Artículo

García, C.C.; Brousse, B.N.; Carlucci, M.J.; Moglioni, A.G.; Alho, M.M.; Moltrasio, G.Y.; D'Accorso, N.B.; Damonte, E.B. "Inhibitory effect of thiosemicarbazone derivatives on Junin virus replication in vitro" (2003) Antiviral Chemistry and Chemotherapy. 14(2):99-105
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Abstract:

The inhibitory effect of several thiosemicarbazones (TSCs), synthesized from aromatic ketones and terpenones, and their heterocyclic thiadiazoline (TDZ) derivatives, was investigated against Junin virus (JUNV), an arenavirus agent of Argentine haemorrhagic fever. From the 25 compounds tested, six compounds belonging to the TSC group were found to be selective inhibitors of JUNV, with EC 50 values determined by a virus yield inhibition assay in the range 3.4-12.5 μM, and selectivity indices greater than 10. By contrast, most of the TDZs obtained by heterocyclization of the TSCs were not active against JUNV. No conclusive structure-activity relationships could be established but systematically higher activity was associated to TSCs derived from aromatic ketones. The mode of action of one of the most active compound, the 3,4-dihydronaphtalen-1(2H)one thiosemicarbazone (tetralone thiosemicarbazone), was studied further. This TSC lacked virucidal effects on JUNV virions. Results from time of addition experiments and viral protein expression assays suggest that tetralone thiosemicarbazone inhibited a late stage in the replicative cycle of JUNV.

Registro:

Documento: Artículo
Título:Inhibitory effect of thiosemicarbazone derivatives on Junin virus replication in vitro
Autor:García, C.C.; Brousse, B.N.; Carlucci, M.J.; Moglioni, A.G.; Alho, M.M.; Moltrasio, G.Y.; D'Accorso, N.B.; Damonte, E.B.
Filiación:Laboratorio de Virología, Departamento de Quimica Biologica, Ciudad Universitaria, Buenos Aires, Argentina
Palabras clave:Antiviral; Arenavirus; Haemorrhagic fever; Junin virus; Thiosemicarbazone derivatives; 1 tetralone derivative; ketone; terpenoid; thiazolidine derivative; thiosemicarbazone; animal cell; antiviral activity; Arenavirus; article; cyclization; hemorrhagic fever; Junin virus; nonhuman; priority journal; protein expression; structure activity relation; virion; virus infection; virus inhibition; virus replication
Año:2003
Volumen:14
Número:2
Página de inicio:99
Página de fin:105
DOI: http://dx.doi.org/10.1177/095632020301400205
Título revista:Antiviral Chemistry and Chemotherapy
Título revista abreviado:Antiviral Chem. Chemother.
ISSN:09563202
CODEN:ACCHE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09563202_v14_n2_p99_Garcia

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Citas:

---------- APA ----------
García, C.C., Brousse, B.N., Carlucci, M.J., Moglioni, A.G., Alho, M.M., Moltrasio, G.Y., D'Accorso, N.B.,..., Damonte, E.B. (2003) . Inhibitory effect of thiosemicarbazone derivatives on Junin virus replication in vitro. Antiviral Chemistry and Chemotherapy, 14(2), 99-105.
http://dx.doi.org/10.1177/095632020301400205
---------- CHICAGO ----------
García, C.C., Brousse, B.N., Carlucci, M.J., Moglioni, A.G., Alho, M.M., Moltrasio, G.Y., et al. "Inhibitory effect of thiosemicarbazone derivatives on Junin virus replication in vitro" . Antiviral Chemistry and Chemotherapy 14, no. 2 (2003) : 99-105.
http://dx.doi.org/10.1177/095632020301400205
---------- MLA ----------
García, C.C., Brousse, B.N., Carlucci, M.J., Moglioni, A.G., Alho, M.M., Moltrasio, G.Y., et al. "Inhibitory effect of thiosemicarbazone derivatives on Junin virus replication in vitro" . Antiviral Chemistry and Chemotherapy, vol. 14, no. 2, 2003, pp. 99-105.
http://dx.doi.org/10.1177/095632020301400205
---------- VANCOUVER ----------
García, C.C., Brousse, B.N., Carlucci, M.J., Moglioni, A.G., Alho, M.M., Moltrasio, G.Y., et al. Inhibitory effect of thiosemicarbazone derivatives on Junin virus replication in vitro. Antiviral Chem. Chemother. 2003;14(2):99-105.
http://dx.doi.org/10.1177/095632020301400205