Artículo

Noaín, D.; Avale, M.E.; Wedemeyer, C.; Calvo, D.; Peper, M.; Rubinstein, M. "Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice" (2006) European Journal of Neuroscience. 24(9):2429-2438
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Abstract:

The dopamine D4 receptor (D4R) has received considerable interest because of its higher affinity for atypical antipsychotics, the extremely polymorphic nature of the human gene and the genetic association with attention deficit and hyperactivity disorder (ADHD). Several efforts have been undertaken to determine the D4R expression pattern in the brain using immunohistochemistry, binding autoradiography and in situ hybridization, but the overall published results present large discrepancies. Here, we have explored an alternative genetic approach by studying bacterial artificial chromosome (BAC) transgenic mice that express enhanced green fluorescent protein (EGFP) under the transcriptional control of the mouse dopamine D4 receptor gene (Drd4). Immunohistochemical analysis performed in brain sections of Drd4-EGFP transgenic mice using an anti-EGFP polyclonal antibody showed that transgenic expression was predominant in deep layer neurons of the prefrontal cortex, particularly in the orbital, prelimbic, cingulate and rostral agranular portions. In addition, discrete groups of Drd4-EGFP labelled neurons were observed in the anterior olfactory nucleus, ventral pallidum, and lateral parabrachial nucleus. EGFP was not detected in the striatum, hippocampus or midbrain as described using other techniques. Given the fine specificity of EGFP expression in BAC transgenic mice and the high sensitivity of the EGFP antibody used in this study, our results indicate that Drd4 expression in the adult mouse brain is limited to a more restricted number of areas than previously reported. Its leading expression in the prefrontal cortex supports the importance of the D4R in complex behaviours depending on cortical dopamine (DA) transmission and its possible role in the etiopathophysiology of ADHD. © The Authors (2006).

Registro:

Documento: Artículo
Título:Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice
Autor:Noaín, D.; Avale, M.E.; Wedemeyer, C.; Calvo, D.; Peper, M.; Rubinstein, M.
Filiación:Instituto de Investigaciones en Ingeniería Genética Y Biología Molecular, Consejo Nacional de Investigaciones Científicas Y Técnicas, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular Y Celular, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Argentina
Centro de Estudios Científicos, Valdivia, Chile
Palabras clave:Anterior olfactory nucleus; Green fluorescent protein; Lateral parabrachial nucleus; Prefrontal cortex; Ventral pallidum; dopamine 4 receptor; enhanced green fluorescent protein; animal cell; animal experiment; animal tissue; article; bacterial artificial chromosome; brain nerve cell; cell labeling; cingulate gyrus; corpus striatum; dopaminergic transmission; female; gene expression profiling; gene identification; globus pallidus; hippocampus; immunohistochemistry; limbic cortex; male; mesencephalon; mouse; nonhuman; olfactory nucleus; orbital cortex; parabrachial nucleus; prefrontal cortex; priority journal; receptor gene; transgenic mouse; Animals; Brain; Chromosomes, Artificial, Bacterial; Embryo; Green Fluorescent Proteins; Immunohistochemistry; Male; Mice; Mice, Transgenic; Microscopy, Confocal; Neurons; Patch-Clamp Techniques; Receptors, Dopamine D4
Año:2006
Volumen:24
Número:9
Página de inicio:2429
Página de fin:2438
DOI: http://dx.doi.org/10.1111/j.1460-9568.2006.05148.x
Título revista:European Journal of Neuroscience
Título revista abreviado:Eur. J. Neurosci.
ISSN:0953816X
CODEN:EJONE
CAS:dopamine 4 receptor, 137750-34-6; Green Fluorescent Proteins, 147336-22-9; Receptors, Dopamine D4, 137750-34-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0953816X_v24_n9_p2429_Noain

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Citas:

---------- APA ----------
Noaín, D., Avale, M.E., Wedemeyer, C., Calvo, D., Peper, M. & Rubinstein, M. (2006) . Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice. European Journal of Neuroscience, 24(9), 2429-2438.
http://dx.doi.org/10.1111/j.1460-9568.2006.05148.x
---------- CHICAGO ----------
Noaín, D., Avale, M.E., Wedemeyer, C., Calvo, D., Peper, M., Rubinstein, M. "Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice" . European Journal of Neuroscience 24, no. 9 (2006) : 2429-2438.
http://dx.doi.org/10.1111/j.1460-9568.2006.05148.x
---------- MLA ----------
Noaín, D., Avale, M.E., Wedemeyer, C., Calvo, D., Peper, M., Rubinstein, M. "Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice" . European Journal of Neuroscience, vol. 24, no. 9, 2006, pp. 2429-2438.
http://dx.doi.org/10.1111/j.1460-9568.2006.05148.x
---------- VANCOUVER ----------
Noaín, D., Avale, M.E., Wedemeyer, C., Calvo, D., Peper, M., Rubinstein, M. Identification of brain neurons expressing the dopamine D4 receptor gene using BAC transgenic mice. Eur. J. Neurosci. 2006;24(9):2429-2438.
http://dx.doi.org/10.1111/j.1460-9568.2006.05148.x