pp. 1756-1777
- Marsh, B.A.B., Measurement of length in random arrangements of lines (1971) J Appl Ecol, 8, pp. 265-270
- Pressel, S., Bidartondo, M.I., Ligrone, R., Duckett, J.G., Fungal symbioses in bryophytes: new insights in the twenty first century (2010) Phytotaxa, 9, pp. 238-253
- Read, D.J., Duckett, J.G., Francis, R., Ligrone, R., Russell, A., Symbiotic fungal associations in 'lower' land plants (2000) Phil Trans R Soc Lond B Biol Sci, 355, pp. 815-830
- Russell, J., Bulman, S., The liverwort Marchantia foliacea forms a specialized symbiosis with arbuscular mycorrhizal fungi in the genus Glomus (2005) New Phytol, 165, pp. 567-579
- Schüßler, A., Glomus claroideum forms an arbuscular mycorrhiza-like symbiosis with the hornwort Anthoceros punctatus (2000) Mycorrhiza, 10, pp. 15-21
- Silvani, V.A., Fracchia, S., Fernández, L., Pergola, M., Godeas, A., A simple method to obtain endophytic microorganisms from field-collected roots (2008) Soil Biol Biochem, 40, pp. 1259-1263
- St-Arnaud, M., Hamel, C., Vimard, B., Caron, M., Fortin, J.A., Enhanced hyphal growth and spore production of the arbuscular mycorrhizal fungus Glomus intraradices in an in vitro system in the absence of host roots (1996) Mycol Res, 100, pp. 328-332
- Strack, D., Fester, T., Isoprenoid metabolism and plastid reorganization in arbuscular mycorrhizal roots (2006) New Phytol, 172, pp. 22-34
Citas:
---------- APA ----------
Silvani, V.A., Rothen, C.P., Rodríguez, M.A., Godeas, A. & Fracchia, S. (2012) . The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro. World Journal of Microbiology and Biotechnology, 28(12), 3393-3397.http://dx.doi.org/10.1007/s11274-012-1146-7
---------- CHICAGO ----------
Silvani, V.A., Rothen, C.P., Rodríguez, M.A., Godeas, A., Fracchia, S. "The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro" . World Journal of Microbiology and Biotechnology 28, no. 12 (2012) : 3393-3397.http://dx.doi.org/10.1007/s11274-012-1146-7
---------- MLA ----------
Silvani, V.A., Rothen, C.P., Rodríguez, M.A., Godeas, A., Fracchia, S. "The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro" . World Journal of Microbiology and Biotechnology, vol. 28, no. 12, 2012, pp. 3393-3397.http://dx.doi.org/10.1007/s11274-012-1146-7
---------- VANCOUVER ----------
Silvani, V.A., Rothen, C.P., Rodríguez, M.A., Godeas, A., Fracchia, S. The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro. World J. Microbiol. Biotechnol. 2012;28(12):3393-3397.http://dx.doi.org/10.1007/s11274-012-1146-7
