Artículo

De Cano, M.S.; Zaccaro, M.C.; García, I.; Stella, A.M.; De Caire, G.Z. "Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)" (2003) World Journal of Microbiology and Biotechnology. 19(1):29-34
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Abstract:

Cyanobacteria produce a wide array of substances with activity in many biological systems. The aim of the present research was to compare the effect of differently treated extracellular products (EP) from Tolypothrix tenuis (Cyanobacteria) on rice plantlet regeneration as well as on the pigments, protein, total free porphyrin contents, and 5-aminolevulinate dehydratase (ALA-D) activity in rice callus during differentiation. Rice embryo calli were regenerated in Murashige & Skoog medium supplemented with EP with protein (EPp) or without protein (EPnp) or autoclaved (EPa), as well as with benzyladenine (BA) and benzyladenine + naphthaleneacetic acid (BA + NAA). At day 75, calli percentage regeneration were: EPnp (84%), EPp (58%), BA + NAA (45%), BA (44%), EPa (40%). The same trend was found for chlorophyll a, b, total and carotenoid contents. Protein content in BA, BA + NAA and EPnp treatments was 35% higher than in EPp and EPa. Total free porphyrin was similar in all treatments. ALA-D activity in BA, EPp and EPa treatments was 28% higher than in BA + NAA and EPnp. The extracellular bioactive substance(s) from T. tenuis would contain a mixture of thermolabile plant growth regulators that replaced and improved the effects of synthetic plant growth regulators on rice callus organogenesis. Calli were rhizogenic in all the regeneration media tested. The pigment content of the calli was related to percentage regeneration but not to the total free porphyrin and ALA-D activity.

Registro:

Documento: Artículo
Título:Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
Autor:De Cano, M.S.; Zaccaro, M.C.; García, I.; Stella, A.M.; De Caire, G.Z.
Filiación:Lab. Fisiol. Veg. Biol. C., Depto. de Ciencias Biológicas, FCEN Universidad de Buenos Aires, Intendente Güiraldes 2620, Buenos Aires 1428, Argentina
Consejo Nac. Invest. Cie. T., Depto. de Quimica Biologica, FCEN Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Bioactive extracellular substances; Cyanobacteria; Plant growth regulators; Plant regeneration; Porphyrin; Rice callus; Tolypothrix tenuis; Calothrix sp. PCC 7101; Cyanobacteria; Tolypothrix
Año:2003
Volumen:19
Número:1
Página de inicio:29
Página de fin:34
DOI: http://dx.doi.org/10.1023/A:1022574107580
Título revista:World Journal of Microbiology and Biotechnology
Título revista abreviado:World J. Microbiol. Biotechnol.
ISSN:09593993
CODEN:WJMBE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v19_n1_p29_DeCano

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Citas:

---------- APA ----------
De Cano, M.S., Zaccaro, M.C., García, I., Stella, A.M. & De Caire, G.Z. (2003) . Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria). World Journal of Microbiology and Biotechnology, 19(1), 29-34.
http://dx.doi.org/10.1023/A:1022574107580
---------- CHICAGO ----------
De Cano, M.S., Zaccaro, M.C., García, I., Stella, A.M., De Caire, G.Z. "Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)" . World Journal of Microbiology and Biotechnology 19, no. 1 (2003) : 29-34.
http://dx.doi.org/10.1023/A:1022574107580
---------- MLA ----------
De Cano, M.S., Zaccaro, M.C., García, I., Stella, A.M., De Caire, G.Z. "Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)" . World Journal of Microbiology and Biotechnology, vol. 19, no. 1, 2003, pp. 29-34.
http://dx.doi.org/10.1023/A:1022574107580
---------- VANCOUVER ----------
De Cano, M.S., Zaccaro, M.C., García, I., Stella, A.M., De Caire, G.Z. Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria). World J. Microbiol. Biotechnol. 2003;19(1):29-34.
http://dx.doi.org/10.1023/A:1022574107580