Artículo

Fernández, L.; Silvani, V.; Bompadre, J.; Pérgola, M.; Godeas, A. "Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis" (2009) World Journal of Microbiology and Biotechnology. 25(10):1857-1863
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Abstract:

Ri T-DNA transformed roots have been used effectively in studying the interaction between various plant hosts and arbuscular mycorrhizal (AM) fungi. We investigated the in vitro monoxenic symbiosis between the AM fungus Glomus intraradices and transformed soybean roots (TSRs). Comparisons were made between TSR system and plants of the same genotype. The extraradical fungal structures generated in vitro culture showed normal development. Straight runner hyphae branched into short simple branched absorbing structures and spores were initiated. AM symbiosis was confirmed by the presence of arbuscules and vesicles in cortical cells of the TSRs. The frequency of intraradical colonization in TSRs was higher than in plants grown in soil, whereas the intensity values of intraradical colonization in TSR cultures were similar to those in whole plants. These results show that TSR cultures were able to support the growth and characteristic development of G. intraradices. © 2009 Springer Science+Business Media B.V.

Registro:

Documento: Artículo
Título:Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis
Autor:Fernández, L.; Silvani, V.; Bompadre, J.; Pérgola, M.; Godeas, A.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 4to piso Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Glomus intraradices; Monoxenic culture; Transformed soybean roots; Absorbing structure; Am fungus; Arbuscular mycorrhizal fungi; Arbuscular mycorrhizal symbiosis; Characteristic development; Cortical cells; Glomus intraradices; Glycine max; In-plants; In-vitro; Intensity values; Plant hosts; Soybean roots; Whole plants; Amplitude modulation; Nitrogen fixation; Nucleic acids; Plants (botany); Cell culture; Arbuscular; Bacteria (microorganisms); Fungi; Glomus intraradices; Glycine max
Año:2009
Volumen:25
Número:10
Página de inicio:1857
Página de fin:1863
DOI: http://dx.doi.org/10.1007/s11274-009-0065-8
Título revista:World Journal of Microbiology and Biotechnology
Título revista abreviado:World J. Microbiol. Biotechnol.
ISSN:09593993
CODEN:WJMBE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v25_n10_p1857_Fernandez

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Citas:

---------- APA ----------
Fernández, L., Silvani, V., Bompadre, J., Pérgola, M. & Godeas, A. (2009) . Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis. World Journal of Microbiology and Biotechnology, 25(10), 1857-1863.
http://dx.doi.org/10.1007/s11274-009-0065-8
---------- CHICAGO ----------
Fernández, L., Silvani, V., Bompadre, J., Pérgola, M., Godeas, A. "Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis" . World Journal of Microbiology and Biotechnology 25, no. 10 (2009) : 1857-1863.
http://dx.doi.org/10.1007/s11274-009-0065-8
---------- MLA ----------
Fernández, L., Silvani, V., Bompadre, J., Pérgola, M., Godeas, A. "Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis" . World Journal of Microbiology and Biotechnology, vol. 25, no. 10, 2009, pp. 1857-1863.
http://dx.doi.org/10.1007/s11274-009-0065-8
---------- VANCOUVER ----------
Fernández, L., Silvani, V., Bompadre, J., Pérgola, M., Godeas, A. Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis. World J. Microbiol. Biotechnol. 2009;25(10):1857-1863.
http://dx.doi.org/10.1007/s11274-009-0065-8