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Abstract:

The mechanisms of the teratogenic effects of maternal alcohol consumption remain unclear. The aim of the present work was to study the organogenic PGE2 levels and the modulation of PGE2 levels by NO after periconceptional alcohol ingestion. Female mice were intoxicated with a 10% ethanol in drinking water before pregnancy and up to day 10 of gestation. The PGE2 released from organogenic embryos was measured by radio immunoassay following incubation with or without the addition of either a NO donor or a NO synthase (NOS) inhibitor. In the ethanol-treated females, we found increased percentages of retarded embryos, associated with a significantly elevated resorption rate (p < 0.05), very high quantities of morphologically abnormal E.10 embryos (p < 0.001) and significantly increased PGE2 release, as compared to the embryo parameters of control females. While in the control-derived E.10 embryos the NO donor produced significantly increased PGE2 release, in the ethanol-derived embryos decreased quantities of PGE2 were observed. L-NMMA inhibited PGE2 release in both control and ethanol-derived embryos at different concentrations, whereas it decreased PGE2 content in controls but not in ethanol-derived embryos. The periconceptional alcohol ingestion produced excessive PGE2 release, decreased PGE2 content and disruption of the regulatory NO-PGE2 pathways. These PGs alterations may be related to delayed organogenesis and abnormal neural tube development after chronic periconceptional consumption of alcohol. © 2007.

Registro:

Documento: Artículo
Título:Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide
Autor:Cebral, E.; Faletti, A.; Jawerbaum, A.; Paz, D.
Filiación:Laboratorio de Biología del Desarrollo, Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), DBBE, 4to. Piso, Pab.II, Buenos Aires, (CP 1428EHA), Argentina
Centro de Estudios Farmacológicos y Botánicos (CEFYBO-CONICET), Paraguay 2157, Buenos Aires, Argentina
Palabras clave:alcohol; drinking water; n(g) methylarginine; nitric oxide; nitric oxide donor; nitric oxide synthase inhibitor; prostaglandin E; alcohol consumption; alcohol intoxication; animal experiment; animal tissue; article; controlled study; embryo; embryo development; embryo resorption; embryopathy; female; gestation period; mouse; neural tube; neural tube defect; nonhuman; organogenesis; pregnancy; priority journal; prostaglandin release; radioimmunoassay; Alcohol Drinking; Animals; Body Weight; Central Nervous System Depressants; Embryo; Embryo Implantation; Embryonic Development; Enzyme Inhibitors; Ethanol; Female; Mice; Nitric Oxide Donors; Nitric Oxide Synthase; omega-N-Methylarginine; Organogenesis; Pregnancy; Prostaglandins E; Radioimmunoassay; Mus
Año:2007
Volumen:76
Número:3
Página de inicio:141
Página de fin:151
DOI: http://dx.doi.org/10.1016/j.plefa.2006.12.001
Título revista:Prostaglandins Leukotrienes and Essential Fatty Acids
Título revista abreviado:Prostaglandins Leukotrienes Essent. Fatty Acids
ISSN:09523278
CODEN:PLEAE
CAS:alcohol, 64-17-5; n(g) methylarginine, 156706-47-7, 17035-90-4; nitric oxide, 10102-43-9; prostaglandin E, 11042-70-9; Central Nervous System Depressants; Enzyme Inhibitors; Ethanol, 64-17-5; Nitric Oxide Donors; Nitric Oxide Synthase, EC 1.14.13.39; omega-N-Methylarginine, 17035-90-4; Prostaglandins E
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09523278_v76_n3_p141_Cebral

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Citas:

---------- APA ----------
Cebral, E., Faletti, A., Jawerbaum, A. & Paz, D. (2007) . Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide. Prostaglandins Leukotrienes and Essential Fatty Acids, 76(3), 141-151.
http://dx.doi.org/10.1016/j.plefa.2006.12.001
---------- CHICAGO ----------
Cebral, E., Faletti, A., Jawerbaum, A., Paz, D. "Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide" . Prostaglandins Leukotrienes and Essential Fatty Acids 76, no. 3 (2007) : 141-151.
http://dx.doi.org/10.1016/j.plefa.2006.12.001
---------- MLA ----------
Cebral, E., Faletti, A., Jawerbaum, A., Paz, D. "Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide" . Prostaglandins Leukotrienes and Essential Fatty Acids, vol. 76, no. 3, 2007, pp. 141-151.
http://dx.doi.org/10.1016/j.plefa.2006.12.001
---------- VANCOUVER ----------
Cebral, E., Faletti, A., Jawerbaum, A., Paz, D. Periconceptional alcohol consumption-induced changes in embryonic prostaglandin E levels in mouse organogenesis: Modulation by nitric oxide. Prostaglandins Leukotrienes Essent. Fatty Acids. 2007;76(3):141-151.
http://dx.doi.org/10.1016/j.plefa.2006.12.001