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Abstract:

The high sensitivity that can be attained using an immunoassays coupled to a rotating bioreactor with electrochemical detection mediated by [Os(bpy)2Cl(pyCOOH)]Cl, has been verified for the detection of Trypanozoma cruzi (T. cruzi), This protozoan parasite causes Chagas disease, affecting more than 18 million people in central and south America. Antibodies in the serum sample are allowed to react immunologically with whole homogenates of the parasite as antigen that are immobilized on a rotating disk. The bound antibodies are quantified by a horseradish peroxidase (HRP) enzyme labeled second antibodies specific to human IgG in presence of hydrogen peroxide using an osmium complex [Os(bpy)2Cl(pyCOOH)]Cl as enzymatic mediators. The amperometric measurement performed at 0.00 V versus Ag/AgCl can be done within 2 min and the analysis time does not exceed 23 min. The calculated detection limits was 0.01 mIU ml-1. Reproducibility assays were made using repetitive serum of 0.182 mIU ml-1 T. cruzi specific antibody (measured as the activity of the correspondent anti-serum's enzyme conjugated); the percentage standard error was less than 5%. The amperometric immunoreactors showed significantly higher sensitivity and lower time consumed than the standard spectrophotometric detection ELISA method. © 2004 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease
Autor:Salinas, E.; Torriero, A.A.J.; Battaglini, F.; Sanz, M.I.; Olsina, R.; Raba, J.
Filiación:Department of Chemistry, National University of San Luis, Chacabuco Y Pedernera, 5700 San Luis, Argentina
INQUIMAE, University of Buenos Aires, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Biosensors; Flow system; Horseradish peroxidase; Immunoassays; Osmium; Trypanosoma cruzi; Antibodies; Bioassay; Complexation; Diseases; Enzymes; Hydrogen peroxide; Immunology; Osmium compounds; Protozoa; Spectrophotometry; Chagas disease; Horseradisg peroxidase (HRP) enzymes; Immunoassays; Serum; Bioreactors; horseradish peroxidase; human immunoglobulin; hydrogen peroxide; immobilized antibody; immunoglobulin G; parasite antibody; parasite antigen; amperometry; analytical error; antibody labeling; antigen antibody reaction; article; bioreactor; bioreactor design; Chagas disease; continuous flow reactor; enzyme immunoassay; parasite identification; quantitative analysis; reproducibility; spectrophotometry; Trypanosoma cruzi; Animals; Antibodies, Protozoan; Bioreactors; Chagas Disease; Equipment Design; Equipment Failure Analysis; Flow Injection Analysis; Humans; Immunoenzyme Techniques; Rotation; Trypanosoma cruzi; Armoracia rusticana; Protozoa; Trypanosoma cruzi
Año:2005
Volumen:21
Número:2
Página de inicio:313
Página de fin:321
DOI: http://dx.doi.org/10.1016/j.bios.2004.09.029
Título revista:Biosensors and Bioelectronics
Título revista abreviado:Biosens. Bioelectron.
ISSN:09565663
CODEN:BBIOE
CAS:hydrogen peroxide, 7722-84-1; immunoglobulin G, 97794-27-9; Antibodies, Protozoan
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09565663_v21_n2_p313_Salinas

Referencias:

  • Babkina, S.S., Medyantseva, E.P., Budnikob, H.C., Tyshlek, M.P., New variants of enzyme immunoassay of antibodies to DNA (1996) Anal. Chem., 68, pp. 3827-3831
  • Basso, B., Moretti, E.R., Dominguez, M., Culture of Trypanosoma cruzi in a monophasic medium with aeration to obtain antigens (1980) Medicina (B Aires), 40 (4), pp. 428-432
  • Basso, B., Moretti, E.R., Vottero-Cima, E., Immune response and Trypanosoma cruzi infection in Trypanosoma rangeli-immunized mice (1991) Am. J. Trop. Med. Hyg., 44 (4), pp. 413-419
  • Cai, D., Tien, M., Characterization of the oxycomplex of lignin peroxidase from phanerochaete chrysosporium: Equilibrium and kinetic study (1990) Biochemistry, 29, pp. 2085-2092
  • Carlier, Y., Pinto Dias, J.C., Ostermayer Luquetti, A., Hontebeyrie, M., Torrico, F., Truyens, C., Trypanosomiase américaine ou Maladie de Chagas (2002) Encycl. Méd. Chir., , Editions Scientifiques et médicales. Elsevier, SAS, tdr, editors. Paris 8-505-A-20:21
  • Danilowicz, C., Cortón, E., Battaglini, F., Osmium complexes bearing functional groups: Building blocks for integrated systems (1998) J. Electroanal. Chem., 445, pp. 89-94
  • Dequaire, M., Limoges, B., Moiroux, J., Savéant, J.M., Mediated electrochemistry of horseradish peroxidasa: Catalysis and inhibition (2002) J. Am. Chem. Soc., 124, pp. 240-253
  • Duane, C., Meyerhoff, M.E., Separation-free sandwich enzyme immunoassay using microporous gold electrodes and self-assembled monolayer/immobilized capture antibodies (1994) Anal. Chem., 66, pp. 1369-1375
  • Engvall, E., Enzyme immunoassay ELISA and EMIT (1980) Meth. Enzymol., 70, pp. 419-429
  • Gold, M.H., Loehr, T.M., Resonance Raman spectroscopy characterization of compound III of lignin peroxidase (1990) Biochemistry, 29, pp. 9617-9621
  • Lapierre, A., Battaglini, F., Raba, J., Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples (2001) Anal. Chim. Acta, 398, pp. 1-8
  • Lim, T., Komoda, Y., Nakamura, N., Automated detection of anti-double stranded DNA antibody in systemic lupus erythematosus serum by flow immunoassay (1999) Anal. Chem., 71, pp. 1298-1302
  • Lin, J.N., Andrade, J.D., Chang, I.N., The influence of adsorption of native and modified antibodies on their activity (1989) J. Immunol. Meth., 125, pp. 67-76
  • Liu, G.D., Yan, J.T., Shen, G.L., Yu, R.Q., Renewable amperometric immunosensor for complement 3 (C3) assay in human serum (2001) Sens. Actuators B, 80, pp. 95-100
  • Manning, P., Athey, D., McNeil, C., Homogeneous amperometric immunoassay for digoxin (1994) Anal. Lett., 27, pp. 2443-2449
  • Moncayo, A., (1993) Chagas Disease, pp. 67-75. , World Health Organization, Geneva, Tropical Disease Research. Eleventh Program Report
  • Nakajima, R., Yamazaki, I.J., The conversion of horseradish peroxidase C to a verdohemoprotein by a hydroperoxide derived enzymatically from indole-3-acetic acid and by m-nitroperoxybenzoic acid (1980) J. Biol. Chem., 255, pp. 2067-2073
  • Nakajima, R., Yamazaki, I., The mechanism of oxyperoxidase formation from ferryl peroxidase and hydrogen peroxide (1987) J. Biol. Chem., 262, pp. 2576-2581
  • Parellada, J., Narvaes, A., Lopez, M.A., Domínguez, E., Fernández, J.J., Katakis, J., Amperometric immunosensors and enzyme electrodes for environmental applications (1998) Anal. Chim. Acta, 362, pp. 47-57
  • Rodriguez-Lopez, J.N., Hernandez-Ruiz, J., Garcia-Canovas, F., Thorneley, R.N.F., Acosta, M., Arnao, M.B.J., The inactivation and catalytic pathways of horseradish peroxidase with m-chloroperoxybenzoic acid a spectrophotometric and transient kinetic study (1997) J. Biol. Chem., 272, pp. 5469-5476
  • Rodriguez-Lopez, J.N., Smith, A.T., Thorneley, R.N.F., Effect of distal cavity mutations on the binding and activation of oxygen by ferrous horseradish peroxidase (1997) J. Biol. Chem., 272, pp. 389-395
  • Santandreu, M., Cespedes, F., Alegret, S., Martinez-Fabregas, E., Amperometric immunosensors based on rigid conducting immunocomposites (1997) Anal. Chem., 69, pp. 2080-2085
  • Van Emon, J.M., Lopez Avila, V., Immunochemical methods for environmental analysis (1992) Anal. Chem., 64, pp. 79A-84A
  • Wehmeyer, K.R., Halsall, H.R., Heineman, R., Volle, C.P., Chen, I.W., Competitive heterogeneous enzyme immunoassay for digoxin with electrochemical detection (1986) Anal. Chem., 58, pp. 135-139
  • Wemhoff, G.A., Rabbany, S.Y., Kusterbeck, A.W., Ogert, R.A., Bredehorst, R., Ligter, F.S., Kinetics of antibody binding at solid-liquid interfaces in flow (1992) J. Immunol. Meth., 156, pp. 223-230
  • Wittenberg, J.B., Noble, R.W., Wittenberg, B.A., Antonini, E., Brunori, M., Wyman, J.J., Studies on the equilibrium and kinetics of the reactions of peroxidase with ligands. II. the reaction of ferroperoxidase with oxygen (1967) J. Biol. Chem., 242, pp. 626-632
  • Wu, Z.Y., Shen, G.L., Li, Z.Q., Wang, S.P., Yu, R.Q., A direct immunoassay for schistosoma japonium antibody (SjAb) in serum by piezoelectric body acoustic wave sensor (1999) Anal. Chim. Acta, 398, pp. 57-63
  • Yao, H., Jenkins, S.H., Pesce, A., Halsall, H.B., Heineman, W.R., Electrochemical homogeneous enzyme immunoassay of theophylline in hemolized, icteric, and lipemic samples (1993) Clin. Chem., 39, pp. 1423-1430

Citas:

---------- APA ----------
Salinas, E., Torriero, A.A.J., Battaglini, F., Sanz, M.I., Olsina, R. & Raba, J. (2005) . Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease. Biosensors and Bioelectronics, 21(2), 313-321.
http://dx.doi.org/10.1016/j.bios.2004.09.029
---------- CHICAGO ----------
Salinas, E., Torriero, A.A.J., Battaglini, F., Sanz, M.I., Olsina, R., Raba, J. "Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease" . Biosensors and Bioelectronics 21, no. 2 (2005) : 313-321.
http://dx.doi.org/10.1016/j.bios.2004.09.029
---------- MLA ----------
Salinas, E., Torriero, A.A.J., Battaglini, F., Sanz, M.I., Olsina, R., Raba, J. "Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease" . Biosensors and Bioelectronics, vol. 21, no. 2, 2005, pp. 313-321.
http://dx.doi.org/10.1016/j.bios.2004.09.029
---------- VANCOUVER ----------
Salinas, E., Torriero, A.A.J., Battaglini, F., Sanz, M.I., Olsina, R., Raba, J. Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease. Biosens. Bioelectron. 2005;21(2):313-321.
http://dx.doi.org/10.1016/j.bios.2004.09.029