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Abstract:

As part of research to implement methods for the identification of Ganoderma species, this paper describes the use of PCR coupled to restriction digestions, single-strand conformational polymorphism (SSCP) and direct sequencing to assay rDNA polymorphism in South American collections of Ganoderma. The aim of the present study was to determine whether morphologically defined groups could be discriminated by molecular markers and to examine ITS sequence variation. Nineteen isolates of subgenus Ganoderma representing seven morphological taxa, and thirty isolates of subgenus Elfvingia representing three taxa, were studied. ITS I and IT II regions of twenty-one isolates were sequenced and were cladistically analyzed. Seven additional sequences were downloaded from EMBL/Genbank for comparison. In general, agreement between groupings delimited by restriction patterns, SSCP of ITS I and ITS II was evident. Gene trees derived from ITS I, ITS II and from a combined data set, were obtained and compared. Agreement between molecular and morphological data was clear at the subgeneric level; however, at the specific level, this relationship was difficult to visualize.

Registro:

Documento: Artículo
Título:rDNA analyses as an aid to the taxonomy of species of Ganoderma
Autor:Gottlieb, A.M.; Ferrer, E.; Wright, J.E.
Filiación:Departamento de Biología Celular y Genética, Universidad de Alcalá, 28817 Madrid, Spain
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (1428) Buenos Aires, Argentina
Palabras clave:DNA polymorphism; genetic variance; internal transcribed spacer; nucleotide sequence; polymerase chain reaction; ribosomal DNA; single strand conformational polymorphism; South America; fungus; polymerase chain reaction; taxonomy; Ganoderma
Año:2000
Volumen:104
Número:9
Página de inicio:1033
Página de fin:1045
DOI: http://dx.doi.org/10.1017/S095375620000304X
Título revista:Mycological Research
Título revista abreviado:Mycol. Res.
ISSN:09537562
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09537562_v104_n9_p1033_Gottlieb

Referencias:

  • Adaskaveg, J.E., Gilbertson, R.L., Cultural studies and genetics of sexuality of Ganoderma lucidum and G. tsugae in relation with the taxonomy of the G. lucidum complex (1986) Mycologia, 78, pp. 694-705
  • Adaskaveg, J.E., Gilbertson, R.L., Vegetative incompatibility between intraspecific dikaryotic pairings of Ganoderma lucidum and G. tsugae (1987) Mycologia, 79, pp. 603-613
  • Adaskaveg, J.E., Gilbertson, R.L., Cultural studies of 4 North American species in the Ganoderma lucidum complex with the comparison to Ganoderma lucidum and G. tsugae (1989) Mycological Research, 92, pp. 182-191
  • Atkinson, G.F., On the identity of Polyporus applanatus of Europe and North America (1908) Annales Mycologici, 6, pp. 179-191
  • Bassam, B.J., Caetano-Anolles, G., Gresshoff, P.M., Fast and sensitive silver staining of DNA in polyacrylamide gels (1991) Analytical Biochemistry, 196, pp. 81-84
  • Bazzalo, M.E., Wright, J.E., Survey of the Argentine species of the Ganoderma lucidum complex (1982) Mycotaxon, 16, pp. 295-325
  • Berbee, M., Taylor, J.W., Dating the evolutionary radiation of the true fungi (1993) Canadian Journal of Botany, 71, pp. 1114-1127
  • Buchanan, P.K., Wilkie, P., Taxonomy of New Zealand Ganoderma - Two non-laccate species (1995) Proceedings of Contributed Symposium 59A, B 5th International Mycological Congress, pp. 7-17. , (P. K. Buchanan, R. S. Hseu and J. M. Moncalvo, eds). Academia Sinica, Taipei
  • Bunyard, B.A., Nicholson, M.S., Royse, D.J., Phylogeny of the genus Agaricus inferred from restriction analysis of enzymatically amplified ribosomal DNA (1996) Fungal Genetics and Biology, 20, pp. 243-253
  • Cantrell, S.A., Hanlin, R.T., Phylogenetic relationships in the family Hyaloscyphaceae inferred from sequences of ITS regions, 5.8S ribosomal DNA and morphological characters (1997) Mycologia, 89, pp. 745-755
  • Choi, S.H., Kim, B.K., Kim, H.W., Kwak, J.H., Choi, E.C., Kim, Y.C., Yoo, Y.B., Park, Y.H., Studies on protoplast formation and regeneration of Ganoderma lucidum (1987) Archives of Pharmacological Research, 10, pp. 158-164
  • Corner, E.J.H., Ad Polyporaceae I. Amauroderma and Ganoderma (1983) Beihefte zur Nova Hedwigia, 75, pp. 1-182
  • Curtis, W., (1781) Flora Londinensis, or plates and descriptions of such plants as grow wild in the environs of London. 2nd edn., , London
  • Fouly, H.M., Wilkinson, H.T., Chen, W., Restriction analysis of internal transcribed spacers and the small subunit gene of ribosomal DNA among four Gaeumannomyces species (1997) Mycologia, 89, pp. 590-597
  • Gasser, R.B., Monti, J.R., Zhu, X., Chilton, N.B., Hung, G.C., Guldberg, P., Polymerase chain reaction-linked single-strand conformation polymorphism of ribosomal DNA to fingerprint parasites (1997) Electrophoresis, 18, pp. 1564-1566
  • Gilbertson, R.L., Ryvarden, L., (1986) North American Polypores, 1. , Fungiflora, Oslo
  • Gottlieb, A.M., Saidman, B.O., Wright, J.E., Isoenzymes of Ganoderma species from southern South America (1998) Mycological Research, 102, pp. 415-426
  • Gottlieb, A.M., Wright, J.E., Taxonomy of the genus Ganoderma from southern South America: Subgenus Ganoderma (1999) Mycological Research, 103, pp. 661-673
  • Gottlieb, A.M., Wright, J.E., Taxonomy of the genus Ganoderma from southern South America: Subgenus Elfvingia (1999) Mycological Research, 103, pp. 1289-1298
  • Hayashi, K., PCR-SSCP: A simple and sensitive method for detection of mutations in the genomic DNA (1991) PCR Methods and Applications, 1, pp. 34-38
  • Henrion, B., Chevalier, G., Martin, F., Typing truffle species by PCR amplification of the ribosomal DNA spacers (1994) Mycological Research, 98, pp. 37-43
  • Hibbett, D.S., Vilgalys, R., Evolutionary relationships of Lentinus to the Polyporaceae: Evidence from restriction analysis of enzymatically amplified ribosomal DNA (1991) Mycologia, 83, pp. 425-439
  • Hillis, D.M., Dixon, M.T., Ribosomal DNA: Molecular evolution and phylogenetic inference (1991) Quarterly Review of Biology, 66, pp. 411-453
  • Hiss, R.H., Norris, C.H., Dietrich, R.F., Whitcomb, D.F., Bosio, C.F., Kambhampati, S., Piesmman, J., Black, C., Molecular taxonomy using single-strand conformation polymorphism (SSCP) analysis of mitochondrial ribosomal DNA genes (1994) Insect Molecular Biology, 3, pp. 171-182
  • Hopple, J.S., Vilgalys, R., Phylogenetic relationships among coprinoid taxa and allies based on data from restriction site mapping of nuclear DNA (1994) Mycologia, 86, pp. 96-107
  • Holst-Jensen, A., Kohn, L.M., Schumacher, T., Nuclear rDNA phylogeny of the Sclerotiniaceae (1997) Mycologia, 89, pp. 885-899
  • Hseu, R.S., (1990) An identification system for cultures of Ganoderma species, , PhD dissertation, National Taiwan University, Taipei. [In Chinese.]
  • Humphrey, C.J., Leus, S., A partial revision of the Ganoderma applanatum group, with particular reference to its oriental variants (1931) Philippine Journal of Science, 45, pp. 483-589
  • Liu, Y.J., Rogers, S.O., Ammirati, J.F., Phylogenetic relationships in Dermocybe and related Cortinarius taxa based on nuclear ribosomal DNA internal transcribed spacers (1997) Canadian Journal of Botany, 75, pp. 519-532
  • Lutzoni, F., Vilgalys, R., Integration of morphological and molecular data sets in estimating fungal phylogenies (1995) Canadian Journal of Botany, 73, pp. S649-S659
  • Karsten, P.A., Enumeratio boletinarum et polyporarum fennicarum systemate novo dispositorum (1881) Revue de Mycologie, 3, pp. 16-19
  • Kiss, L., Genetic diversity in Ampelomyces isolates, hyperparasites of powdery mildew fungi, inferred from RFLP analysis of the rDNA ITS region (1997) Mycological Research, 101, pp. 1073-1080
  • Ko, K.S., Hong, S.G., Jung, H.S., Phylogenetic analysis of Trichaptum based on nuclear 18S, 5.8S and ITS ribosomal DNA sequences (1997) Mycologia, 89, pp. 727-734
  • Kumeda, Y., Asao, T., Single-strand conformation polymorphism analysis of PCR-amplified ribosomal DNA internal transcribed spacers to differentiate species of Aspergillus section Flavi (1996) Applied and Environmental Microbiology, 62, pp. 2947-2952
  • Moncalvo, J.M., Wang, H.H., Hseu, R.S., Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences (1995) Mycologia, 87, pp. 223-238
  • Moncalvo, J.M., Wang, H.F., Hseu, R.S., Gene phylogeny of the Ganoderma lucidum complex. Comparison with traditional taxonomic characters (1995) Mycological Research, 99, pp. 1489-1499
  • Moncalvo, J.M., Wang, H.F., Wang, H.H., Hseu, R.S., The use of rDNA nucleotide sequence data for species identification and phylogeny in the Ganodermataceae (1995) Proceedings of Contributed Symposium 59a, B 5th International Mycological Congress, pp. 31-44. , (P. K. Buchanan, R. S. Hseu and J. M. Moncalvo, eds). Academia Sinica, Taipei
  • Moricca, S., Ragazzi, A., Use of RFLP and SSCP analysis to differentiate the pine rusts Cronartium flaccidum and Peridermium pini (1988) Mycological Research, 102, pp. 666-670
  • Nicholson, M.S., Bunyard, B.A., Royse, D.J., Phylogeny of the genus Lentinula based on ribosomal DNA restriction fragment length polymorphism analysis (1997) Mycologia, 89, pp. 400-407
  • Patouillard, N., Le genre Ganoderma (1899) Bulletin de la Societe Mycologique de France, 5, pp. 64-80
  • Pegler, D.N., Young, T.W.K., Basidiospore form in the British species of Ganoderma Karst (1973) Kew Bulletin, 28, pp. 351-364
  • Rolf, J.F., (1993) NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System. Version 1.8, , Exeter Software. Applied Biostatistics, NY
  • Ryvarden, L., (1991) Genera of Polypores. Nomenclature and taxonomy, , [Synopsis Fungorum No. 5.] Fungiflora, Oslo
  • Sarkar, G., Yoon, H.S., Sommer, S.S., Screening for mutations by RNA single-strand conformation polymorphism (rSSCP): Comparison with DNA-SSCP (1992) Nucleic Acids Research, 20, pp. 871-878
  • Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning. A laboratory manual. Book 3. 2nd edn., , Cold Spring Harbor Laboratory Press, Cold Spring Harbor
  • Scherriff, C., Whelan, M.J., Arnold, G.M., Lafay, J.F., Brygoo, Y., Bailey, J.A., Ribosomal DNA sequence analysis reveals new species groupings in the genus Colletrotrichum (1994) Experimental Mycology, 18, pp. 121-138
  • Steyaert, R.L., Considerations generales sur le genre Ganoderma et plus specialement sur les especes europeenes (1967) Bulletin de la Societe Royale de Botanique de Belgique, 100, p. 189
  • Swofford, D.L., (1993) PAUP: Phylogenetic analysis using parsimony. Version 3.1, , Illinois Natural History Survey, Champaign
  • Taylor, J.W., Bruns, T.D., White, T.J., Can the amount of molecular divergence define species and genera: Comparison between Sordariales and Agaricales (1990), p. 343. , Abstract. Fourth International Mycological Congress, Regensburg, Germany. Fourth International Mycological Congress, Regensburg; Thomas, A.W., Morgan, R., Sweeney, M., Rees, A., Alcolado, J., The detection of mitochondrial DNA mutations using single-strand conformation polymorphism (SSCP) analysis and heteroduplex analysis (1994) Human Genetics, 94, pp. 621-623
  • Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice (1994) Nucleic Acids Research, 22, pp. 4673-4680
  • Vilgalys, R., Hester, M., Rapid genetic identification and mapping of enzimatically amplified ribosomal DNA from several Cryptococcus species (1990) Journal of Bacteriology, 172, pp. 4238-4246
  • Weiland, J.J., Rapid procedure for the extraction of DNA from fungal spores and mycelia (1997) Fungal Genetics Newsletter, 44, pp. 60-63
  • Zhao, J.D., The Ganodermataceae in China (1989) Bibliotheca Mycologica, 132, pp. 1-176

Citas:

---------- APA ----------
Gottlieb, A.M., Ferrer, E. & Wright, J.E. (2000) . rDNA analyses as an aid to the taxonomy of species of Ganoderma. Mycological Research, 104(9), 1033-1045.
http://dx.doi.org/10.1017/S095375620000304X
---------- CHICAGO ----------
Gottlieb, A.M., Ferrer, E., Wright, J.E. "rDNA analyses as an aid to the taxonomy of species of Ganoderma" . Mycological Research 104, no. 9 (2000) : 1033-1045.
http://dx.doi.org/10.1017/S095375620000304X
---------- MLA ----------
Gottlieb, A.M., Ferrer, E., Wright, J.E. "rDNA analyses as an aid to the taxonomy of species of Ganoderma" . Mycological Research, vol. 104, no. 9, 2000, pp. 1033-1045.
http://dx.doi.org/10.1017/S095375620000304X
---------- VANCOUVER ----------
Gottlieb, A.M., Ferrer, E., Wright, J.E. rDNA analyses as an aid to the taxonomy of species of Ganoderma. Mycol. Res. 2000;104(9):1033-1045.
http://dx.doi.org/10.1017/S095375620000304X