Abstract:
Various nitrogen and carbon sources were examined as inducers of the production of endoxylanase and β-xylosidase by Trametes trogii. T. trogii grown on xylan plus crystalline cellulose provided supernatants with the highest enzymatic activities. Organic nitrogen sources (especially asparagine and casamino acids) were the best for enzyme production. The increase in xylan concentration stimulated endoxylanase production whereas significant differences were not attained in β-xylosidase production with more than 5 g xylan/l in the culture medium. pH 4.0 was optimal for endoxylanase production, while β-xylosidase production was maximum at pH 5.5. Temperatures in the range of 23-28 °C stimulated enzyme production. The endoxylanase activity in the crude culture filtrate was greatest at 50 °C and pH around 5.0. The optimum pH and temperature for β-xylosidase activity were 5.5 and 50 °C respectively.
Registro:
Documento: |
Artículo
|
Título: | Influence of growth conditions on the production of xylanolytic enzymes by Trametes trogii |
Autor: | Levin, L.; Forchiassin, F. |
Filiación: | Lab. de Micología Exp., Depto. de Ciencias Biológicas, Universidad de Buenos Aires, 1428-Buenos Aires, Argentina
|
Palabras clave: | β-xylosidase; Aphyllophorales; Endoxylanase; Nutrition; asparagine; carbohydrate metabolism; carbon; casamino acid; cellulose; concentration response; endoxylanase; enzyme activity; enzyme synthesis; nitrogen metabolism; pH; xylan 1,4 beta xylosidase; xylan; Trametes trogii |
Año: | 1998
|
Volumen: | 14
|
Número: | 3
|
Página de inicio: | 443
|
Página de fin: | 446
|
DOI: |
http://dx.doi.org/10.1023/A:1008885800035 |
Título revista: | World Journal of Microbiology and Biotechnology
|
Título revista abreviado: | World J. Microbiol. Biotechnol.
|
ISSN: | 09593993
|
CODEN: | WJMBE
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v14_n3_p443_Levin |
Referencias:
- Buchert, J., Ienkanen, M., Kantelinen, A., Viikari, I., Application of xylanases in the pulp and paper industry (1994) Bioresearch Technology, 50, pp. 65-72
- Copa-Patiño, J.L., Young, G.K., Broda, P., Production and initial characterization of the xylan-degrading system of Phanerochaete chrysosporium (1993) Applied Microbiology and Biotechnology, 40, pp. 69-76
- Coughlan, M.P., Hazlewood, G.P., β-1, 4-D-Xylan-degrading enzyme systems: Biochemistry, molecular biology and applications (1993) Biotechnology and Applied Biochemistry, 17, pp. 259-289
- Eriksson, K.E., Microbial delignification - Basics, potentials and applications (1988) Biochemistry and Genetics of Cellulose Degradation, pp. 285-302. , ed Aubert, J-P., Beguin, P. & Millet, J. London: Academic Press
- Eriksson, K.E., Blanchette, R.A., Ander, P., (1990) Microbial and Enzymatic Degradation of Wood and Wood Components, , Berlin, New York: Springer-Verlag
- Hrmova, M., Biely, P., Vrsanka, M., Specificity of cellulase and β-xylanase induction in Trichoderma reesei QM 9414 (1986) Archives of Microbiology, 144, pp. 307-311
- Hrmova, M., Petrakova, E., Biely, P., Induction of cellulose and xylan-degrading enzyme systems in Aspergillus terreus by homo and heterodisaccharides composed of glucose and xylose (1991) Journal of General Microbiology, 137, pp. 541-547
- Khandke, K.M., Vithayathil, P.J., Murthy, S.K., Purification of xylanase, β-glucosidase, enclocellulase and exocellulase from a thermophilic fungus Thermoascus aurantiacus (1989) Archives of Biochemistry and Biophysics, 274, pp. 491-500
- Levin, L., Forchiassin, F., Influencia de las fuentes carbonadas y nitrogenadas sobre la actividad celulolítica de Trametes trogii (1995) Revista Argentina de Microbiología, 27, pp. 11-20
- Maijala, P., Raudaskoski, M., Viikari, L., Hemicellulolytic enzymes in P- and S-strains of Heterobasidion annosum (1995) Microbiology, 141, pp. 743-750
- Peltonen, S., Kayalainen, R., Niku-Paavola, M.L., Purification and characterization of a xylanase from Bipolaris sorokiniana (1994) Mycological Research, 98, pp. 67-73
- Planes, E., Bassi, M.H., Bensignor, J., Burachik, M., Pulpado biológico de maderas de sauce (1985) 21 Congreso Técnico Sobre Celulosa y Papel, , Buenos Aires, Argentina
- Royer, C., Nakas, J.P., Interrelationship of xylanase induction and cellulase induction of Trichoderma longibrachiatum (1990) Applied and Environmental Microbiology, 56, pp. 2535-2539
- Senior, D.J., Mayers, P.R., Saddler, J.N., Xylanase production by Trichoderma harzianum ES8 (1989) Applied Microbiology and Biotechnology, 32, pp. 137-142
- Silva, H., Landa, A., Agosin, E., Aislamiento, selección y caracterización de hongos ligninolíticos chilenos (1990) Archivos de Biología y Medicina Experimentales, 23, pp. 41-49
- Wong, K.K.Y., Multiplicity of β-1,4-xylanase in microorganisms: Functions and applications (1988) Microbiological Reviews, 52, pp. 305-317
- Wright, J.E., Deschamps, J.R., Rovetta, G., Basidiomycetes xilófilos de la región mesopotámica. I. Poliporos trametoides (1973) Revista de Investigaciones Agropecuarias del INTA Serie V, 10, pp. 117-174
Citas:
---------- APA ----------
Levin, L. & Forchiassin, F.
(1998)
. Influence of growth conditions on the production of xylanolytic enzymes by Trametes trogii. World Journal of Microbiology and Biotechnology, 14(3), 443-446.
http://dx.doi.org/10.1023/A:1008885800035---------- CHICAGO ----------
Levin, L., Forchiassin, F.
"Influence of growth conditions on the production of xylanolytic enzymes by Trametes trogii"
. World Journal of Microbiology and Biotechnology 14, no. 3
(1998) : 443-446.
http://dx.doi.org/10.1023/A:1008885800035---------- MLA ----------
Levin, L., Forchiassin, F.
"Influence of growth conditions on the production of xylanolytic enzymes by Trametes trogii"
. World Journal of Microbiology and Biotechnology, vol. 14, no. 3, 1998, pp. 443-446.
http://dx.doi.org/10.1023/A:1008885800035---------- VANCOUVER ----------
Levin, L., Forchiassin, F. Influence of growth conditions on the production of xylanolytic enzymes by Trametes trogii. World J. Microbiol. Biotechnol. 1998;14(3):443-446.
http://dx.doi.org/10.1023/A:1008885800035