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Abstract:

Direct electrical communication between a new acrylamide-acrylic acid ferrocence copolymer and the enzyme cofactor FADH2 of glucose oxidase is demonstrated. Subsequent catalysis for the anaerobic oxidation of glucose at the co-reticulated enzyme-polymer-modified electrode is shown for polymer-coated glassy carbon and for a composite carbon-paste electrode. A theoretical framework for the kinetic analysis of the reagentless amperometric enzyme electrode is outlined and the model is validated with experimental results. Based on this analysis, kinetic data for the ping-pong glucose oxidase mechanism are discussed for the immobilized enzyme. A comparison of immobilized enzyme electrodes is presented with a redox polymer and soluble substituted-ferrocene redox mediator of similar formal redox potential.

Registro:

Documento: Artículo
Título:Enzyme catalysis at hydrogel-modified electrodes with redox polymer mediator
Autor:Calvo, E.J.; Danilowicz, C.; Diaz, L.
Filiación:Departamento de Química Inorgánica, Analítica y Química Física, Universidad de Buenos Aires, Ciudad Universitaria, AR-1428 Buenos Aires, Argentina
Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junin 956, AR-1113 Buenos Aires, Argentina
Argentine Science Research Council (CONICET)
Año:1993
Volumen:89
Número:2
Página de inicio:377
Página de fin:384
DOI: http://dx.doi.org/10.1039/FT9938900377
Título revista:Journal of the Chemical Society, Faraday Transactions
ISSN:09565000
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09565000_v89_n2_p377_Calvo

Referencias:

  • Albery, W.J., Boutelle, M.G., Durrant, S.L.T., Fillenz, M., Hopkins, A.R., Mangol, B.P., (1990) Philos. Trans. R. Soc. London, A, 333, p. 49
  • Heller, A., (1992) J. Phys. Chem., 96, p. 3579
  • Foulds, N.C., Lowe, C.R., (1988) Anal. Chem., 60, p. 2473
  • Degani, Y., Heller, A., (1987) J. Phys. Chem., 91, p. 1285
  • Heller, A., Degani, Y., (1988) Redox Chemistry and Interfacial Behaviour of Biological Molecules, p. 151. , G. Dryhurst and K. Niki, Plenum, New York
  • Degani, Y., Heller, A., (1988) J. Am. Chem. Soc., 110, p. 2615
  • Schuhmann, W., Ohara, T.J., Schmidt, H.L., Heller, A., (1991) J. Am. Chem. Soc., 113, p. 1394
  • Bartlett, P.N., Whitaker, R.G., (1987) J. Chem. Soc., Chem. Commun., p. 1603
  • Bartlett, P.N., Bradford, V.Q., Whitaker, R.G., (1991) Talanta, 38, p. 57
  • Heller, A., (1990) Acc. Chem. Res., 23, p. 128
  • Degani, Y., Heller, A., (1989) J. Am. Chem. Soc., 111, p. 2357
  • Pishko, M.V., Katakis, I., Linquist, S.E., Ye, L., Gregg, B.A., Heller, A., (1990) Angew. Chem., Int. Ed. Engl., 29, p. 82
  • Gregg, B.A., Heller, A., (1990) Anal. Chem., 62, p. 258
  • Gregg, B.A., Heller, A., (1991) J. Phys. Chem., 95, pp. 5970-5976
  • Pishko, M.V., Michael, A.C., Heller, A., (1991) Anal. Chem., 63, p. 2268
  • Jönsson, G., Gorton, L., Pettersson, L., (1989) Electroanalysis, 1, p. 49
  • Inagaki, T., Lee, H.S., Skotheim, T.A., Okamoto, Y., (1989) J. Chem. Soc., Chem. Commun., p. 1181
  • Hale, P.D., Inagaki, T., Karan, H.I., Okamoto, Y., Skotheim, T.A., (1989) J. Am. Chem. Soc., 111, p. 3482
  • Gorton, L., Karan, H.I., Hale, P.D., Inagaki, T., Okamoto, Y., Skotheim, T.A., (1990) Anal. Chim. Acta, 228, p. 23
  • Hale, P.D., Inagaki, T., Lee, H.S., Karan, H.I., Okamoto, Y., Skotheim, T.A., (1990) Anal. Chim. Acta, 228, p. 31
  • Hale, P.D., Boguslavsky, L.I., Inagaki, T., Karan, H.I., Lee, H.S., Skotheim, T.A., Okamoto, Y., (1991) Anal. Chem., 63, p. 677
  • Inagaki, T., Lee, H.S., Hale, P.D., Skotheim, T.A., Okamoto, Y., (1989) Macromolecules, 22, p. 4641
  • Lange, M.A., Chambers, J.Q., (1985) Anal. Chim. Acta, 175, p. 89
  • Willner, I., Riklin, A., Lapidot, N., (1990) J. Am. Chem. Soc., 112, p. 6438
  • (1992) Polyelectrolyte Gels: Properties, Preparation and Applications, , R. Harland and R. Prod'homme, ACS Symp. Ser. 480, American Chemical Society, Washington D.C
  • (1976) Methods in Enzymology, 44, p. 54. , K. Mosbach
  • Avrameas, S., (1985) Affinity Chromatography: A Practical Approach, p. 64. , P. O. G. Dean, W. S. Johnson and F. A. Middle, IRL Press, Oxford
  • Surridge, N.A., Jernigan, J.C., Dalton, E.F., Buck, R.P., Watanabe, M., Zhang, H., Pinkerton, M., Murray, R.W., (1989) Faraday Discuss. Chem. Soc., 88, p. 1
  • Van Alsten, J., (1991) Macromolecules, 24, p. 5320
  • Hubbard, A.T., Anson, F.C., (1970) Electroanalytical Chemistry, p. 129. , A. J. Bard, Marcel Dekker, New York, 4
  • Albery, W.J., Boutelle, M.G., Colby, P.J., Hillman, A.R., (1982) J. Electroanal. Chem., 133, p. 135
  • Fersht, A., (1985) Enzyme Structure and Mechanism, , Freeman, New York, 2nd edn
  • Bright, H.J., Porter, D.J.T., (1975) The Enzymes, 12. , P. Boyer, Academic Press, New York, ch. 7, p. 421
  • Gough, D.A., Leypoldt, J.K., (1981) Appl. Biochem. Bioeng., 3, p. 175
  • Liaudet, E., Battaglini, F., Calvo, E.J., (1990) J. Electroanal. Chem., 293, p. 55
  • Bartlett, P.N., Whitaker, R.G., (1989) Anal. Chem., 61, p. 2803
  • Peerce, P.J., Bard, A.J., (1980) J. Electroanal. Chem., 114, p. 89
  • Cass, A.E.G., Davis, G., Francis, G.D., Hill, H.A.O., Aston, W.J., Higgins, I.J., Plotkin, E.V., Turner, A.P.F., (1984) Anal. Chem., 56, p. 667
  • Sharp, M., Petersson, M., Edström, K., (1979) J. Electroanal. Chem., 95, p. 123
  • O'Connel, K.M., Waldner, E., Roullier, L., Laviron, E., (1984) J. Electroanal. Chem., 162, p. 77
  • Saveant, J.M., (1991) J. Electroanal. Chem., 302, p. 91
  • Battaglini, F., Calvo, E.J., J. Electroanal. Chem., , submitt
  • Albery, W.J., Bartlett, P.N., Driscoll, B.J., Lennox, R.B., (1992) J. Electroanal. Chem., 323, p. 77
  • Battaglini, F., Calvo, E.J., (1992) Anal. Chim. Acta, 258, p. 151
  • Chambers, J.A., Walton, N.J., (1988) J. Electroanal. Chem., 250, p. 417
  • Laidler, K.J., Bunting, P.S., (1980) Methods in Enzymology, 64, p. 227
  • Sundaram, P.V., Twedale, A., Laidler, K.J., (1970) Can. J. Chem., 48, p. 1498
  • Horvath, C., Engasser, J.M., (1974) Biotechnol. Bioeng., 16, p. 909
  • Frew, J.E., Harmer, M.A., Hill, H.A.O., Libor, S.I., (1986) J. Electroanal. Chem., 201, p. 1

Citas:

---------- APA ----------
Calvo, E.J., Danilowicz, C. & Diaz, L. (1993) . Enzyme catalysis at hydrogel-modified electrodes with redox polymer mediator. Journal of the Chemical Society, Faraday Transactions, 89(2), 377-384.
http://dx.doi.org/10.1039/FT9938900377
---------- CHICAGO ----------
Calvo, E.J., Danilowicz, C., Diaz, L. "Enzyme catalysis at hydrogel-modified electrodes with redox polymer mediator" . Journal of the Chemical Society, Faraday Transactions 89, no. 2 (1993) : 377-384.
http://dx.doi.org/10.1039/FT9938900377
---------- MLA ----------
Calvo, E.J., Danilowicz, C., Diaz, L. "Enzyme catalysis at hydrogel-modified electrodes with redox polymer mediator" . Journal of the Chemical Society, Faraday Transactions, vol. 89, no. 2, 1993, pp. 377-384.
http://dx.doi.org/10.1039/FT9938900377
---------- VANCOUVER ----------
Calvo, E.J., Danilowicz, C., Diaz, L. Enzyme catalysis at hydrogel-modified electrodes with redox polymer mediator. 1993;89(2):377-384.
http://dx.doi.org/10.1039/FT9938900377