Abstract:
A natural brassinosteroid and a series of synthetic derivatives were found to be good inhibitors of herpes simplex virus type 1 (HSV-1) and arenavirus replication in cell culture. The synthetic compounds tested were analogues of the 24(S) ethylbrassinone. Compounds (22R,23R,24S)-2α, 3α,5α,22,23-pentahydroxy-stigmastan-6-one and (22R,23R,24S)-3β-bromo-5α,22,23-trihydroxy stigm-astan-6-one were cytotoxic at concentrations of 20-40 μM. (22S,23S,24S)-2α,3α,22,23-tetrahydroxy-5α,stigmastan-6-one, (22R,23R,24S)-3β-acetoxy-22,23-dihydroxy-5α-cholestan-6-one, (22S,23S,24S)-3β-bromo-22,23-dihydroxy-5α-chol-estan-6-one and (22S,23S,24S)-3β-bromo-5α,22,23-trihydroxy-stigmastan-6-one were the most active of the series against HSV-1, with selectivity index (SI) values (CC50/EC50) ranging from 10.6 to 16.5. The majority of the compounds were potent inhibitors of arenaviruses, (22S,23S,24S)-3β-bromo-5α,22,23-trihydroxy-stig-mastan-6-one being the most active, with SI values of 307.8 and 692.5 for Tacaribe and Junin viruses, respectively. The antiviral activity of brassinosteroid derivatives was not because of direct inactivation; time-of-addition experiments suggested that a late step in HSV-1 multiplication was affected, whereas arenaviruses remained susceptible to the compounds throughout the replicative cycle.
Registro:
Documento: |
Artículo
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Título: | Antiviral effect of brassinosteroids against herpes virus and arenaviruses |
Autor: | Wachsman, M.B.; López, E.M.F.; Ramirez, J.A.; Galagovsky, L.R.; Coto, C.E. |
Filiación: | Laboratorio de Virología, Depto. de Quim. Biológica, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina Depto. de Quim. Orgánica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
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Palabras clave: | Arenaviruses; Brassinosteroids; Herpes simplex virus type 1; Junin virus; aciclovir; antivirus agent; brassinosteroid; animal cell; antiviral activity; Arenavirus; article; controlled study; drug cytotoxicity; Herpes simplex virus 1; Herpes virus; Junin virus; nonhuman; priority journal; Vero cell; virogenesis; virus replication |
Año: | 2000
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Volumen: | 11
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Número: | 1
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Página de inicio: | 71
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Página de fin: | 77
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DOI: |
http://dx.doi.org/10.1177/095632020001100107 |
Título revista: | Antiviral Chemistry and Chemotherapy
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Título revista abreviado: | Antiviral Chem. Chemother.
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ISSN: | 09563202
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CODEN: | ACCHE
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CAS: | aciclovir, 59277-89-3
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09563202_v11_n1_p71_Wachsman |
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Citas:
---------- APA ----------
Wachsman, M.B., López, E.M.F., Ramirez, J.A., Galagovsky, L.R. & Coto, C.E.
(2000)
. Antiviral effect of brassinosteroids against herpes virus and arenaviruses. Antiviral Chemistry and Chemotherapy, 11(1), 71-77.
http://dx.doi.org/10.1177/095632020001100107---------- CHICAGO ----------
Wachsman, M.B., López, E.M.F., Ramirez, J.A., Galagovsky, L.R., Coto, C.E.
"Antiviral effect of brassinosteroids against herpes virus and arenaviruses"
. Antiviral Chemistry and Chemotherapy 11, no. 1
(2000) : 71-77.
http://dx.doi.org/10.1177/095632020001100107---------- MLA ----------
Wachsman, M.B., López, E.M.F., Ramirez, J.A., Galagovsky, L.R., Coto, C.E.
"Antiviral effect of brassinosteroids against herpes virus and arenaviruses"
. Antiviral Chemistry and Chemotherapy, vol. 11, no. 1, 2000, pp. 71-77.
http://dx.doi.org/10.1177/095632020001100107---------- VANCOUVER ----------
Wachsman, M.B., López, E.M.F., Ramirez, J.A., Galagovsky, L.R., Coto, C.E. Antiviral effect of brassinosteroids against herpes virus and arenaviruses. Antiviral Chem. Chemother. 2000;11(1):71-77.
http://dx.doi.org/10.1177/095632020001100107