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Abstract:

Hippocampal neuropathology is a recognised feature of the brain in spontaneously hypertensive rats (SHR), but similar studies are lacking in another model of hypertension, the mineralocorticoid-salt-treated rat. The present study aimed to compare changes in hippocampal parameters in 16-week-old male SHR (blood pressure approximately 190 mmHg) and their normotensive Wistar-Kyoto controls, with those of male Sprague-Dawley rats receiving (i) 10 mg deoxycorticosterone acetate (DOCA) every other day during 3 weeks and drinking 1% NaCl solution (blood pressure approximately 160 mmHg) and normotensive controls treated with (ii) DOCA and drinking water, (iii) drinking water only or (iv) 1% NaCl only. In these experimental groups, we determined: (i) cell proliferation in the dentate gyrus (DG) using the 5-bromo-2′-deoxyuridine-labelling technique; (ii) the number of glial fibrillary acidic protein (GFAP) positive astrocytes under the CA1, CA3 and DG; (iii) the number of apolipoprotein E (ApoE) positive astrocytes as a marker of potential neuronal damage; and (iv) the number of neurones in the hilus of the DG, taken as representative of neuronal density in other hippocampal subfields. Changes were remarkably similar in both models, indicating a decreased cell proliferation in DG, an increased number of astrocytes immunopositive for GFAP and ApoE and a reduced number of hilar neurones. Although hypertension may be a leading factor for these abnormalities, endocrine mechanisms may be involved, because hypothalamic-pituitary function, mineralocorticoid receptors and sensitivity to mineralocorticoid treatment are stimulated in SHR, whereas high exogenous mineralocorticoid levels circulate in DOCA-treated rats. Thus, in addition to the deleterious effects of hypertension, endocrine factors may contribute to the abnormalities of hippocampus in SHR and DOCA-treated rats. © 2006 The Authors. Journal compilation © 2006 Blackwell Publishing Ltd.

Registro:

Documento: Artículo
Título:Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats
Autor:Pietranera, L.; Saravia, F.; Gonzalez Deniselle, M.C.; Roig, P.; Lima, A.; De Nicola, A.F.
Filiación:Laboratory of Neuroendocrine Biochemistry, Instituto de Biología y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Department of Biochemistry, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina
Instituto Universitario de Ciencias de la Salud, Fundación Barceló, Buenos Aires, Argentina
Palabras clave:Astrocytes; Dentate gyrus; DOCA-salt hypertension; Hippocampus; Mineralocorticoids; Neurogenesis; SHR; apolipoprotein E; biological marker; broxuridine; deoxycorticosterone acetate; glial fibrillary acidic protein; mineralocorticoid receptor; sodium chloride; analysis of variance; animal experiment; animal model; animal tissue; article; astrocyte; brain damage; cell count; cell density; cell proliferation; controlled study; dentate gyrus; gliosis; hippocampus; hormone blood level; hypertension; hypothalamus hypophysis system; immunocytochemistry; male; morphometrics; nerve cell; nerve cell lesion; nonhuman; priority journal; protein expression; rat; rat strain; sensitivity analysis; spontaneously hypertensive rat; strain difference; Animals; Apolipoproteins E; Astrocytes; Cell Division; Dentate Gyrus; Desoxycorticosterone; Glial Fibrillary Acidic Protein; Gliosis; Hypertension; Male; Neurons; Rats; Rats, Inbred SHR; Rats, Inbred WKY; Rats, Sprague-Dawley; Sodium Chloride; Species Specificity
Año:2006
Volumen:18
Número:6
Página de inicio:466
Página de fin:474
DOI: http://dx.doi.org/10.1111/j.1365-2826.2006.01436.x
Título revista:Journal of Neuroendocrinology
Título revista abreviado:J. Neuroendocrinol.
ISSN:09538194
CODEN:JOUNE
CAS:broxuridine, 59-14-3; deoxycorticosterone acetate, 56-47-3; sodium chloride, 7647-14-5; Apolipoproteins E; Desoxycorticosterone, 64-85-7; Glial Fibrillary Acidic Protein; Sodium Chloride, 7647-14-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09538194_v18_n6_p466_Pietranera

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Citas:

---------- APA ----------
Pietranera, L., Saravia, F., Gonzalez Deniselle, M.C., Roig, P., Lima, A. & De Nicola, A.F. (2006) . Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats. Journal of Neuroendocrinology, 18(6), 466-474.
http://dx.doi.org/10.1111/j.1365-2826.2006.01436.x
---------- CHICAGO ----------
Pietranera, L., Saravia, F., Gonzalez Deniselle, M.C., Roig, P., Lima, A., De Nicola, A.F. "Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats" . Journal of Neuroendocrinology 18, no. 6 (2006) : 466-474.
http://dx.doi.org/10.1111/j.1365-2826.2006.01436.x
---------- MLA ----------
Pietranera, L., Saravia, F., Gonzalez Deniselle, M.C., Roig, P., Lima, A., De Nicola, A.F. "Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats" . Journal of Neuroendocrinology, vol. 18, no. 6, 2006, pp. 466-474.
http://dx.doi.org/10.1111/j.1365-2826.2006.01436.x
---------- VANCOUVER ----------
Pietranera, L., Saravia, F., Gonzalez Deniselle, M.C., Roig, P., Lima, A., De Nicola, A.F. Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats. J. Neuroendocrinol. 2006;18(6):466-474.
http://dx.doi.org/10.1111/j.1365-2826.2006.01436.x