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Abstract:

Endotoxins, also referred to as pyrogens, are lipopolysaccharides (LPS) present in the outer membrane of Gram-negative bacteria, and represent one of the most dangerous microbiological contaminants in water for hemodialysis and intravenous infusion. A method is presented for the simultaneous detection of endotoxins and other bacterial lysis contaminating species in purified water for parenteral formulations. The technique used is electrochemical impedance spectroscopy, with data interpretation using principal component analysis (PCA), cluster analysis (CA), and multivariate discriminant analysis (MDA). Two types of electrode surfaces were modified with LPS recognition agents: (i) a 37 amino acids fragment of a 18. kDa cationic antimicrobial protein (CAP18F) that has LPS binding activity; (ii) the highly selective endotoxin neutralizing protein (ENP). Statistical multivariate analysis of the impedance spectral data allowed the detection of endotoxin at, and below, the threshold pharmaceutical regulatory level. Discrimination of LPS from samples containing proteins, nucleic acids, phospholipids or their mixtures was achieved. These results open a new route to a practical instrumental method capable of detecting and discriminating LPS from other potential pro-inflammatory species of microbiological origin, such as nucleic acids. © 2010 Elsevier B.V.

Registro:

Documento: Artículo
Título:Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin
Autor:Heras, J.Y.; Pallarola, D.; Battaglini, F.
Filiación:INQUIMAE - Departamento de Química Inorgánica, Analítica y Química Física, Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón 2, C1428EHA Buenos Aires, Argentina
Instituto de Investigaciones Fiscoquímicas Teóricas y Aplicadas (INIFTA), CONICET, Universidad Nacional de La Plata, CC. 16 Suc. 4, La Plata 1900, Argentina
Palabras clave:Electronic tongue; Endotoxin detection; Impedance spectroscopy; Principal component analysis; Pro-inflammatory molecules; Antimicrobial proteins; Bacterial lysis; Binding activities; Data interpretation; Electrode surfaces; Electronic tongue; Endotoxin detection; Endotoxin neutralizing; Gram-negative bacteria; Impedance spectroscopy; Inflammatory species; Instrumental methods; Lipopolysaccharides; Multi variate analysis; Multivariate discriminant analysis; Outer membrane; Purified water; Regulatory level; Simultaneous detection; Spectral data; Amino acids; Bacteria; Bacteriology; Cluster analysis; Decision making; Dialysis; Discriminant analysis; Drug products; Electrochemical corrosion; Electrochemical impedance spectroscopy; Electrodes; Electronic tongues; Multivariant analysis; Nucleic acids; Organic acids; Phospholipids; Proteins; Spectrum analysis; Principal component analysis; amino acid; cationic antimicrobial protein; endotoxin; endotoxin neutralizing protein; lipopolysaccharide; nucleic acid; phospholipid; protein; unclassified drug; biomimetic material; drug mixture; endotoxin; lipopolysaccharide; article; bacterium contamination; cluster analysis; controlled study; discriminant analysis; electrochemical impedance spectroscopy; electrode; electronics; nonhuman; principal component analysis; protein binding; tongue; toxin analysis; analysis; conductometry; device failure analysis; devices; drug mixture; equipment design; evaluation study; genetic procedures; metabolism; reproducibility; Salmonella enterica; sensitivity and specificity; Biomimetic Materials; Biosensing Techniques; Complex Mixtures; Conductometry; Endotoxins; Equipment Design; Equipment Failure Analysis; Lipopolysaccharides; Reproducibility of Results; Salmonella enterica; Sensitivity and Specificity; Tongue; Bacteria (microorganisms); Negibacteria; Biomimetic Materials; Biosensing Techniques; Complex Mixtures; Conductometry; Endotoxins; Equipment Design; Equipment Failure Analysis; Lipopolysaccharides; Reproducibility of Results; Salmonella enterica; Sensitivity and Specificity; Tongue
Año:2010
Volumen:25
Número:11
Página de inicio:2470
Página de fin:2476
DOI: http://dx.doi.org/10.1016/j.bios.2010.04.004
Título revista:Biosensors and Bioelectronics
Título revista abreviado:Biosens. Bioelectron.
ISSN:09565663
CODEN:BBIOE
CAS:amino acid, 65072-01-7; protein, 67254-75-5; Complex Mixtures; Endotoxins; Lipopolysaccharides; Complex Mixtures; Endotoxins; Lipopolysaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09565663_v25_n11_p2470_Heras

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Citas:

---------- APA ----------
Heras, J.Y., Pallarola, D. & Battaglini, F. (2010) . Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin. Biosensors and Bioelectronics, 25(11), 2470-2476.
http://dx.doi.org/10.1016/j.bios.2010.04.004
---------- CHICAGO ----------
Heras, J.Y., Pallarola, D., Battaglini, F. "Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin" . Biosensors and Bioelectronics 25, no. 11 (2010) : 2470-2476.
http://dx.doi.org/10.1016/j.bios.2010.04.004
---------- MLA ----------
Heras, J.Y., Pallarola, D., Battaglini, F. "Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin" . Biosensors and Bioelectronics, vol. 25, no. 11, 2010, pp. 2470-2476.
http://dx.doi.org/10.1016/j.bios.2010.04.004
---------- VANCOUVER ----------
Heras, J.Y., Pallarola, D., Battaglini, F. Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin. Biosens. Bioelectron. 2010;25(11):2470-2476.
http://dx.doi.org/10.1016/j.bios.2010.04.004