Abstract:
Fifteen antiretroviral Zn-finger active compounds with diverse chemical structures, including azoic compounds, hydrazide derivatives, disulphide-based reagents and others were screened in vitro against Junin virus (JUNV), the aetiological agent of Argentine haemorrhagic fever, by a virus yield inhibition assay in Vero cells. Cytotoxicity was evaluated simultaneously by the MTT method. Of the compounds, three were totally inactive as antivirals, nine presented moderate anti JUNV-activity and three were truly active with EC50 (effective concentration 50%) values in the range 6.5-9.3 μM and with selectivity indices greater than 10. The most active inhibitors, named NSC20625, 3-7 and 2-71, demonstrated a broad range of action against arenaviruses, including several attenuated and pathogenic strains of JUNV as well as the antigenically related Tacaribe virus (TACV) and Pichinde virus (PICV). The direct treatment of JUNV and TACV virions with the compounds showed two types of behaviour: the aromatic disulphide NSC20625 was a very potent virucidal agent, whereas the other two compounds exhibited moderate or negligible virus-inactivating properties.
Registro:
Documento: |
Artículo
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Título: | Antiviral and virucidal activities against arenaviruses of zinc-finger active compounds |
Autor: | García, C.C.; Candurra, N.A.; Damonte, E.B. |
Filiación: | Laboratorio de Virología, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina
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Palabras clave: | Arenavirus; Junin virus; Tacaribe virus; Virucidal activity; Zn-finger inhibitors; a 15073; a 19315; a 2759; a 9660; antiretrovirus agent; hydrazide derivative; nsc 20625; nsc 2065; nsc 4493; nsc 624151; nsc 624152; protein inhibitor; ribavirin; s 3176; unclassified drug; virus protein; zinc finger protein; animal cell; antiviral activity; Arenavirus; article; controlled study; drug cytotoxicity; drug selectivity; drug structure; Junin virus; nonhuman; Pichinde arenavirus; priority journal; Vero cell; virion |
Año: | 2000
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Volumen: | 11
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Número: | 3
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Página de inicio: | 231
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Página de fin: | 237
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DOI: |
http://dx.doi.org/10.1177/095632020001100306 |
Título revista: | Antiviral Chemistry and Chemotherapy
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Título revista abreviado: | Antiviral Chem. Chemother.
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ISSN: | 09563202
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CODEN: | ACCHE
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CAS: | ribavirin, 36791-04-5
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09563202_v11_n3_p231_Garcia |
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Citas:
---------- APA ----------
García, C.C., Candurra, N.A. & Damonte, E.B.
(2000)
. Antiviral and virucidal activities against arenaviruses of zinc-finger active compounds. Antiviral Chemistry and Chemotherapy, 11(3), 231-237.
http://dx.doi.org/10.1177/095632020001100306---------- CHICAGO ----------
García, C.C., Candurra, N.A., Damonte, E.B.
"Antiviral and virucidal activities against arenaviruses of zinc-finger active compounds"
. Antiviral Chemistry and Chemotherapy 11, no. 3
(2000) : 231-237.
http://dx.doi.org/10.1177/095632020001100306---------- MLA ----------
García, C.C., Candurra, N.A., Damonte, E.B.
"Antiviral and virucidal activities against arenaviruses of zinc-finger active compounds"
. Antiviral Chemistry and Chemotherapy, vol. 11, no. 3, 2000, pp. 231-237.
http://dx.doi.org/10.1177/095632020001100306---------- VANCOUVER ----------
García, C.C., Candurra, N.A., Damonte, E.B. Antiviral and virucidal activities against arenaviruses of zinc-finger active compounds. Antiviral Chem. Chemother. 2000;11(3):231-237.
http://dx.doi.org/10.1177/095632020001100306