Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

N- and P/Q-type voltage dependent calcium channels (VDCCs) mediate transmitter release at neonatal rat neuromuscular junction (NMJ). Thus the neonatal NMJ allows an examination of the coupling of different subtypes of VDCCs to the release process at a single synapse. We studied calcium dependence of transmitter release mediated by each channel by blocking with ω-conotoxin GVIA the N-type channel or with ω-agatoxin IVA the P/Q-type channel while changing the extracellular calcium concentration ([Ca2+]o). Transmitter release mediated by P/Q-type VDCCs showed steeper calcium dependence than N-type mediated release (average slope 3.6 ± 0.09 vs. 2.6 ± 0.03, respectively). Loading the nerve terminals with 10 μM BAPTA-AM in the extracellular solution reduced transmitter release and occluded the blocking effect of ω-conotoxin GVIA (blockade -2 ± 9%) without affecting the action of ω-agatoxin IVA (blockade 85 ± 4%). Both VDCC blockers were able to reduce the amount of facilitation produced by double-pulse stimulation. In these conditions facilitation was restored by increasing [Ca2+]o). The facilitation index (fi) was also reduced by loading nerve terminals with 10 μM BAPTA-AM (fi = 1.2 ± 0.1). The control fi was 2.5 ± 0.1. These results show that P/Q-type VDCCs were more efficiently coupled to neurotransmitter release than were N-type VDCCs at the neonatal neuromuscular junction. This difference could be accounted for by a differential location of these channels at the release site. In addition, our results indicate that space-time overlapping of calcium domains was required for facilitation.

Registro:

Documento: Artículo
Título:Differential Ca2+-dependence of transmitter release mediated by P/Q- and N-type calcium channels at neonatal rat neuromuscular junctions
Autor:Rosato-Siri, M.D.; Piriz, J.; Giugovaz Tropper, B.A.; Uchitel, O.D.
Filiación:Departamento de Biología, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II 2do piso, Buenos Aires (1428), Argentina
Biophysics Sector SISSA/ISAS, Via Beirut 2-4, 34014 Trieste, Italy
Palabras clave:Actives sites; BAPTA-AM; EGTA-AM; Paired-pulse facilitation; calcium channel N type; calcium ion; omega agatoxin; omega conotoxin; 1,2 bis(2 aminophenoxy)ethane N,N,N',N' tetraacetic acid acetoxymethyl ester; 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; agents interacting with transmitter, hormone or drug receptors; calcium; calcium channel blocking agent; calcium channel N type; calcium channel P type; chelating agent; drug derivative; edetic acid; egtazic acid; omega agatoxin IVA; omega conotoxin GVIA; animal tissue; article; concentration (parameters); controlled study; extracellular space; facilitation; nerve ending; nerve stimulation; neuromuscular synapse; neurotransmitter release; nonhuman; priority journal; rat; time; aging; animal; calcium signaling; cell differentiation; comparative study; drug effect; electrostimulation; growth, development and aging; innervation; metabolism; motoneuron; neuromuscular synapse; newborn; physiology; secretion; skeletal muscle; synapse vesicle; synaptic transmission; synaptosome; Wistar rat; Aging; Animals; Animals, Newborn; Calcium; Calcium Channel Blockers; Calcium Channels, N-Type; Calcium Channels, P-Type; Calcium Signaling; Cell Differentiation; Chelating Agents; Edetic Acid; Egtazic Acid; Electric Stimulation; Motor Neurons; Muscle, Skeletal; Neuromuscular Junction; Neurotransmitter Agents; omega-Agatoxin IVA; omega-Conotoxin GVIA; Presynaptic Terminals; Rats; Rats, Wistar; Synaptic Transmission; Synaptic Vesicles
Año:2002
Volumen:15
Número:12
Página de inicio:1874
Página de fin:1880
DOI: http://dx.doi.org/10.1046/j.1460-9568.2002.02015.x
Título revista:European Journal of Neuroscience
Título revista abreviado:Eur. J. Neurosci.
ISSN:0953816X
CODEN:EJONE
CAS:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester, 139890-68-9; Calcium Channel Blockers; Calcium Channels, N-Type; Calcium Channels, P-Type; Calcium, 7440-70-2; Chelating Agents; Edetic Acid, 60-00-4; Egtazic Acid, 67-42-5; Neurotransmitter Agents; omega-Agatoxin IVA; omega-Conotoxin GVIA, 92078-76-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0953816X_v15_n12_p1874_RosatoSiri

Referencias:

  • Artalejo, C.R., Adams, M.E., Fox, A.P., Three types of Ca2+ channel trigger secretion with different efficacies in chromaffin cells (1994) Nature, 367, pp. 72-76
  • Augustine, G.J., Charlton, M.P., Smith, S.J., Calcium action in synaptic transmitter release (1987) Annu. Rev. Neurosci., 10, pp. 633-693
  • Borst, J.G., Sakmann, B., Calcium influx and transmitter release in a fast CNS synapse (1996) Nature, 383, pp. 431-434
  • Bowersox, S.S., Miljanich, G.P., Sugiura, Y., Li, C., Nadasdi, L., Hoffman, B.B., Ramachandran, J., Ko, C.P., Differential blockade of voltage-sensitive calcium channels at the mouse neuromuscular junction by novel omega-conopeptides and omega-agatoxin-IVA (1995) J. Pharmacol. Exp. Ther., 273, pp. 248-256
  • Catterall, W.A., Interactions of presynaptic Ca2+ channels and snare proteins in neurotransmitter release (1999) Ann. NY Acad. Sci., 868, pp. 144-159
  • Cull-Candy, S.G., Lundh, H., Thesleff, S., Effects of botulinum toxin on neuromuscular transmission in the rat (1976) J. Physiol. (Lond.), 260, pp. 177-203
  • Cull-Candy, S.G., Miledi, R., Trautmann, A., Uchitel, O.D., On the release of transmitter at normal, myasthenia gravis and myasthenic syndrome affected human end-plates (1980) J. Physiol. (Lond.), 299, pp. 621-638
  • Dodge, F.A., Rahamimoff, R., Co-operative action a calcium ions in transmitter release at the neuromuscular junction (1967) J. Physiol. (Lond.), 193, pp. 419-432
  • Dudel, J., Inhibition of Ca2+ inflow at nerve terminals of frog muscle blocks facilitation while phasic transmitter release is still considerable (1990) Pflugers. Arch., 415, pp. 566-574
  • Heidelberger, R., Heinemann, C., Neher, E., Matthews, G., Calcium dependence of the rate of exocytosis in a synaptic terminal (1994) Nature, 37, pp. 513-515
  • Iwasaki, S., Momiyama, A., Uchitel, O.D., Takahashi, T., Developmental changes in calcium channel types mediating central synaptic transmission (2000) J. Neurosci., 20, pp. 59-65
  • June, K., Piedras-Rentería, E.S., Smith, S.M., Wheeler, D.B., Lee, S.B., Lee, T.G., Chin, H., Shin, H.-S., Ablation of P/Q-type Ca2+ channel currents, altered synaptic transmission, and progressive ataxia in mice lacking the α1a-subunit (1999) Proc. Natl Acad. Sci. USA, 96, pp. 15245-15250
  • Katz, B., Miledi, R., Further study of the role of calcium in synaptic transmission (1970) J. Physiol. (Lond.), 207, pp. 789-801
  • Katz, E., Protti, D.A., Ferro, P.A., Rosato Siri, M.D., Uchitel, O.D., Effects of Ca2+ channel blocker neurotoxins on transmitter release and presynaptic currents at the mouse neuromuscular junction (1997) Br. J. Pharmacol., 12, pp. 1531-1540
  • Llinas, R., Moreno, H., Local Ca2+ signaling in neurons (1998) Cell Calcium, 24, pp. 359-366
  • Llinas, R., Steinberg, I.Z., Walton, K., Presynaptic calcium currents and their relation to synaptic transmission: Voltage clamp study in squid giant synapse and theoretical model for the calcium gate (1976) Proc. Natl. Acad. Sci. USA, 73, pp. 2913-2922
  • Llinas, R., Sugimori, M., Simon, S.M., Transmission by presynaptic spike-like depolarization in the squid giant synapse (1982) Proc. Natl Acad. Sci. USA, 79, pp. 2415-2419
  • Mintz, I.M., Sabatini, B.L., Regehr, W.G., Calcium control of transmitter release at a cerebellar synapse (1995) Neuron, 15, pp. 675-688
  • Protti, D.A., Uchitel, O.D., Transmitter release and presynaptic Ca2+ currents blocked by the spider toxin omega-Aga-IVA (1993) Neuroreport, 5, pp. 333-336
  • Reid, C.A., Bekkers, J.M., Clements, J.D., N- and P/Q-type Ca2+ channels mediate transmitter release with a similar cooperativity at rat hippocampal autapses (1998) J. Neurosci., 18, pp. 2849-2855
  • Rettig, J., Heinemann, C., Ashery, U., Sheng, Z., Yokoyama, C., Catterall, W., Neher, E., Alteration of Ca2+ dependence of neurotransmitter release by disruption of Ca2+ channel/syntaxin interaction (1997) J. Neurosci., 17, pp. 6647-6656
  • Reuter, H., Diversity and function of presynaptic calcium channels in the brain (1996) Curr. Opin. Neurobiol., 6, pp. 331-337
  • Rosato Siri, M.D., Uchitel, O.D., Calcium channels coupled to neurotransmitter release at neonatal rat neuromuscular junctions (1999) J. Physiol. (Lond.), 514, pp. 533-540
  • Sheng, Z.H., Rettig, J., Takahashi, M., Catterall, W.A., Identification of a syntaxin-binding site on N-type calcium channels (1994) Neuron, 13, pp. 1303-1313
  • Takahashi, T., Forsythe, I.D., Tsujimoto, T., Barnes-Davies, M., Onodera, K., Presynaptic calcium current modulation by a metabotropic glutamate receptor (1996) Science, 274, pp. 594-597
  • Takahashi, T., Momiyama, A., Different types of calcium channels mediate central synaptic transmission (1993) Nature, 366, pp. 156-158
  • Uchitel, O.D., Toxins affecting calcium channels in neurons (1997) Toxicon, 35, pp. 1161-1191
  • Uchitel, O.D., Protti, D.A., Sanchez, V., Cherksey, B.D., Sugimori, M., Llinas, R., P-type voltage-dependent calcium channel mediates presynaptic calcium influx and transmitter release in mammalian synapses (1992) Proc. Natl. Acad. Sci. USA, 89, pp. 3330-3333
  • Urbano, F.J., Piedras-Renteria, E.S., Tsien, R.W., Uchitel, O.D., Short-term facilitation and Ca2+ dependence of transmitter release are altered at the neuromuscular junction of P/Q Ca2+ channel knockout mice (2001) Biophys. J. (Annual Meeting Abstracts), 80, pp. 234a
  • Urbano, F.J., Uchitel, O.D., L-type calcium channels unmasked by cell-permeant Ca2+ buffer at mouse motor nerve terminals (1999) Pflugers Arch., 437, pp. 523-528
  • Wiser, O., Trus, M., Hernandez, A., Renstrom, E., Barg, S., Rorsman, P., Atlas, D., The voltage sensitive Lc-type Ca2+ channel is functionally coupled to the exocytotic machinery (1999) Proc. Natl. Acad. Sci. USA, 96, pp. 248-253
  • Wu, L.G., Saggau, P., Pharmacological identification of two types of presynaptic voltage-dependent calcium channels at CA3-CA1 synapses of the hippocampus (1994) J. Neurosci., 14, pp. 5613-5622
  • Wu, L.G., Westenbroek, R.E., Borst, J.G.G., Catterall, W.A., Sakmann, B., Calcium channel types with distinct presynaptic localization couple differentially to transmitter release in single calyx-type synapses (1999) J. Neurosci., 19, pp. 726-736
  • Zengel, J.E., Lee, D.T., Sosa, M.A., Mosier, D.R., Effects of calcium channel blockers on stimulation-induced changes in transmitter release at the frog neuromuscular junction (1993) Synapse, 15, pp. 251-262
  • Zengel, J.E., Sosa, M.A., Poage, R.E., omega-Conotoxin reduces facilitation of transmitter release at the frog neuromuscular junction (1993) Brain. Res., 611, pp. 25-30
  • Zucker, R.S., Exocytosis: A molecular and physiological perspective (1996) Neuron, 17, pp. 1049-1055

Citas:

---------- APA ----------
Rosato-Siri, M.D., Piriz, J., Giugovaz Tropper, B.A. & Uchitel, O.D. (2002) . Differential Ca2+-dependence of transmitter release mediated by P/Q- and N-type calcium channels at neonatal rat neuromuscular junctions. European Journal of Neuroscience, 15(12), 1874-1880.
http://dx.doi.org/10.1046/j.1460-9568.2002.02015.x
---------- CHICAGO ----------
Rosato-Siri, M.D., Piriz, J., Giugovaz Tropper, B.A., Uchitel, O.D. "Differential Ca2+-dependence of transmitter release mediated by P/Q- and N-type calcium channels at neonatal rat neuromuscular junctions" . European Journal of Neuroscience 15, no. 12 (2002) : 1874-1880.
http://dx.doi.org/10.1046/j.1460-9568.2002.02015.x
---------- MLA ----------
Rosato-Siri, M.D., Piriz, J., Giugovaz Tropper, B.A., Uchitel, O.D. "Differential Ca2+-dependence of transmitter release mediated by P/Q- and N-type calcium channels at neonatal rat neuromuscular junctions" . European Journal of Neuroscience, vol. 15, no. 12, 2002, pp. 1874-1880.
http://dx.doi.org/10.1046/j.1460-9568.2002.02015.x
---------- VANCOUVER ----------
Rosato-Siri, M.D., Piriz, J., Giugovaz Tropper, B.A., Uchitel, O.D. Differential Ca2+-dependence of transmitter release mediated by P/Q- and N-type calcium channels at neonatal rat neuromuscular junctions. Eur. J. Neurosci. 2002;15(12):1874-1880.
http://dx.doi.org/10.1046/j.1460-9568.2002.02015.x