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Abstract:

Cellular microarrays present a promising tool for multiplex evaluation of the signalling effect of substrate-immobilized factors on cellular differentiation. In this paper, we compare the early myoblast-to-osteoblast cell commitment steps in response to a growth factor stimulus using standard well plate differentiation assays or cellular microarrays. Our results show that restraints on the cell culture size, inherent to cellular microarrays, impair the differentiation outcome. Also, while cells growing on spots with immobilised BMP-2 are early biased towards the osteoblast fate, longer periods of cell culturing in the microarrays result in cell proliferation and blockage of osteoblast differentiation. The results presented here raise concerns about the efficiency of cell differentiation when the cell culture dimensions are reduced to a simplified microspot environment. Also, these results suggest that further efforts should be devoted to increasing the complexity of the microspots composition, aiming to replace signalling cues missing in this system. © 2012 Springer Science+Business Media New York.

Registro:

Documento: Artículo
Título:Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays
Autor:Rodríguez-Seguí, S.A.; Ortuño, M.J.; Ventura, F.; Martínez, E.; Samitier, J.
Filiación:Nanobioengineering Group, Institute for Bioengineering of Catalonia, Baldiri i Reixac 10-12, 08028 Barcelona, Spain
Department of Electronics, University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, 08028, Barcelona, Spain
Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Ciudad Universitaria, Intendente Guiraldes 2160, Pab. II, 2do piso, C1428EGA Buenos Aires, Argentina
Departament de Ciències Fisiològiques II, IDIBELL, L'Hospitalet de Llobregat, Barcelona, Spain
Palabras clave:Cell differentiation; Cellular differentiation; Cellular microarrays; Growth factor; Microenvironments; Osteoblast differentiation; Well plates; Cell proliferation; Cell culture; bone morphogenetic protein 2; fibronectin; laminin; transcription factor osterix; article; cell adhesion; cell culture; cell differentiation; cell fate; cell proliferation; cell size; controlled study; human; human cell; immobilization; immunohistochemistry; microarray analysis; microenvironment; microtechnology; molecular imaging; myoblast; osteoblast; priority journal; protein analysis; protein expression; signal transduction; Animals; Bone Morphogenetic Protein 2; Cell Differentiation; Cellular Microenvironment; CHO Cells; Cricetinae; Cricetulus; Humans; Mice; Protein Array Analysis
Año:2013
Volumen:24
Número:1
Página de inicio:189
Página de fin:198
DOI: http://dx.doi.org/10.1007/s10856-012-4785-1
Título revista:Journal of Materials Science: Materials in Medicine
Título revista abreviado:J. Mater. Sci. Mater. Med.
ISSN:09574530
CODEN:JSMME
CAS:fibronectin, 86088-83-7; laminin, 2408-79-9; Bone Morphogenetic Protein 2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09574530_v24_n1_p189_RodriguezSegui

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Citas:

---------- APA ----------
Rodríguez-Seguí, S.A., Ortuño, M.J., Ventura, F., Martínez, E. & Samitier, J. (2013) . Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays. Journal of Materials Science: Materials in Medicine, 24(1), 189-198.
http://dx.doi.org/10.1007/s10856-012-4785-1
---------- CHICAGO ----------
Rodríguez-Seguí, S.A., Ortuño, M.J., Ventura, F., Martínez, E., Samitier, J. "Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays" . Journal of Materials Science: Materials in Medicine 24, no. 1 (2013) : 189-198.
http://dx.doi.org/10.1007/s10856-012-4785-1
---------- MLA ----------
Rodríguez-Seguí, S.A., Ortuño, M.J., Ventura, F., Martínez, E., Samitier, J. "Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays" . Journal of Materials Science: Materials in Medicine, vol. 24, no. 1, 2013, pp. 189-198.
http://dx.doi.org/10.1007/s10856-012-4785-1
---------- VANCOUVER ----------
Rodríguez-Seguí, S.A., Ortuño, M.J., Ventura, F., Martínez, E., Samitier, J. Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays. J. Mater. Sci. Mater. Med. 2013;24(1):189-198.
http://dx.doi.org/10.1007/s10856-012-4785-1