Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Multilayer immobilization of antibody and redox polymer molecules on a gold electrode was achieved, as a strategy for the potential development of an amperometric immunosensor. The step-by-step assembly of antibiotin IgG on Os(bpy)2ClPyCH2NH poly(allylamine) redox polymer (PAH-Os) adsorbed on thiolated gold electrodes was proved by quartz crystal microbalance (QCM) and atomic force microscopy (AFM) experiments, confirming the electrochemical evidence. The increase of redox charge during the layer-by-layer deposition demonstrated that charge propagation within the layers is feasible. The multilayer structure proved to be effective for the molecular recognition of horseradish peroxidase-biotin conjugate (HRP-biotin), as confirmed by the QCM measurements and the electrocatalytic reduction current obtained upon H2O2 addition. The catalytic current resulting from PAH-Os mediation was shown to increase with the number of assembled layers. Furthermore, the inventory of IgG molecules on the supramolecular self-assembled structure and the specific and non-specific binding of HRP-biotin conjugate were confirmed by the QCM transient studies, giving information on the kinetics of IgG deposition and HRP-biotin conjugate binding to the IgG. © 2003 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes
Autor:Calvo, E.J.; Danilowicz, C.; Lagier, C.M.; Manrique, J.; Otero, M.
Filiación:INQUIMAE, Fac. de Ciencias Exactas Y Naturales, Ciudad Universitaria, AR-1428, Buenos Aires, Argentina
Fac. de Farmacia Y Bioquímica, Universidad de Buenos Aires, Junín 956, AR-1113, Buenos Aires, Argentina
Fac. Cie. Bioquimicas Y F., Universidad Nacional de Rosario, Suipacha 531, AR-2000, Rosario, Argentina
CONICET, Argentina
Physics Department, Harvard University, 17 Oxford Street, Cambridge, MA 02138, United States
Palabras clave:IgG; Immunoassays; Immunoelectrodes; Redox polymer; Self-assembled; Atomic force microscopy; Deposition; Electrodes; Gold; Polymers; Self assembly; Conjugate binding; Electrocatalysis; Biosensors; antibody; avidin; biotin; gold complex; immobilized antibody; immunoglobulin G; peroxidase; polymer; adsorption; amperometric biosensor; analytic method; article; atomic force microscopy; catalysis; catalyst; chemical reaction; conjugation; crystalloid; electrochemistry; electrode; molecular recognition; oxidation reduction reaction; Antibodies; Electrochemistry; Electrodes; Gold; Immunoassay; Kinetics; Microscopy, Atomic Force; Oxidation-Reduction; Polymers; Armoracia rusticana
Año:2004
Volumen:19
Número:10
Página de inicio:1219
Página de fin:1228
DOI: http://dx.doi.org/10.1016/j.bios.2003.11.017
Título revista:Biosensors and Bioelectronics
Título revista abreviado:Biosens. Bioelectron.
ISSN:09565663
CODEN:BBIOE
CAS:biotin, 58-85-5; immunoglobulin G, 97794-27-9; peroxidase, 9003-99-0; Antibodies; Gold, 7440-57-5; Polymers
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09565663_v19_n10_p1219_Calvo

Referencias:

  • Anicet, N., Anne, A., Moiroux, J., Savéant, J.M., Electron transfer in organized assemblies of biomolecules. Construction and dynamics of avidin/biotin co-immobilized glucose oxidase/ferrocene monolayer carbon electrodes (1998) J. Am. Chem. Soc., 120, pp. 7115-7116
  • Blonder, R., Katz, E., Cohen, Y., Itzhak, N., Riklin, A., Willner, I., Application of redox enzymes for probing the antigen-antibody association at monolayer interfaces: Development of immunosensor electrodes (1996) Anal. Chem., 68, pp. 3151-3157
  • Bourdillon, C., Demaille, C., Moiroux, J., Savéant, J.M., Step-by-step immunological construction of a fully active multilayer enzyme electrode (1994) J. Am. Chem. Soc., 116, pp. 10328-10329
  • Buijs, J., Lichtenbelt, J.W., Norde, N., Lyklema, J., Adsorption of monoclonal IgGs and their F(ab′)2 fragments onto polymeric surfaces (1995) Colloids Surf. B Biointer., 5, pp. 11-23
  • Bustamante, C., Vesenka, J., Tang, C.L., Rees, W., Guthold, M., Séller, R., Circular DNA molecules imaged in air by scanning force microscopy (1992) Biochemistry, 31, pp. 22-28
  • Calvo, E.J., Danilowicz, C., Etchenique, R., Measurement of viscoelastic changes at electrodes modified with redox hydrogels with a quartz crystal device (1995) J. Chem. Soc. Farad. Trans., 91, pp. 4083-4091
  • Calvo, E.J., Battaglini, F., Danilowicz, C., Wolosiuk, A., Otero, M., Layer-by-layer electrostatic deposition of biomolecules on surfaces for molecular recognition, redox mediation and signal generation (2000) Faraday Discuss. Chem. Soc., 116, pp. 47-65
  • Calvo, E.J., Etchenique, R., Pietrasanta, L., Wolosiuk, A., Danilowicz, C., Layer-by-layer self-assembly of glucose oxidase and Os(bpy) 2ClPyCH2NH-poly(allylamine) bioelectrode (2001) Anal. Chem., 73, pp. 1161-1168
  • Calvo, E.J., Danilowicz, C., Forzani, E., Wolosiuk, A., Otero, M., Layered protein films: Quartz crystal resonator frequency and admittance analysis (2003) Biomolecular Films: Design, Function and Applications, , Rusling, J.F. (Ed.), Marcel Dekker, New York (Chapter 7)
  • Caruso, F., Rodda, E., Furlong, D.N., Orientational aspects of antibody immobilization and immunological activity on quartz crystal microbalance electrodes (1996) J. Colloid Interf. Sci., 178, pp. 104-115
  • Caruso, F., Niikura, K., Neil Furlong, D., Okahata, Y., Assembly of alternating polyelectrolyte and protein multilayer films for immunosensing (1997) Langmuir, 13, pp. 3427-3433
  • Caruso, F., Neil Furlong, D., Ariga, K., Ichinose, I., Kunitake, T., Characterization of polyelectrolyte-protein multilayer films by atomic force microscopy, scanning electron microscopy and Fourier transform infrared reflection-absorption spectroscopy (1998) Langmuir, 14, pp. 4559-4565
  • Danilowicz, C., Corton, E., Battaglini, F., Osmium complexes bearing functional groups: Building blocks for integrated chemical systems (1998) J. Electroanal. Chem., 445, pp. 89-94
  • Danilowicz, C., Manrique, J.M., A new self-assembled modified electrode for competitive immunoassay (1999) Electrochem. Commun., 1, pp. 22-25
  • Decher, G., Fuzzy nanoassemblies: Toward layered polymeric multicomposites (1997) Science, 277, pp. 1232-1237
  • Duan, C., Meyerhoff, M.E., Separation-free sandwich enzyme immunoassays using microporous gold electrodes and self-assembled monolayer/immobilized capture antibodies (1994) Anal. Chem., 66, pp. 1369-1377
  • Forzani, E.S., Solís, V.M., Calvo, E.J., Electrochemical behavior of polyphenol oxidase immobilized in self-assembled structures layer by layer with cationic polyallylamine (2000) Anal. Chem., 72, pp. 5300-5307
  • Gregg, B.A., Heller, A., Electrical connection of enzyme redox centers to electrodes (1992) J. Phys. Chem., 96, pp. 3579-3587
  • Heller, A., Electrical wiring of redox enzymes (1990) Acc. Chem. Res., 23, pp. 128-134
  • Hidalgo-Alvarez, R., Galisteo-Gonzalez, F., The adsorption characteristics of immunoglobulins (1995) Hetero. Chem. Rev., 2, pp. 249-268
  • Hodak, J., Etchenique, R., Calvo, E.J., Singhal, K., Bartlett, P.N., Layer by layer self assembly of Glucose oxidase with a poly(allylamine) ferrocene redox mediator (1997) Langmuir, 13, pp. 2708-2716
  • Lu, B., Iwuoha, E.I., Smyth, M.R., O'Kennedy, R., Development of an electrically wired amperometric immunosensor for the determination of biotin based on a non-diffusional redox osmium polymer film containing an antibody to the enzyme label horseradish peroxidase (1997) Anal. Chim. Acta, 345, pp. 59-66
  • Lvov, Y., Essler, F., Decher, G., Combination of polycation/polyanion self assembly and Langmuir-Blodgett transfer for the construction of superlattice films (1993) J. Phys. Chem., 97, pp. 13773-13777
  • Lvov, Y., Ariga, K., Ichinose, I., Kunitake, T., Assembly of multicomponent protein films by means of electrostatic layer-by-layer adsorption (1995) J. Am. Chem. Soc., 117, pp. 6117-6123
  • Muramatsu, H., Dicks, J.M., Tamiya, E., Karube, I., Piezoelectric crystal biosensor modified with Protein a for determination of immunoglobulins (1997) Anal. Chem., 59, pp. 2760-2763
  • Purvis, D.R., Pollard-Knight, D., Lowe, P.A., Biosensors based on evanescent waves (1998) Commercial Biosensors, pp. 165-224. , Ramsay, E. (Ed.), John Wiley & Sons Inc., New York
  • Rickert, J., Brecht, A., Göpel, W., QCM operation in liquids: Constant sensitivity during formation of extended protein multilayers by affinity (1997) Anal. Chem., 69, pp. 1441-1448
  • Rusling, J.F., Enzyme bioelectrochemistry in cast biomembrane like films (1998) Acc. Chem. Res., 31, p. 363
  • Sauerbrey, G., Verwendung von schwingquarzen zur wägung düner schechten and zur mikrowägung (1959) Z. Phys., 155, pp. 206-222
  • Suh, J., Paik, H., Hwang, B.K., Ionisation of poly(ethylenimine) and poly(allylamine) at various pH's (1994) Bioorg. Chem., 22, pp. 318-327
  • Ward, M.D., Buttry, D.A., In situ interfacial mass detection with piezoelectric transducers (1990) Science, 249, pp. 1000-1007

Citas:

---------- APA ----------
Calvo, E.J., Danilowicz, C., Lagier, C.M., Manrique, J. & Otero, M. (2004) . Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes. Biosensors and Bioelectronics, 19(10), 1219-1228.
http://dx.doi.org/10.1016/j.bios.2003.11.017
---------- CHICAGO ----------
Calvo, E.J., Danilowicz, C., Lagier, C.M., Manrique, J., Otero, M. "Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes" . Biosensors and Bioelectronics 19, no. 10 (2004) : 1219-1228.
http://dx.doi.org/10.1016/j.bios.2003.11.017
---------- MLA ----------
Calvo, E.J., Danilowicz, C., Lagier, C.M., Manrique, J., Otero, M. "Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes" . Biosensors and Bioelectronics, vol. 19, no. 10, 2004, pp. 1219-1228.
http://dx.doi.org/10.1016/j.bios.2003.11.017
---------- VANCOUVER ----------
Calvo, E.J., Danilowicz, C., Lagier, C.M., Manrique, J., Otero, M. Characterization of self-assembled redox polymer and antibody molecules on thiolated gold electrodes. Biosens. Bioelectron. 2004;19(10):1219-1228.
http://dx.doi.org/10.1016/j.bios.2003.11.017