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Abstract:

An automated micellar electrokinetic capillary chromatographic method has been developed in order to determine xanthines, e.g. caffeine, theobromine and theophylline, and chlorogenic acid in samples of yerba mate (Ilex paraguariensis). The target constituents were detected by photodiode array, and quantified by an external standard method. In addition, each constituent was collected separately and identified by EIMS. The method has been used to analyse 30 samples of mate infusions prepared at 30 and 75°C with milled leaves and stems of 14 commercial brands which had been subjected to different elaboration process. Suspended powdered material of each infusion was also analysed after three sieving steps. There was a remarkable difference in the relative xanthine composition of the finely suspended material, the amount of which varied according to the yerba mate brand, the elaboration process and the temperature of the infusion. The importance of these results with respect to gastrointestinal disorders which have been observed by habitual consumers of mate are discussed. Copyright © 2002 John Wiley & Sons, Ltd.

Registro:

Documento: Artículo
Título:High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography
Autor:Pomilio, A.B.; Trajtemberg, S.; Vitale, A.A.
Filiación:PROPLAME-CONICET, Departamento de Química Orgánica, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Palabras clave:Electron-impact mass spectrometry; High-performance capillary electrophoresis; Ilex paraguariensis; Mate infusions; Micellar electrokinetic capillary chromatography; Xanthines; caffeine; chlorogenic acid; theobromine; theophylline; xanthine derivative; analytic method; article; capillary electrophoresis; consumer; controlled study; gastrointestinal disease; ilex; micellar electrokinetic chromatography; nonhuman; phytochemistry; plant; plant leaf; plant stem; powder; quantitative analysis; temperature; automation; chemistry; electrospray mass spectrometry; Ilex paraguariensis; methodology; plant stem; Automation; Chromatography, Micellar Electrokinetic Capillary; Ilex paraguariensis; Plant Leaves; Plant Stems; Spectrometry, Mass, Electrospray Ionization; Support, Non-U.S. Gov't; Automation; Plant Leaves; Spectrometry, Mass, Electrospray Ionization; Croton ovalifolius; Ilex; Ilex paraguariensis
Año:2002
Volumen:13
Número:4
Página de inicio:235
Página de fin:241
DOI: http://dx.doi.org/10.1002/pca.647
Título revista:Phytochemical Analysis
Título revista abreviado:Phytochem. Anal.
ISSN:09580344
CODEN:PHANE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09580344_v13_n4_p235_Pomilio

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Citas:

---------- APA ----------
Pomilio, A.B., Trajtemberg, S. & Vitale, A.A. (2002) . High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography. Phytochemical Analysis, 13(4), 235-241.
http://dx.doi.org/10.1002/pca.647
---------- CHICAGO ----------
Pomilio, A.B., Trajtemberg, S., Vitale, A.A. "High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography" . Phytochemical Analysis 13, no. 4 (2002) : 235-241.
http://dx.doi.org/10.1002/pca.647
---------- MLA ----------
Pomilio, A.B., Trajtemberg, S., Vitale, A.A. "High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography" . Phytochemical Analysis, vol. 13, no. 4, 2002, pp. 235-241.
http://dx.doi.org/10.1002/pca.647
---------- VANCOUVER ----------
Pomilio, A.B., Trajtemberg, S., Vitale, A.A. High-performance capillary electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography. Phytochem. Anal. 2002;13(4):235-241.
http://dx.doi.org/10.1002/pca.647