Navegar

Colección

Datos Estadísticas

Artículo

Ghersa, F.; Burdisso, J.; Vallcaneras, S.S.; Fuentes, F.; de la Vega, M.; Delgado, S.M.; Telleria, C.M.; Casais, M. "Neuromodulation of the luteal regression: Presence of progesterone receptors in coeliac ganglion" (2015) Experimental Physiology. 100(8):935-946
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

New Findings: What is the central question of this study? The processes involved in luteal involution have not yet been clarified and, in general, have been studied only from a hormonal point of view. We investigated whether progesterone, from the coeliac ganglion through the superior ovarian nerve, is able to modify the luteal regression of late pregnancy in the rat. What is the main finding and its importance? We showed that the luteal regression might be reversed by the neural effect of progesterone and demonstrated the presence of its receptors in the coeliac ganglion. This suggests that the peripheral neural pathway, through neuron-hormone interaction, represents an additional mechanism to control luteal function in addition to the classical endocrine regulation. The corpus luteum (CL) is a transitory endocrine gland that produces progesterone (P). At the end of its useful life, it suffers a process of functional and structural regression until its complete disappearance from the ovary. To investigate whether P is able to regulate the process of luteal regression through the peripheral neural pathway, we used the coeliac ganglion (CG)-superior ovarian nerve-ovary system from rats on day 21 of pregnancy. We stimulated the CG with P and analysed the functional regression through ovarian P release measured by radioimmunoassay, expression by RT-PCR and activity of luteal 3β- and 20α-hydroxysteroid dehydrogenase (anabolic and catabolic P enzymes, respectively). The luteal structural regression was evaluated through a study of apoptosis measured by TUNEL assay and the expression of apoptotic factors, such as Bcl-2, Bax, Fas and Fas ligand (FasL) by RT-PCR. To explore whether the effects mediated by P on the CL may be associated with P receptors, their presence in the CG was investigated by immunohistochemistry. In the group stimulated with P in the CG, the ovarian P release and the 3β-hydroxysteroid dehydrogenase activity increased, whereas the expression and activity of 20α-hydroxysteroid dehydrogenase decreased. In addition, a decrease in the number of apoptotic nuclei and a decrease of the expression of FasL were observed. We demonstrated the presence of P receptors in the CG. Overall, our results suggest that the regression of the CL of late pregnancy may be reprogrammed through the peripheral neural pathway, and this effect might be mediated by P bound to its receptor in the CG. © 2015 The Physiological Society.

Registro:

Documento: Artículo
Título:Neuromodulation of the luteal regression: Presence of progesterone receptors in coeliac ganglion
Autor:Ghersa, F.; Burdisso, J.; Vallcaneras, S.S.; Fuentes, F.; de la Vega, M.; Delgado, S.M.; Telleria, C.M.; Casais, M.
Filiación:Laboratorio de Biología de la Reproducción (LABIR), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina
Instituto Multidisciplinario de Investigaciones Biológicas de San Luis (IMIBIO-SL), CONICET, San Luis, Argentina
Centro de Microscopías Avanzadas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
Dpto. de Química Orgánica, CIHIDECAR-CONICET, FCEyN, UBA. Int., Guiraldes 2160, Pabellón II, Cdad. Universitaria, Ciudad Autónoma de Buenos Aires, 1428, Argentina
Division of Basic Biomedical Sciences, Sanford School of Medicine of the University of South Dakota, Vermillion, United States
Palabras clave:20alpha hydroxysteroid dehydrogenase; 3(or 17)beta hydroxysteroid dehydrogenase; beta 3 tubulin; beta tubulin; Fas antigen; Fas ligand; messenger RNA; progesterone; progesterone receptor; protein Bax; protein bcl 2; unclassified drug; agents interacting with transmitter, hormone or drug receptors; progesterone; progesterone receptor; animal experiment; apoptosis; Article; celiac ganglion; cell nucleus; cellular distribution; controlled study; corpus luteum; cytoplasm; down regulation; enzyme activity; ex vivo study; female; immunohistochemistry; immunoreactivity; luteal cell; luteal phase; nerve; neuromodulation; nonhuman; peripheral nerve; peripheral nervous system; progesterone release; protein expression; radioimmunoassay; rat; reverse transcription polymerase chain reaction; superior ovarian nerve; third trimester pregnancy; TUNEL assay; Western blotting; agonists; animal; corpus luteum; drug effects; luteolysis; organ culture technique; ovary; physiology; pregnancy; Sprague Dawley rat; sympathetic ganglion; Animals; Corpus Luteum; Female; Ganglia, Sympathetic; Luteolysis; Neurotransmitter Agents; Organ Culture Techniques; Ovary; Pregnancy; Progesterone; Rats; Rats, Sprague-Dawley; Receptors, Progesterone
Año:2015
Volumen:100
Número:8
Página de inicio:935
Página de fin:946
DOI: http://dx.doi.org/10.1113/EP085261
Título revista:Experimental Physiology
Título revista abreviado:Exp. Physiol.
ISSN:09580670
CODEN:EXPHE
CAS:20alpha hydroxysteroid dehydrogenase, 9040-08-8; 3(or 17)beta hydroxysteroid dehydrogenase, 9015-81-0; beta tubulin, 87090-36-6; progesterone, 57-83-0; protein bcl 2, 219306-68-0; Neurotransmitter Agents; Progesterone; Receptors, Progesterone
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09580670_v100_n8_p935_Ghersa

Referencias:

  • Blaustein, J.D., Lehman, M.N., Turcotte, J.C., Greene, G., Estrogen receptors in dendrites and axon terminals in the guinea pig hypothalamus (1992) Endocrinology, 131, pp. 281-290
  • Bowen-Shauver, J.M., Tessier, C., Prigent-Tessier, A., Bao, L., Telleria, C.M., Ferguson-Gottschall, S., Gibori, G.B., Gibori, G., Decidual activin: its role in the apoptotic process and its regulation by prolactin (2003) Biol Reprod, 68, pp. 1687-1694
  • Bramley, T.A., Menzies, G.S., Particulate binding sites for steroid hormones in subcellular fractions of the ovine corpus luteum: properties and hormone specificity (1994) Mol Cell Endocrinol, 103, pp. 39-48
  • Casais, M., Sosa, Z.Y., Rastrilla, A.M., Aguado, L.I., Coeliac ganglion adrenergic activity modifies ovarian progesterone during pregnancy: its inter-relationship with LH (2001) J Endocrinol, 170, pp. 575-584
  • Chan, J.R., Rodriguez-Waitkus, P.M., Ng, B.K., Liang, P., Glaser, M., Progesterone synthesized by Schwann cells during myelin formation regulates neuronal gene expression (2000) Mol Biol Cell, 11, pp. 2283-2295
  • Chau, Y.P., Chien, C.L., Lu, K.S., The permeability of capillaries among the small granule-containing cells in rat superior cervical ganglia: an ultrastructural lanthanum tracer study (1991) Histol Histopathol, 6, pp. 261-268
  • Chau, Y.P., Lu, K.S., Investigation of the blood-ganglion barrier properties in rat sympathetic ganglia by using lanthanum ion and horseradish peroxidase as tracers (1995) Acta Anat (Basel), 153, pp. 135-144
  • DonCarlos, L.L., Garcia-Ovejero, D., Sarkey, S., Garcia-Segura, L.M., Azcoitia, I., Androgen receptor immunoreactivity in forebrain axons and dendrites in the rat (2003) Endocrinology, 144, pp. 3632-3638
  • DonCarlos, L.L., Sarkey, S., Lorenz, B., Azcoitia, I., Garcia-Ovejero, D., Huppenbauer, C., Garcia-Segura, L.M., Novel cellular phenotypes and subcellular sites for androgen action in the forebrain (2006) Neuroscience, 138, pp. 801-807
  • Eränkö, O., Small intensely fluorescent (SIF) cells and nervous transmission in sympathetic ganglia (1978) Annu Rev Pharmacol Toxicol, 18, pp. 417-430
  • Hashimoto, N., Yamanaka, H., Mizushima, T., Noguchi, K., Increased expression of 3β-hydroxysteroid dehydrogenase mRNA in dorsal root ganglion neurons of adult rats following peripheral nerve injury (2003) Neurosci Lett, 340, pp. 45-48
  • Klein, C.M., Burden, H.W., Anatomical localization of afferent and postganglionic sympathetic neurons innervating the rat ovary (1988) Neurosci Lett, 85, pp. 217-222
  • Koenig, H.L., Schumacher, M., Ferzaz, B., Thi, A.N., Ressouches, A., Guennoun, R., Jung-Testas, I., Baulieu, E.E., Progesterone synthesis and myelin formation by Schwann cells (1995) Science, 268, pp. 1500-1503
  • Kuranaga, E., Kanuka, H., Furuhata, Y., Yonezawa, T., Suzuki, M., Nishihara, M., Takahashi, M., Requirement of the Fas ligand-expressing luteal immune cells for regression of corpus luteum (2000) FEBS Lett, 472, pp. 137-142
  • Kuranaga, E., Kanuka, H., Hirabayashi, K., Suzuki, M., Nishihara, M., Takahashi, M., Progesterone is a cell death suppressor that downregulates Fas expression in rat corpus luteum (2000) FEBS Lett, 466, pp. 279-282
  • Labombarda, F., Gonzalez, S.L., Deniselle, M.C., Vinson, G.P., Schumacher, M., De Nicola, A.F., Guennoun, R., Effects of injury and progesterone treatment on progesterone receptor and progesterone binding protein 25-Dx expression in the rat spinal cord (2003) J Neurochem, 87, pp. 902-913
  • Nagata, S., Golstein, P., The Fas death factor (1995) Science, 267, pp. 1449-1456
  • Oltvai, Z.N., Milliman, C.L., Korsmeyer, S.J., Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death (1993) Cell, 74, pp. 609-619
  • Prud'homme, M.J., Houdeau, E., Serghini, R., Tillet, Y., Schemann, M., Rousseau, J.P., Small intensely fluorescent cells of the rat paracervical ganglion synthesize adrenaline, receive afferent innervation from postganglionic cholinergic neurones, and contain muscarinic receptors (1999) Brain Res, 821, pp. 141-149
  • Rothchild, I., The regulation of the mammalian corpus luteum (1981) Recent Prog Horm Res, 37, pp. 183-298
  • Roughton, S.A., Lareu, R.R., Bittles, A.H., Dharmarajan, A.M., Fas and Fas ligand messenger ribonucleic acid and protein expression in the rat corpus luteum during apoptosis-mediated luteolysis (1999) Biol Reprod, 60, pp. 797-804
  • Schumacher, M., Mattern, C., Ghoumari, A., Oudinet, J.P., Liere, P., Labombarda, F., Sitruk-Ware, R., Guennoun, R., Revisiting the roles of progesterone and allopregnanolone in the nervous system: resurgence of the progesterone receptors (2014) Prog Neurobiol, 113, pp. 6-39
  • Stocco, C., Kwintkiewicz, J., Cai, Z., Identification of regulatory elements in the Cyp19 proximal promoter in rat luteal cells (2007) J Mol Endocrinol, 39, pp. 211-221
  • Stocco, C.O., Deis, R.P., Participation of intraluteal progesterone and prostaglandin F2α in LH-induced luteolysis in pregnant rat (1998) J Endocrinol, 156, pp. 253-259
  • Sugino, N., Okuda, K., Species-related differences in the mechanism of apoptosis during structural luteolysis (2007) J Reprod Dev, 53, pp. 977-986
  • Sugino, N., Telleria, C.M., Gibori, G., Progesterone inhibits 20α-hydroxysteroid dehydrogenase expression in the rat corpus luteum through the glucocorticoid receptor (1997) Endocrinology, 138, pp. 4497-4500
  • Telleria, C.M., Deis, R.P., Effect of RU486 on ovarian progesterone production at pro-oestrus and during pregnancy: a possible dual regulation of the biosynthesis of progesterone (1994) J Reprod Fertil, 102, pp. 379-384
  • Telleria, C.M., Stocco, C.O., Stati, A.O., Deis, R.P., Progesterone receptor is not required for progesterone action in the rat corpus luteum of pregnancy (1999) Steroids, 64, pp. 760-766
  • Vallcaneras, S.S., Casais, M., Anzulovich, A.C., Delgado, S.M., Sosa, Z., Telleria, C.M., Rastrilla, A.M., Androstenedione acts on the coeliac ganglion and modulates luteal function via the superior ovarian nerve in the postpartum rat (2011) J Steroid Biochem Mol Biol, 125, pp. 243-250
  • Vallcaneras, S.S., Casais, M., Delgado, S.M., Filippa, V., Mohamed, F., Sosa, Z., Rastrilla, A.M., Androgen receptors in coeliac ganglion in late pregnant rat (2009) Steroids, 74, pp. 526-534
  • Vega Orozco, A., Daneri, C., Anesetti, G., Cabrera, R., Sosa, Z., Rastrilla, A.M., Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat (2012) Reproduction, 143, pp. 183-193
  • Waters, E.M., Torres-Reveron, A., McEwen, B.S., Milner, T.A., Ultrastructural localization of extranuclear progestin receptors in the rat hippocampal formation (2008) J Comp Neurol, 511, pp. 34-46
  • Williams, G.T., Smith, C.A., Molecular regulation of apoptosis: genetic controls on cell death (1993) Cell, 74, pp. 777-779

Citas:

---------- APA ----------
Ghersa, F., Burdisso, J., Vallcaneras, S.S., Fuentes, F., de la Vega, M., Delgado, S.M., Telleria, C.M.,..., Casais, M. (2015) . Neuromodulation of the luteal regression: Presence of progesterone receptors in coeliac ganglion. Experimental Physiology, 100(8), 935-946.
http://dx.doi.org/10.1113/EP085261
---------- CHICAGO ----------
Ghersa, F., Burdisso, J., Vallcaneras, S.S., Fuentes, F., de la Vega, M., Delgado, S.M., et al. "Neuromodulation of the luteal regression: Presence of progesterone receptors in coeliac ganglion" . Experimental Physiology 100, no. 8 (2015) : 935-946.
http://dx.doi.org/10.1113/EP085261
---------- MLA ----------
Ghersa, F., Burdisso, J., Vallcaneras, S.S., Fuentes, F., de la Vega, M., Delgado, S.M., et al. "Neuromodulation of the luteal regression: Presence of progesterone receptors in coeliac ganglion" . Experimental Physiology, vol. 100, no. 8, 2015, pp. 935-946.
http://dx.doi.org/10.1113/EP085261
---------- VANCOUVER ----------
Ghersa, F., Burdisso, J., Vallcaneras, S.S., Fuentes, F., de la Vega, M., Delgado, S.M., et al. Neuromodulation of the luteal regression: Presence of progesterone receptors in coeliac ganglion. Exp. Physiol. 2015;100(8):935-946.
http://dx.doi.org/10.1113/EP085261