Artículo
Quintana, I.; Freeman, D.; Galarza, C.; Murua, A.; Spence, J.D.; Kordich, L. "Validation of an enzyme immunoassay for the determination of total homocysteine in plasma" (2000) Blood Coagulation and Fibrinolysis. 11(3):235-238
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Abstract:
In recent years, the determination of homocysteine (Hcy) has become increasingly important, since high levels of Hcy in plasma or serum represent an independent risk factor for occlusive vascular diseases. Nowadays, clinical laboratories use several analytical techniques to measure Hcy, of which high-performance liquid chromatography (HPLC) is the most popular. Recently, assays for Hcy quantification based on enzyme immunoassays (EIA) have become commercially available. Our group carried out the validation of the Axis method and compared results with those obtained by an established HPLC assay. Intra- and inter-assay coefficients of variation were ≤ 8.5%. Compared with HPLC, linear regression analysis showed r = 0.984, slope = 0.952, intercept = 1.24 μmol/l; Bland-Altman procedure, the mean of the difference EIA-HPLC results = 0.5 μmol/l. Our results suggest that Hcy determinations by both methods are equivalent, and that the Axis assay provides reproducible and reliable data. (C) 2000 Lippincott Williams and Wilkins.
Registro:
| Documento: | Artículo |
| Título: | Validation of an enzyme immunoassay for the determination of total homocysteine in plasma |
| Autor: | Quintana, I.; Freeman, D.; Galarza, C.; Murua, A.; Spence, J.D.; Kordich, L. |
| Filiación: | Lab. de Hemostasia y Trombosis, Depto. de Quimica Biológica, Facultad de Cie. Exact. y Naturales, Pabellon II, Cd. Universitaria, 1428 Buenos Aires, Argentina |
| Palabras clave: | Enzyme immunoassay; Homocysteine; Validation; homocysteine; article; enzyme immunoassay; high performance liquid chromatography; human; human experiment; intermethod comparison; normal human; priority journal; Homocysteine; Humans; Immunoenzyme Techniques; Sensitivity and Specificity |
| Año: | 2000 |
| Volumen: | 11 |
| Número: | 3 |
| Página de inicio: | 235 |
| Página de fin: | 238 |
| Título revista: | Blood Coagulation and Fibrinolysis |
| Título revista abreviado: | Blood Coagul. Fibrinolysis |
| ISSN: | 09575235 |
| CODEN: | BLFIE |
| CAS: | Homocysteine, 454-28-4 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09575235_v11_n3_p235_Quintana |
Referencias:
- Finkelstein, J.D., Methionine metabolism in mammals (1990) J Nutr Biochem, 1, pp. 228-237
- Ueland, M., Homocysteine species as components of plasma redox thiol status (1995) Clin Chem, 41, pp. 340-342
- Malinow, M.R., Hyperhomocyst(e)inemia. A common and easily reversible risk factor for occlusive atherosclerosis (1990) Circulation, 81, pp. 2004-2006
- D'Angelo, A., Selhub, J., Homocysteine and thrombotic disease (1997) Blood, 90, pp. 1-11
- Grahan, I.M., Daly, L.E., Refsum, H.M., Robinson, K., Brattsrom, L.E., Ueland, P.M., Plama homocysteine as a risk factor for vascular disease: The European Concerted Action Project (1997) JAMA, 277, pp. 1775-1781
- Ueland, P.M., Refsum, H., Stabler, S., Malinow, M.R., Andersson, A., Allen, R.H., Total homocysteine in plasma or serum: Methods and clinical applications (1993) Clin Chem, 39, pp. 1764-1779
- Araki, A., Sako, Y., Determination of free and total homocysteine in human plasma by high-performance liquid chromatography with fluorescence detection (1987) J Chromatogr, 422, pp. 43-52
- Ubbink, J.B., Vermaak, W.J.H., Bissbort, S., Rapid high-performance liquid chromatographic assay for total homocysteine levels in human serum (1991) J Chromatogr, 565, pp. 441-446
- Jacobsen, D.W., Gatautis, V.J., Green, R., Robinson, K., Savon, S., Secic, M., Rapid HPLC determination of total homocysteine and other thiols in serum and plasma: Sex differences and correlation with cobalamin and folate concentrations in healthy subjects (1994) Clin Chem, 40, pp. 873-881
- Bland, J.M., Altman, D.G., Statistical methods for assessing agreement between two methods of clinical measurement (1986) Lancet, 8, pp. 307-310
- Frantzen, F., Faaren, A.L., Alfheim, I., Nordhei, A.K., Enzyme conversion immunoassay for determining total homocysteine in plasma or serum (1998) Clin Chem, 44, pp. 311-316
- Pietzsch, J., Pixa, A., Determination of total homocysteine (1998) Clin Chem, 44, pp. 1781-1782
Citas:
---------- APA ----------
Quintana, I., Freeman, D., Galarza, C., Murua, A., Spence, J.D. & Kordich, L. (2000) . Validation of an enzyme immunoassay for the determination of total homocysteine in plasma. Blood Coagulation and Fibrinolysis, 11(3), 235-238.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09575235_v11_n3_p235_Quintana [ ]
---------- CHICAGO ----------
Quintana, I., Freeman, D., Galarza, C., Murua, A., Spence, J.D., Kordich, L. "Validation of an enzyme immunoassay for the determination of total homocysteine in plasma" . Blood Coagulation and Fibrinolysis 11, no. 3 (2000) : 235-238.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09575235_v11_n3_p235_Quintana [ ]
---------- MLA ----------
Quintana, I., Freeman, D., Galarza, C., Murua, A., Spence, J.D., Kordich, L. "Validation of an enzyme immunoassay for the determination of total homocysteine in plasma" . Blood Coagulation and Fibrinolysis, vol. 11, no. 3, 2000, pp. 235-238.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09575235_v11_n3_p235_Quintana [ ]
---------- VANCOUVER ----------
Quintana, I., Freeman, D., Galarza, C., Murua, A., Spence, J.D., Kordich, L. Validation of an enzyme immunoassay for the determination of total homocysteine in plasma. Blood Coagul. Fibrinolysis. 2000;11(3):235-238.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09575235_v11_n3_p235_Quintana [ ]
