Abstract:
Multilayer immobilization of antibody and redox polymer molecules on a gold electrode was achieved, as a strategy for the potential development of an amperometric immunosensor. The step-by-step assembly of antibiotin IgG on Os(bpy)2ClPyCH2NH poly(allylamine) redox polymer (PAH-Os) adsorbed on thiolated gold electrodes was proved by quartz crystal microbalance (QCM) and atomic force microscopy (AFM) experiments, confirming the electrochemical evidence. The increase of redox charge during the layer-by-layer deposition demonstrated that charge propagation within the layers is feasible. The multilayer structure proved to be effective for the molecular recognition of horseradish peroxidase-biotin conjugate (HRP-biotin), as confirmed by the QCM measurements and the electrocatalytic reduction current obtained upon H2O2 addition. The catalytic current resulting from PAH-Os mediation was shown to increase with the number of assembled layers. Furthermore, the inventory of IgG molecules on the supramolecular self-assembled structure and the specific and non-specific binding of HRP-biotin conjugate were confirmed by the QCM transient studies, giving information on the kinetics of IgG deposition and HRP-biotin conjugate binding to the IgG. © 2003 Elsevier B.V. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes |
Autor: | Calvo, E.J.; Danilowicz, C.; Lagier, C.M.; Manrique, J.; Otero, M. |
Filiación: | INQUIMAE, Fac. de Ciencias Exactas Y Naturales, Ciudad Universitaria, AR-1428, Buenos Aires, Argentina Fac. de Farmacia Y Bioquímica, Universidad de Buenos Aires, Junín 956, AR-1113, Buenos Aires, Argentina Fac. Cie. Bioquimicas Y F., Universidad Nacional de Rosario, Suipacha 531, AR-2000, Rosario, Argentina CONICET, Argentina Physics Department, Harvard University, 17 Oxford Street, Cambridge, MA 02138, United States
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Palabras clave: | IgG; Immunoassays; Immunoelectrodes; Redox polymer; Self-assembled; Atomic force microscopy; Deposition; Electrodes; Gold; Polymers; Self assembly; Conjugate binding; Electrocatalysis; Biosensors; antibody; avidin; biotin; gold complex; immobilized antibody; immunoglobulin G; peroxidase; polymer; adsorption; amperometric biosensor; analytic method; article; atomic force microscopy; catalysis; catalyst; chemical reaction; conjugation; crystalloid; electrochemistry; electrode; molecular recognition; oxidation reduction reaction; Antibodies; Electrochemistry; Electrodes; Gold; Immunoassay; Kinetics; Microscopy, Atomic Force; Oxidation-Reduction; Polymers; Armoracia rusticana |
Año: | 2004
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Volumen: | 19
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Número: | 10
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Página de inicio: | 1219
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Página de fin: | 1228
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DOI: |
http://dx.doi.org/10.1016/j.bios.2003.11.017 |
Título revista: | Biosensors and Bioelectronics
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Título revista abreviado: | Biosens. Bioelectron.
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ISSN: | 09565663
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CODEN: | BBIOE
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CAS: | biotin, 58-85-5; immunoglobulin G, 97794-27-9; peroxidase, 9003-99-0; Antibodies; Gold, 7440-57-5; Polymers
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09565663_v19_n10_p1219_Calvo |
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Citas:
---------- APA ----------
Calvo, E.J., Danilowicz, C., Lagier, C.M., Manrique, J. & Otero, M.
(2004)
. Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes. Biosensors and Bioelectronics, 19(10), 1219-1228.
http://dx.doi.org/10.1016/j.bios.2003.11.017---------- CHICAGO ----------
Calvo, E.J., Danilowicz, C., Lagier, C.M., Manrique, J., Otero, M.
"Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes"
. Biosensors and Bioelectronics 19, no. 10
(2004) : 1219-1228.
http://dx.doi.org/10.1016/j.bios.2003.11.017---------- MLA ----------
Calvo, E.J., Danilowicz, C., Lagier, C.M., Manrique, J., Otero, M.
"Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes"
. Biosensors and Bioelectronics, vol. 19, no. 10, 2004, pp. 1219-1228.
http://dx.doi.org/10.1016/j.bios.2003.11.017---------- VANCOUVER ----------
Calvo, E.J., Danilowicz, C., Lagier, C.M., Manrique, J., Otero, M. Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes. Biosens. Bioelectron. 2004;19(10):1219-1228.
http://dx.doi.org/10.1016/j.bios.2003.11.017