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Abstract:

Forty-two dikaryotic and 42 monokaryotic isolates, and 34 pairings were examined by horizontal polyacrylamide gel electrophoresis (PAGE) for six enzymatic activities, viz. EST, 6PGD, IDH, MDH, SHDH and SOD. 44 bands were analysed. Numerical analysis of the isoenzymatic patterns was undertaken and compared with those from morphological characters. The analysis of six enzymatic systems showed the existence of four monomorphic systems (IDH, MDH, SHDH and SOD). The sterease system (EST) appears to be polymorphic in Polyporus ciliatus and in populations of P. tenuiculus from Argentina, being monomorphic in the remaining species studied. The 6PGD system is polymorphic in P. tucumanensis and monomorphic in the other species. Predominance of monomorphic enzymes and a clear distribution of the electromorphs among the species, indicates that isoenzymatic analysis is a good taxonomic tool within Polyporus. The low intraspecific variability allowed the use of interspecific differences to separate species. Numerical analysis showed a good correlation between morphological and molecular characters. In the isoenzymatic phenogram the similarity index is high only among very close species, showing a stressed separation of species.

Registro:

Documento: Artículo
Título:Polyporus s. str. in southern South America: Isoenzyme analysis
Autor:Borges Da Silveira, R.M.; Saidman, B.O.; Wright, J.E.
Filiación:Departamento de Botânica, Instituto de Biociências, Univ. Federal do Rio Grande do Sul, 90046-900 Porto Alegre, RGS, Brazil
Depto. de Ciencias Biológicas, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:enzyme; fungus; Fungi; Polyporaceae; Polyporus; Polyporus; Polyporus ciliatus; Polyporus ciliatus; Polyporus tenuiculus; Polyporus tenuiculus; Polyporus tucumanensis
Año:2003
Volumen:107
Número:5
Página de inicio:597
Página de fin:608
DOI: http://dx.doi.org/10.1017/S0953756203007469
Título revista:Mycological Research
Título revista abreviado:Mycol. Res.
ISSN:09537562
CODEN:MYCRE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09537562_v107_n5_p597_BorgesDaSilveira

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Citas:

---------- APA ----------
Borges Da Silveira, R.M., Saidman, B.O. & Wright, J.E. (2003) . Polyporus s. str. in southern South America: Isoenzyme analysis. Mycological Research, 107(5), 597-608.
http://dx.doi.org/10.1017/S0953756203007469
---------- CHICAGO ----------
Borges Da Silveira, R.M., Saidman, B.O., Wright, J.E. "Polyporus s. str. in southern South America: Isoenzyme analysis" . Mycological Research 107, no. 5 (2003) : 597-608.
http://dx.doi.org/10.1017/S0953756203007469
---------- MLA ----------
Borges Da Silveira, R.M., Saidman, B.O., Wright, J.E. "Polyporus s. str. in southern South America: Isoenzyme analysis" . Mycological Research, vol. 107, no. 5, 2003, pp. 597-608.
http://dx.doi.org/10.1017/S0953756203007469
---------- VANCOUVER ----------
Borges Da Silveira, R.M., Saidman, B.O., Wright, J.E. Polyporus s. str. in southern South America: Isoenzyme analysis. Mycol. Res. 2003;107(5):597-608.
http://dx.doi.org/10.1017/S0953756203007469