Artículo
Wolski, E.; Maldonado, S.; Daleo, G.; Andreu, A. "Cell wall α-1,3-glucans from a biocontrol isolate of Rhizoctonia: immunocytolocalization and relationship with α-glucanase activity from potato sprouts" (2007) Mycological Research. 111(8):976-984
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Abstract:
The interface between plants and pathogens plays an important role in their interaction. Studies of fungal cell walls are scarce and previous results show the existence of α-1,3-glucans in addition to ß-glucans. In addition, α-1,3-glucans are not present in plant cell walls, and α-glucanase activity in plants has not been described before. In a previous work, we purified and characterized an α-1,3-glucan from a binucleated, non-pathogenic Rhizoctonia isolate, which induces plant defence responses. Therefore, in order to study the architecture of the fungal cell wall, and the accessibility and localization of the α-glucan elicitor, we prepared an antibody against the α-1,3-glucan and analysed its localization by TEM. Immunolocalization showed the presence of the α-1,3-glucan in the intercellular spaces and along the cell walls, mainly on the inner layers. This result, and the presence of the α-1,3-glucan in the liquid culture medium in which binucleated non-pathogenic Rhizoctonia was grown, confirmed that the α-glucan had been secreted. The α-1,3-glucan was also immunocytolocalized on potato sprouts tissue elicited with the glucan; gold particles were observed in vacuoles and close to the plasmalemma. In addition, α-glucanase activity in potato sprouts was detected using cell wall glucans from the pathogenic isolate R. solani AG-3 as substrates; whereas, when cell wall glucans from non-pathogenic isolates were used, no α-glucanase activity was detected. Our results suggest that the presence of α-1,3-glucans could be associated with the formation and integrity of the cell wall and also with plant-fungi interactions. This is the first report to describe α-glucanolytic activity in plants. © 2007 The British Mycological Society.
Registro:
| Documento: | Artículo |
| Título: | Cell wall α-1,3-glucans from a biocontrol isolate of Rhizoctonia: immunocytolocalization and relationship with α-glucanase activity from potato sprouts |
| Autor: | Wolski, E.; Maldonado, S.; Daleo, G.; Andreu, A. |
| Filiación: | Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3250 4to nivel, P.O. Box, 1245 Mar del Plata, Argentina Laboratorio de Anatomía y Embriología Vegetal, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, P.O. Box, 1428 Buenos Aires, Argentina |
| Palabras clave: | Immunoelectron microscopy; Non-pathogenic binucleate; Plant-fungus interactions; Solanum tuberosum; Ultrastructure; 1,3 alpha glucan; alpha-1,3-glucan; glucan; glycosidase; unclassified drug; biological control; cytology; enzyme activity; fungus; plant defense; potato; secretion; article; biological pest control; cell wall; chemistry; enzymology; growth, development and aging; Hyphomycetes; immunoelectron microscopy; isolation and purification; metabolism; potato; transmission electron microscopy; ultrastructure; Cell Wall; Glucans; Glycoside Hydrolases; Microscopy, Electron, Transmission; Microscopy, Immunoelectron; Pest Control, Biological; Rhizoctonia; Solanum tuberosum; Fungi; Hyphomycetes; Rhizoctonia; Solanum tuberosum |
| Año: | 2007 |
| Volumen: | 111 |
| Número: | 8 |
| Página de inicio: | 976 |
| Página de fin: | 984 |
| DOI: | http://dx.doi.org/10.1016/j.mycres.2007.06.012 |
| Título revista: | Mycological Research |
| Título revista abreviado: | Mycol. Res. |
| ISSN: | 09537562 |
| CODEN: | MYCRE |
| CAS: | glucan, 9012-72-0, 9037-91-6; glycosidase, 9032-92-2; Glucans; Glycoside Hydrolases, 3.2.1.-; alpha-1,3-glucan, 9051-95-0 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09537562_v111_n8_p976_Wolski |
Referencias:
- Ait-Lahsen, H., Soler, A., Rey, M., de la Cruz, J., Monte, E., Llobell, A., An antifungal exo-α-1, 3-glucanase (AGNI3.1) from the biocontrol fungus Trichoderma harzianum (2001) Applied Environmental Microbiology, 67, pp. 5833-5839
- Ayers, A., Ebel, J., Valent, B., Albersheim, P., Host-pathogen interaction: X. Fractionation and biological activity of an elicitor isolated from micelial walls of Phytophthora megasperma var. sojae (1976) Plant Physiology, 57, pp. 760-765
- Bartnicki-Garcia, S., Cell wall chemistry, morphogenesis and taxonomy of fungi (1968) Annual Review of Microbiology, 22, pp. 87-108
- Côté, F., Hahn, M.G., Oligasaccharins: structure and signal transduction (1994) Plant Molecular Biology, 26, pp. 1379-1411
- Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., Colorimetric method for determination of sugars and related substances (1956) Analytical Chemistry, 28, pp. 350-356
- Ebel, J., Mithöfer, A., Early events in the elicitation of plant defence (1998) Planta, 206, pp. 335-348
- El Gueddari, N.E., Rauchhaus, U., Moerschbacher, B.M., Deising, H.B., Developmentally regulated conversion of surface-exposed chitin to chitosan in cell walls of plant pathogenic fungi (2002) New Phytologist, 156, pp. 103-112
- Escande, A.R., Echandi, E., Protection of potato from rhizoctonia canker with binucleate Rhizoctonia fungi (1991) Plant Pathology, 40, pp. 197-202
- Esquerré-Tugayé, M.T., Boudart, G., Dumas, B., Cell wall degrading enzymes, inhibitory proteins, and oligosaccharides participate in the molecular dialogue between plants and pathogens (2000) Plant Physiology and Biochemistry, 38, pp. 157-163
- Farkas, V., Biosynthesis of cell walls of fungi (1979) Microbiological Reviews, 43, pp. 117-144
- Hahlbrock, K., Scheel, D., Logemann, E., Nurnberger, T., Parniske, M., Reinold, S., Sacks, W.R., Schmelzer, E., Oligopeptide elicitor-mediated defense gene activation in culture parley cells (1995) Proceedings of the National Academic of Science USA, 92, pp. 4150-4157
- Hahn, M.G., Microbial elicitors and their receptors in plants (1996) Annual Review of Phytopathology, 34, pp. 387-412
- Harris, N., Spence, J., Oparka, K.J., (1994) Plant cell biology: a practical approach, , Harris N., and Oparka K.J. (Eds), Oxford University Press, New York
- Hochstenbach, F., Klis, F.M., van den Ende, H., van Donselaar, E., Peters, P.J., Klausner, R.D., Identification of a putative alpha-glucan synthase essential for cell wall construction and morphogenesis in fission yeast (1998) Proceedings National of the Academic of Sciences USA, 95, pp. 9161-9166
- Hwang, J., Benson, D.M., Expression of induced systemic resistance in poinsettia cuttings against Rhizoctonia stem rot by treatment of stock plants with binucleate Rhizoctonia (2003) Biological Control, 27, pp. 73-80
- Kamoun, S., A catalogue of the effector secretome of plant pathogenic oomycetes (2006) Annual Review of Phytopathology, 44, pp. 41-60
- Katayama, S., Hirata, D., Arellano, M., Pérez, P., Toda, T., Fission yeast α-glucan synthase Mok1 requires the actin cytoskeleton to localize the sites of growth and plays an essential role in cell morphogenesis downstream of protein kinase C function (1999) Journal of Cell Biology, 144, pp. 1173-1186
- Klarzynski, O., Plesse, B., Joubert, J.M., Yvin, J.C., Kopp, M., Kloareg, B., Fritig, B., Linear β-1,3 glucans are elicitors of defense responses in tobacco (2000) Plant Physiology, 124, pp. 1027-1037
- Konomi, M., Fujimoto, K., Toda, T., Osumi, M., Characterization and behaviour of α-glucan synthase in Schizosaccharomyces pombe as revealed by electron microscopy (2003) Yeast, 20, pp. 427-438
- Maxwell, D.P., Lumsden, R., Oxalic, D., Acid production by Sclerotinia sclerotiorum in infected bean and in culture (1970) Phytopathology, 60, pp. 395-1398
- Miyazaki, T., Yamada, M., Ohno, T., Isolation and structure of α-1,3-linked glucan from the hyphal wall of Phytophthora infestans (1974) Chemical and Pharmaceutical Bulletin, 22, pp. 1666-1669
- Nelson, N., A photometric adaptation of the Somogyi method for the determination of glucose (1944) Journal of Biological Chemistry, 153, pp. 375-380
- Peberdy, J.F., (1990) Fungal cell walls: a review. In: Biochemistry of cell walls and membranes in fungi, pp. 5-24. , Kuhn P.J., Trinci A.P.J., Jung M.J., Goosey M.E., and Copping L.G. (Eds), Springer-Verlag, Heidelberg
- Schoffelmeer, E.A.M., Klis, F.M., Sietsma, J.H., Cornelissen, B.J.C., The cell wall of Fusarium oxysporum (1999) Fungal Genetics and Biology, 27, pp. 275-282
- Sneh, B., Ichielevich-Auster, M., Induced Resistance of cucumber seedlings caused by some non-pathogenic Rhizoctonia (np-R) isolates (1998) Phytoparasitica, 26, pp. 27-38
- Somogyi, M., Notes on sugar determination (1952) Journal of Biological Chemistry, 195, pp. 19-23
- Sugawara, T., Sato, M., Takagi, T., Kamasaki, T., Ohno, N., Osumi, M., In situ localization of cell wall α-1,3-glucan in the fission yeast Schizosaccharomyces pombe (2003) Journal of Electron Microscopy, 52, pp. 237-242
- Turner, B.M., Use of alkaline phosphatase-conjugated antibodies for detection of protein antigens on nitrocellulose (1986) Methods in Enzymology, 121, pp. 848-855
- Van Loon, L.C., Rep, M., Pieterse, C.M.J., Significance of inducible defense-related proteins in infected plants (2006) Annual Review of Phytopathology, 44, pp. 135-162
- Wolski, E.A., Daleo, G.R., Andreu, A.B., Induction of pathogenesis-related proteins on potato sprouts by an α-1,3-glucan from cell wall of binucleate non-pathogenic Rhizoctonia sp isolate (2004) Phytopathology, 94, pp. S111
- Wolski, E.A., Lima, C., Agusti, R., Daleo, G.R., Andreu, A.B., de Lederkremer, R.M., An α-glucan elicitor from the cell wall of a biocontrol binucleate Rhizoctonia isolate (2005) Carbohydrate Research, 340, pp. 619-627
- Wolski, E.A., Maldonado, S., Daleo, G.R., Andreu, A.B., A novel α-1,3-glucan elicits plant defense responses in potato and induces protection against Rhizoctonia solani AG-3 and Fusarium solani f. sp. eumartii (2006) Physiological and Molecular Plant Pathology, 69, pp. 93-103
Citas:
---------- APA ----------
Wolski, E., Maldonado, S., Daleo, G. & Andreu, A. (2007) . Cell wall α-1,3-glucans from a biocontrol isolate of Rhizoctonia: immunocytolocalization and relationship with α-glucanase activity from potato sprouts. Mycological Research, 111(8), 976-984.http://dx.doi.org/10.1016/j.mycres.2007.06.012
---------- CHICAGO ----------
Wolski, E., Maldonado, S., Daleo, G., Andreu, A. "Cell wall α-1,3-glucans from a biocontrol isolate of Rhizoctonia: immunocytolocalization and relationship with α-glucanase activity from potato sprouts" . Mycological Research 111, no. 8 (2007) : 976-984.http://dx.doi.org/10.1016/j.mycres.2007.06.012
---------- MLA ----------
Wolski, E., Maldonado, S., Daleo, G., Andreu, A. "Cell wall α-1,3-glucans from a biocontrol isolate of Rhizoctonia: immunocytolocalization and relationship with α-glucanase activity from potato sprouts" . Mycological Research, vol. 111, no. 8, 2007, pp. 976-984.http://dx.doi.org/10.1016/j.mycres.2007.06.012
---------- VANCOUVER ----------
Wolski, E., Maldonado, S., Daleo, G., Andreu, A. Cell wall α-1,3-glucans from a biocontrol isolate of Rhizoctonia: immunocytolocalization and relationship with α-glucanase activity from potato sprouts. Mycol. Res. 2007;111(8):976-984.http://dx.doi.org/10.1016/j.mycres.2007.06.012
