Artículo

Lauricella, A.M.; Quintana, I.; Castañon, M.; Sassetti, B.; Kordich, L. "Influence of homocysteine on fibrin network lysis" (2006) Blood Coagulation and Fibrinolysis. 17(3):181-186
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Abstract:

To elucidate some of the links between homocysteine and vascular disease, we have evaluated the effect of the amino acid on the formation (by kinetics studies), structure (by electron microscopy) and lysis of the fibrin network, using tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA). We have studied whether homocysteine could alter the activity of the components involved in fibrinolysis (by amidolytic and thrombolytic methods). The results showed that homocysteine-associated networks were more compact and branched than controls (52 ± 6 vs 44 ± 5 fibers/field, P = 0.008), and were formed by shorter and thicker fibers. This clot proved to be more resistant to fibrinolysis with u-PA than control [lysis time 50%: 257 ± 16 (homocysteine) vs 187 ± 6 min (control); P < 0.004], but there were no differences with t-PA. Homocysteine did not affect the biological activities of plasmin, or plasminogen activation by t-PA and u-PA. Defective fibrinolysis with u-PA was therefore associated with homocysteine-fibrin structural alterations rather than the homocysteine effect on the biological activities of the fibrinolytic components evaluated. Results suggest that hyperhomocysteinemic patients could produce tight clots, were more resistant to lysis, and generated a procoagulant environment in situ. We believe that our findings may contribute to understanding the mechanisms involved in the homocysteine harmful effect. © 2006 Lippincott Williams & Wilkins.

Registro:

Documento: Artículo
Título:Influence of homocysteine on fibrin network lysis
Autor:Lauricella, A.M.; Quintana, I.; Castañon, M.; Sassetti, B.; Kordich, L.
Filiación:Department of Biological Chemistry, School of Exact and Natural Sciences, Buenos Aires University, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (C1428EHA) Buenos Aires, Argentina
Palabras clave:Fibrin networks; Fibrinolysis; Homocysteine; fibrin; homocysteine; plasminogen; tissue plasminogen activator; urokinase; amino acid analysis; article; binding kinetics; controlled study; electron microscopy; fiber; fibrin metabolism; genetic resistance; human; human cell; hyperhomocysteinemia; lysis; plasminogen activation; priority journal; regulatory mechanism; thickness; vascular disease; Blood Coagulation; Fibrin; Fibrinolysis; Homocysteine; Humans; Microscopy, Electron; Plasma; Plasmin; Plasminogen Activator Inhibitor 1; Plasminogen Activators; Plasminogen Inactivators
Año:2006
Volumen:17
Número:3
Página de inicio:181
Página de fin:186
DOI: http://dx.doi.org/10.1097/01.mbc.0000220238.99843.45
Título revista:Blood Coagulation and Fibrinolysis
Título revista abreviado:Blood Coagul. Fibrinolysis
ISSN:09575235
CODEN:BLFIE
CAS:fibrin, 9001-31-4; homocysteine, 454-28-4, 6027-13-0; plasminogen, 9001-91-6; tissue plasminogen activator, 105913-11-9; urokinase, 139639-24-0; Fibrin, 9001-31-4; Homocysteine, 454-28-4; Plasmin, EC 3.4.21.7; Plasminogen Activator Inhibitor 1; Plasminogen Activators, EC 3.4.21.-; Plasminogen Inactivators
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09575235_v17_n3_p181_Lauricella

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Citas:

---------- APA ----------
Lauricella, A.M., Quintana, I., Castañon, M., Sassetti, B. & Kordich, L. (2006) . Influence of homocysteine on fibrin network lysis. Blood Coagulation and Fibrinolysis, 17(3), 181-186.
http://dx.doi.org/10.1097/01.mbc.0000220238.99843.45
---------- CHICAGO ----------
Lauricella, A.M., Quintana, I., Castañon, M., Sassetti, B., Kordich, L. "Influence of homocysteine on fibrin network lysis" . Blood Coagulation and Fibrinolysis 17, no. 3 (2006) : 181-186.
http://dx.doi.org/10.1097/01.mbc.0000220238.99843.45
---------- MLA ----------
Lauricella, A.M., Quintana, I., Castañon, M., Sassetti, B., Kordich, L. "Influence of homocysteine on fibrin network lysis" . Blood Coagulation and Fibrinolysis, vol. 17, no. 3, 2006, pp. 181-186.
http://dx.doi.org/10.1097/01.mbc.0000220238.99843.45
---------- VANCOUVER ----------
Lauricella, A.M., Quintana, I., Castañon, M., Sassetti, B., Kordich, L. Influence of homocysteine on fibrin network lysis. Blood Coagul. Fibrinolysis. 2006;17(3):181-186.
http://dx.doi.org/10.1097/01.mbc.0000220238.99843.45