Abstract:
Biosorption of heavy metals by gram-positive, non-pathogenic and non-toxicogenic Paenibacillus polymyxa P13 was evaluated. Copper was chosen as a model element because it is a pollutant originated from several industries. An EPS (exopolysaccharide)-producing phenotype exhibited significant Cu(II) biosorption capacity. Under optimal assay conditions (pH 6 and 25°C), the adsorption isotherm for Cu(II) in aqueous solutions obeyed the Langmuir model. A high q value (biosorption capacity) was observed with whole cells (q max=112 mgCu g-1). EPS production was associated with hyperosmotic stress by high salt (1 M NaCl), which led to a significant increase in the biosorption capacity of whole cells (q max=150 mgCu g -1). Biosorption capacity for Cu(II) of the purified EPS was investigated. The maximum biosorption value (q) of 1602 mg g-1 observed with purified EPS at 0.1 mg ml-1 was particularly promising for use in field applications. © Springer 2005.
Registro:
Documento: |
Artículo
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Título: | Biosorption of copper by Paenibacillus polymyxa cells and their exopolysaccharide |
Autor: | Prado Acosta, M.; Valdman, E.; Leite, S.G.F.; Battaglini, F.; Ruzal, S.M. |
Filiación: | Dto. Química Biológica, FCEN-Universidad de Buenos Aires, Argentina Escola de Química, Universidade Federal Do Rio de Janeiro, Brazil DQIAyQF, FCEN - Universidad de Buenos Aires, Argentina
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Palabras clave: | Biosorption; Exopolysaccharide; Heavy metal; Paenibacillus polymyxa; Cells; Heavy metals; Microbiology; Pollution; Sorption; Biosorption; Langmuir models; Biotechnology; Paenibacillus polymyxa; Posibacteria |
Año: | 2005
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Volumen: | 21
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Número: | 6-7
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Página de inicio: | 1157
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Página de fin: | 1163
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DOI: |
http://dx.doi.org/10.1007/s11274-005-0381-6 |
Título revista: | World Journal of Microbiology and Biotechnology
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Título revista abreviado: | World J. Microbiol. Biotechnol.
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ISSN: | 09593993
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CODEN: | WJMBE
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v21_n6-7_p1157_PradoAcosta |
Referencias:
- Brenner, A.J., Harris, E.D., A quantitative test for copper using bicinchoninic acid (1995) Analalytical Biochemistry, 226, pp. 80-84. , Erratum in: Analytical Biochemistry (1995) 230, 360
- Gutnick, D.L., Bach, H., Engineering bacterial biopolymers for the biosorption of heavy metals; New products and novel formulations (2000) Applied Microbiology and Biotechnology, 54, pp. 451-60
- Ince Yilmaz, E., Metal tolerance and biosorption capacity of Bacillus circulans strain EB1 (2003) Research in Microbiology, 154, pp. 409-415
- Jackson, P., High-Resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides (1994) Guide to Techniques in Glycobiology. Methods in Enzymology, 230, pp. 250-265
- Kachlany, S.C., Levery, S.B., Kim, J.S., Reuhs, B.L., Lion, L.W., Ghiorse, W.C., Structure and carbohydrate analysis of the exopolysaccharide capsule of Pseudomonas putida G7 (2001) Environmental Microbiology, 3, pp. 774-784
- Kielland, J., Individual activities coefficients of ions in aqueous solutions (1937) Journal of the American Chemical Society, 59, pp. 1675-1678
- Kim, S.Y., Kim, J.H., Kim, C.I., Oh, O.K., Metal adsorption of the polysaccharide produced from Methylobacterium organophilum (1996) Biotechnology Letters, 18, pp. 1161-1164
- Loaec, M., Olier, R., Guezennec, J., (1997) Uptake of Lead, Cadmium and Zinc by a Novel Bacterial Exopolysaccharide Water Research, 31, pp. 1171-1179
- Ozdemir, G., Ozturk, T., Ceyhan, N., Isler, R., Cosar, T., Heavy metal biosorption by biomass of Ochrobactrum anthropi producing exopolysaccharide in activated sludge (2003) Bioresource Technology, 90, pp. 71-74
- Penaloza-Vazquez, A., Kidambi, S.P., Chakrabarty, A.M., Bender, C.L., Characterization of the alginate biosynthetic gene cluster in Pseudomonas syringae pv. syringae (1997) Journal of Bacteriology, 179, pp. 4464-4472
- Piuri, M., Sanchez-Rivas, C., Ruzal, S.M., A novel antimicrobial activity of a Paenibacillus polymyxa strain isolated from regional fermented sausages (1998) Letters in Applied Microbiology, 27, pp. 9-13
- Ricciardi, A., Parente, E., Crudele, M.A., Zanetti, F., Scolari, G., Mannazzu, I., Exopolysaccharide production by Streptococcus thermophilus SY: Production and preliminary characterization of the polymer (2002) Journal of Applied Microbiology, 92, pp. 297-306
- Ruzal, S.M., Lopez, C., Rivas, E., Sanchez-Rivas, C., Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis (1998) Current Microbiology, 36, pp. 75-79
- Salehizadeh, H., Shojaosadati, S.A., Removal of metal ions from aqueous solution by polysaccharide produced from Bacillus firmus (2003) Water Research, 37, pp. 4231-4235
- Savvaidis, I., Hughes, M.N., Poole, R.K., Copper biosorption by Pseudomonas cepacia and others strains (2003) World Journal of Microbiology and Biotechnology, 19, pp. 117-121
- Singh, P., Cameotra, S.S., Enhancement of metal bioremediation by use of microbial surfactants (2004) Biochemical and Biophysical Research Communications, 319, pp. 291-297
- Valdman, E., Leite, S.G.F., Biosorption of Cd, Zn and Cu by Sargassum sp waste biomass (2000) Bioprocess Engineering, 2, pp. 171-173
- Volesky, B., Holan, Z.R., Biosorption of heavy metals (1995) Biotechnology Progress, 11, pp. 235-250
- Young, K.D., A simple gel electrophoretic method for analyzing the muropeptide composition of bacterial peptidoglycan (1996) Journal of Bacteriology, 178, pp. 3962-3966
Citas:
---------- APA ----------
Prado Acosta, M., Valdman, E., Leite, S.G.F., Battaglini, F. & Ruzal, S.M.
(2005)
. Biosorption of copper by Paenibacillus polymyxa cells and their exopolysaccharide. World Journal of Microbiology and Biotechnology, 21(6-7), 1157-1163.
http://dx.doi.org/10.1007/s11274-005-0381-6---------- CHICAGO ----------
Prado Acosta, M., Valdman, E., Leite, S.G.F., Battaglini, F., Ruzal, S.M.
"Biosorption of copper by Paenibacillus polymyxa cells and their exopolysaccharide"
. World Journal of Microbiology and Biotechnology 21, no. 6-7
(2005) : 1157-1163.
http://dx.doi.org/10.1007/s11274-005-0381-6---------- MLA ----------
Prado Acosta, M., Valdman, E., Leite, S.G.F., Battaglini, F., Ruzal, S.M.
"Biosorption of copper by Paenibacillus polymyxa cells and their exopolysaccharide"
. World Journal of Microbiology and Biotechnology, vol. 21, no. 6-7, 2005, pp. 1157-1163.
http://dx.doi.org/10.1007/s11274-005-0381-6---------- VANCOUVER ----------
Prado Acosta, M., Valdman, E., Leite, S.G.F., Battaglini, F., Ruzal, S.M. Biosorption of copper by Paenibacillus polymyxa cells and their exopolysaccharide. World J. Microbiol. Biotechnol. 2005;21(6-7):1157-1163.
http://dx.doi.org/10.1007/s11274-005-0381-6