Abstract:
A search for bioactive compounds, inhibitors of Paenibacillus larvae, the causal agent of American foulbrood, a honeybees' disease, was carried on. Extracts of two fungal strains, Alternaria brassicicola and Alternaria raphani, isolated from pollen collected from beehives, exhibited a specific inhibitory activity against this bacterium. From these extracts and by means of chromatographic steps and bioassay-guided fractionation, three tetramic acids were isolated. The compounds were identified by spectroscopic methods and the absolute stereochemistry was chemically determined. L-Tenuazonic acid was shown to be responsible for the antibiotic activity. This compound showed a MIC of 32 μg/ml, comparable with that of oxytetracycline, an antibiotic currently used for the prevention of American foulbrood.
Registro:
Documento: |
Artículo
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Título: | L-Tenuazonic acid, a new inhibitor of Paenibacillus Larvae |
Autor: | Gallardo, G.L.; Peña, N.I.; Chacana, P.; Terzolo, H.R.; Cabrera, G.M. |
Filiación: | Depto de Quím. Orgán., FCEyN, UBA, Cd. Univ., Pab. II Buenos A., Argentina Depto de Biología, FCEyN, UNMdP, Mar del Plata, Argentina, Argentina Lab. Bacteriología, Depto Prod. Animal, INTA EEA Balcarce, Argentina, Argentina
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Palabras clave: | Alternaria brassicicola; Alternaria raphani; American foulbrood; L-Tenuazonic acid; Paenibacillus larvae; tetramic acids; Alternaria; Alternaria brassicicola; Alternaria japonica; Apis mellifera; Bacteria (microorganisms); Paenibacillus; Paenibacillus larvae |
Año: | 2004
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Volumen: | 20
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Número: | 6
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Página de inicio: | 609
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Página de fin: | 612
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DOI: |
http://dx.doi.org/10.1023/B:WIBI.0000043175.23621.8c |
Título revista: | World Journal of Microbiology and Biotechnology
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Título revista abreviado: | World J. Microbiol. Biotechnol.
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ISSN: | 09593993
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CODEN: | WJMBE
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v20_n6_p609_Gallardo |
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Citas:
---------- APA ----------
Gallardo, G.L., Peña, N.I., Chacana, P., Terzolo, H.R. & Cabrera, G.M.
(2004)
. L-Tenuazonic acid, a new inhibitor of Paenibacillus Larvae. World Journal of Microbiology and Biotechnology, 20(6), 609-612.
http://dx.doi.org/10.1023/B:WIBI.0000043175.23621.8c---------- CHICAGO ----------
Gallardo, G.L., Peña, N.I., Chacana, P., Terzolo, H.R., Cabrera, G.M.
"L-Tenuazonic acid, a new inhibitor of Paenibacillus Larvae"
. World Journal of Microbiology and Biotechnology 20, no. 6
(2004) : 609-612.
http://dx.doi.org/10.1023/B:WIBI.0000043175.23621.8c---------- MLA ----------
Gallardo, G.L., Peña, N.I., Chacana, P., Terzolo, H.R., Cabrera, G.M.
"L-Tenuazonic acid, a new inhibitor of Paenibacillus Larvae"
. World Journal of Microbiology and Biotechnology, vol. 20, no. 6, 2004, pp. 609-612.
http://dx.doi.org/10.1023/B:WIBI.0000043175.23621.8c---------- VANCOUVER ----------
Gallardo, G.L., Peña, N.I., Chacana, P., Terzolo, H.R., Cabrera, G.M. L-Tenuazonic acid, a new inhibitor of Paenibacillus Larvae. World J. Microbiol. Biotechnol. 2004;20(6):609-612.
http://dx.doi.org/10.1023/B:WIBI.0000043175.23621.8c