Navegar

Colección

Datos Estadísticas

Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Bacillus megaterium is a potential bioremediation and biocontrol agent. The accumulation of reserve polymers, such as poly-3-hydroxybutyrate (PHB), increases survival of B. megaterium in water. We used wild-type strains of this species and mutant strains deficient in PHB synthesis in soil microcosms for testing the hypothesis that differences in survival capabilities and spore quality between strains is maintained in heterogeneous environments enriched with organic matter. No differences in survival between strains, nor a decrease in bacterial cell numbers were observed in sterile soil microcosms. In non-sterile soil, the total cell number (vegetative cells plus spores) of the PHB wild-type strain was 3.5 times higher than that of the PHB-negative mutant. We suggest that for predictive purposes, validation of survival in a variety of conditions is necessary.

Registro:

Documento: Artículo
Título:Survival of poly-3-hydroxybutyrate-producing bacteria in soil microcosms
Autor:López, N.I.; Ruiz, J.A.; Méndez, B.S.
Filiación:Depto. de Ciencias Biológicas, Ciudad Universitaria, 1428-Buenos Aires, Argentina
Depto. de Quim. Biológica, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428-Buenos Aires, Argentina
Palabras clave:Bacillus megaterium; Bacterial survival; Microcosms; Poly-3-hydroxybutyrate; Soil; bacterial metabolism; bioaccumulation; biocontrol agent; bioremediation; poly(3 hydroxybutyric acid); soil microcosm; strain; survival; Bacillus megaterium
Año:1998
Volumen:14
Número:5
Página de inicio:681
Página de fin:684
DOI: http://dx.doi.org/10.1023/A:1008857420434
Título revista:World Journal of Microbiology and Biotechnology
Título revista abreviado:World J. Microbiol. Biotechnol.
ISSN:09593993
CODEN:WJMBE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v14_n5_p681_Lopez

Referencias:

  • Bertagnolli, B.L., Dalsoglio, F.K., Sinclair, J.B., Extracellular enzyme profiles of the fungal pathogen Rhizoctonia solani isolate 2B-12 and of two antagonists Bacillus megaterium strain b153-2-2 and Trichoderma harzianum isolate Th008. 1. Possible correlations with inhibition of growth and biocontrol (1996) Physiological and Molecular Plant Pathology, 48 (3), pp. 145-160
  • Bohall Jr., N.A., Vary, P.S., Transposition of Tn917 in Bacillus megaterium (1986) Journal of Bacteriology, 167, pp. 716-718
  • Clewell, D.B., Gawron-Burke, C., Conjugative transposons and the dissemination of antibiotic resistance in streptococci (1988) Annual Review of Microbiology, 40, pp. 635-665
  • Floccari, M., Tiscornia, G., Palmada, F., Méndez, B., Enriquecimiento en mutantes de Bacillus megaterium deficientes en la síntesis de poli(3-hidroxibutirato) (1991) Revista Argentina de Microbiología, 23, pp. 14-25
  • Lafuente, R., Maym-Gatel, X., Mas-Castellá, J., Guerrero, R., Influence of Environmental Factors on Plasmid Transfer in Soil Microcosms (1996) Current Microbiology, 32, pp. 213-220
  • López, N.I., Floccari, M.E., Steinbüchel, A., Garcia, A.F., Méndez, B.S., Effect of poly(3-hydroxybutyrate) (PHB) content on the starvation-survival of bacteria in natural waters (1995) FEMS Microbiology Ecology, 16, pp. 95-102
  • López, N.I., Duarte, C.M., Vallespinós, F., Romero, J., Alcoverro, T., Bacterial activities in seagrass (Posidonia oceanica) sediments (1995) Journal of Experimental Marine Biology and Ecology, 187, pp. 39-49
  • Ostle, A., Holt, J.G., Nile Blue a as a fluorescent stain for poly-hydroxybutyrate (1982) Applied and Environmental Microbiology, 44 (1), pp. 238-241
  • Postma, J., Van Veen, J.A., Walter, S., Influence of different initial soil moisture contents on the distribution and population dynamics on introduced Rhizobium leguminosarum biovar trifolii (1989) Soil Biology and Biochemistry, 21, pp. 437-442
  • Postma, J., Hok-A-Hin, C.H., Schotman, J.M.T., Wijffelman, C.A., Van Veen, J.A., Population dynamics of Rhizobium leguminosarum Tn5 mutants with altered cell surface properties introduced into sterile and nonsterile soils (1991) Applied and Environmental Microbiology, 57, pp. 649-654
  • Quinn, J.P., Pedens, J.M.M., Dick, R.E., Carbon-phosphorus bond cleavage by Gram-positive and Gram-negative soil bacteria (1984) Applied Microbiology and Biotechnology, 31, pp. 283-287
  • Saxena, A., Zhang, R.W., Bollag, J.M., Microorganisms capable of metabolizing the herbicide metolachlor (1987) Applied and Environmental Microbiology, 53, pp. 390-396
  • Tao, Y.-P., Vary, P.S., Isolation and characterization of a sporulation lacZ fusion mutants of Bacillus megaterium (1991) Journal of General Microbiology, 137, pp. 797-806
  • Van Elsas, J.D., Van Overbeek, L.S., Bacterial responses to soil stimuli (1992) Starvation in Bacteria, pp. 55-79. , ed Kjelleberg, S. New York: Plenum Press
  • Vary, P., Prime time for Bacillus megaterium (1994) Microbiology, 140, pp. 1001-1013
  • Wagner-Döbler, I., Pipke, R., Timmis, K.N., Dwyer, D.F., Evaluation of aquatic sediment microcosms and their use in assessing possible effects of introduced microorganisms on ecosystem parameters (1992) Applied and Environmental Microbiology, 58 (4), pp. 1249-1258

Citas:

---------- APA ----------
López, N.I., Ruiz, J.A. & Méndez, B.S. (1998) . Survival of poly-3-hydroxybutyrate-producing bacteria in soil microcosms. World Journal of Microbiology and Biotechnology, 14(5), 681-684.
http://dx.doi.org/10.1023/A:1008857420434
---------- CHICAGO ----------
López, N.I., Ruiz, J.A., Méndez, B.S. "Survival of poly-3-hydroxybutyrate-producing bacteria in soil microcosms" . World Journal of Microbiology and Biotechnology 14, no. 5 (1998) : 681-684.
http://dx.doi.org/10.1023/A:1008857420434
---------- MLA ----------
López, N.I., Ruiz, J.A., Méndez, B.S. "Survival of poly-3-hydroxybutyrate-producing bacteria in soil microcosms" . World Journal of Microbiology and Biotechnology, vol. 14, no. 5, 1998, pp. 681-684.
http://dx.doi.org/10.1023/A:1008857420434
---------- VANCOUVER ----------
López, N.I., Ruiz, J.A., Méndez, B.S. Survival of poly-3-hydroxybutyrate-producing bacteria in soil microcosms. World J. Microbiol. Biotechnol. 1998;14(5):681-684.
http://dx.doi.org/10.1023/A:1008857420434