Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

A novel series of DL-galactan hybrids extracted from the red seaweed Gymnogongrus torulosus, was evaluated for its in vitro antiviral properties against herpes simplex virus type 2 (HSV-2) and dengue virus 2 (DEN-2). These compounds were very active against both viruses with inhibitory concentration 50% (IC50) values in the range 0.6-16 μg/ml for HSV-2 and 0.19-1.7 μg/ml for DEN-2, respectively, as determined in a virus plaque reduction assay in Vero cells. The DL-galactans lacked of cytotoxic effects, on stationary as well as on actively dividing cells, and anticoagulant properties. Some of the compounds showed a variable level of direct inactivating effect on both virions, with virucidal concentration 50% values exceeding the IC50S obtained by plaque reduction assay. Full inhibitory activity was achieved when the galactans were present during virus adsorption period, suggesting that the mode of action of these compounds is an interference in the binding of the surface envelope glycoprotein with the cell receptor.

Registro:

Documento: Artículo
Título:Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus
Autor:Pujol, C.A.; Estevez, J.M.; Carlucci, M.J.; Ciancia, M.; Cerezo, A.S.; Damonte, E.B.
Filiación:Laboratorio de Virologia, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Quimica Organica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Antiviral; Dengue virus; DL-glactans; Herpes simplex virus; Seaweed; Sulfated polysaccharide; antivirus agent; carrageenan; disaccharide; galactan; polysaccharide; virus envelope protein; virus glycoprotein; animal cell; anticoagulation; antiviral activity; article; controlled study; Dengue virus; drug cytotoxicity; drug isolation; drug mechanism; drug potency; gymnogongrus torulosus; Herpes simplex virus 2; in vitro study; internalization; nonhuman; priority journal; seaweed; Vero cell; virus adsorption; virus inhibition; virus plaque
Año:2002
Volumen:13
Número:2
Página de inicio:83
Página de fin:89
DOI: http://dx.doi.org/10.1177/095632020201300202
Título revista:Antiviral Chemistry and Chemotherapy
Título revista abreviado:Antiviral Chem. Chemother.
ISSN:09563202
CODEN:ACCHE
CAS:carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; galactan, 39300-87-3, 9037-55-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09563202_v13_n2_p83_Pujol

Referencias:

  • Baba, M., Snoeck, R., Pauwels, R., De Clercq, E., Sulfated polysaccharides are potent and selective inhibitors of various enveloped viruses, including herpes simplex virus, cytomegalovirus, vesicular stomatitis virus and human immunodeficiency virus (1988) Antimicrobial Agents and Chemotherapy, 32, pp. 1742-1745
  • Cáceres, P.J., Carlucci, M.J., Damonte, E.B., Matsuhiro, B., Zúñiga, E.A., Carrageenans from Chilean samples of Stenogramme interrupta (Phyllophoraceae): Structural analysis and biological activity (2000) Phytochemistry, 53, pp. 81-86
  • Carlucci, M.J., Pujol, C.A., Ciancia, M., Noseda, M.D., Matulewicz, M.C., Damonte, E.B., Cerezo, A.S., Antiherpetic and anticoagulant properties of carrageenans from the red seaweed Gigartina skottsbergii and their cyclized derivatives: Correlation between structure and biological activity (1997) International Journal of Biological Macromolecules, 20, pp. 97-105
  • Carlucci, M.J., Ciancia, M., Matulewicz, M.C., Cerezo, A.S., Damonte, E.B., Antiherpetic activity and mode of action of natural carrageenans of diverse structural types (1999) Antiviral Research, 43, pp. 93-102
  • Chen, Y., Maguire, T., Hileman, R.E., Fromm, J.R., Esko, J.D., Linhardt, R.J., Marks, R.M., Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate (1997) Nature Medicine, 3, pp. 866-871
  • Cribier, B., Schmitt, C., Kirn, A., Stoll-Keller, F., Inhibition of hepatitis C virus adsorption to peripheral blood mononuclear cells by dextran sulfate (1998) Archives of Virology, 143, pp. 375-379
  • Duckworth, M., Yaphe, W., The structure of agar. Part I. Fractionation of a complex mixmre of polysaccharides (1971) Carbohydrate Research, 16, pp. 189-197
  • Estevez, J.M., Ciancia, M., Cerezo, A.S., DL-galactan hybrids and agarans from gametophytes of the red seaweed Gymnogongrus torulosus (2001) Carbohydrate Research, 331, pp. 27-41
  • González, M.E., Alarcón, B., Carrasco, L., Polysaccharides as antiviral agents: Antiviral activity of carrageenan (1987) Antimicrobial Agents and Chemotherapy, 31, pp. 1388-1393
  • Gubler, D.J., Dengue and dengue hemorrhagic fever (1998) Clinical Microbiology Reviews, 11, pp. 480-496
  • Hosoya, M., Balzarini, J., Shigeta, S., De Clercq, E., Differential inhibitory effects of sulfated polysaccharides and polymers on the replication of various myxoviruses and retroviruses, depending on the composition of the target amino acid sequences of the viral envelope glycoproteins (1991) Antimicrobial Agents and Chemotherapy, 35, pp. 2515-2520
  • Igarashi, A., Impact of dengue virus infection and its control (1997) FEMS Immunology and Medical Microbiology, 18, pp. 291-300
  • Nakashima, H., Kido, Y., Kobayashi, N., Motoki, Y., Neushul, M., Yamamoto, N., Purification and characterization of an avian myeloblastosis and human immunodeficiency virus reverse transcriptase inhibitor sulfated polysaccharide extracted from sea algae (1987) Antimicrobial Agents and Chemotherapy, 31, pp. 1524-1528
  • Putnak, J.R., Kanesa-Thasan, N., Innis, B.L., A putative cellular receptor for dengue viruses (1997) Nature Medicine, 3, pp. 828-829
  • Stortz, C.A., Cerezo, A.S., Novel findings in carrageenans, agaroids and "hybrids" red seaweed galactans (2000) Currents Topics in Phytochemistry, 4, pp. 121-134
  • Tal-Singer, R., Peng, C., Ponce de Leon, M., Abrams, W.R., Banfield, B.W., Tufaro, F., Cohen, G.H., Eisenberg, R.J., Interaction of herpes simplex virus glycoprotein gC with mammalian cell surface molecules (1995) Journal of Virology, 69, pp. 4471-4483
  • Thullier, P., Demangel, C., Bedouelle, H., Mégret, F., Jouan, A., Deubel, V., Mazié, J.C., Lafaye, P., Mapping of a dengue virus neutralizing epitope critical for the infectivity of all serotypes: Insight into the neutralization mechanism (2001) Journal of General Virology, 82, pp. 1885-1892
  • Trybala, E., Bergstrom, T., Svennerholm, B., Jeansson, S., Glorioso, J.C., Olofsson, S., Localization of a functional site of herpes simplex virus type 1 glycoprotein C involved in binding to cell surface heparan sulfate (1994) Journal of General Virology, 75, pp. 743-752
  • Witvrouw, M., De Clercq, E., Sulfated polysaccharides extracted from sea algae as potential antiviral drugs (1997) General Pharmacology, 29, pp. 497-511
  • Witvrouw, M., Desmyter, J., De Clercq, E., Antiviral portrait series: 4. Polysulfates as inhibitors of HIV and other enveloped viruses (1994) Antiviral Chemistry & Chemotherapy, 5, pp. 345-359
  • WuDunn, D., Spear, P.G., Initial interaction of herpes simplex virus with cells is binding to heparan sulfate (1989) Journal of Virology, 63, pp. 52-58
  • Yamada, T., Ogamo, A., Saito, T., Uchiyama, H., Nakagawa, Y., Preparation of O-acylated low-molecular-weight carrageenans with potent anti-HIV activity and low anticoagulant effect (2000) Carbohydrate Polymers, 41, pp. 115-120

Citas:

---------- APA ----------
Pujol, C.A., Estevez, J.M., Carlucci, M.J., Ciancia, M., Cerezo, A.S. & Damonte, E.B. (2002) . Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus. Antiviral Chemistry and Chemotherapy, 13(2), 83-89.
http://dx.doi.org/10.1177/095632020201300202
---------- CHICAGO ----------
Pujol, C.A., Estevez, J.M., Carlucci, M.J., Ciancia, M., Cerezo, A.S., Damonte, E.B. "Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus" . Antiviral Chemistry and Chemotherapy 13, no. 2 (2002) : 83-89.
http://dx.doi.org/10.1177/095632020201300202
---------- MLA ----------
Pujol, C.A., Estevez, J.M., Carlucci, M.J., Ciancia, M., Cerezo, A.S., Damonte, E.B. "Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus" . Antiviral Chemistry and Chemotherapy, vol. 13, no. 2, 2002, pp. 83-89.
http://dx.doi.org/10.1177/095632020201300202
---------- VANCOUVER ----------
Pujol, C.A., Estevez, J.M., Carlucci, M.J., Ciancia, M., Cerezo, A.S., Damonte, E.B. Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus. Antiviral Chem. Chemother. 2002;13(2):83-89.
http://dx.doi.org/10.1177/095632020201300202