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Abstract:

The effects of leukotriene C4 (LTC4), nordihydroguaiaretic acid (NDGA) and FPL-55712, on the metabolism of labelled glucose (U14C-glucose) in uteri isolated from spayed rats and from spayed-estrogenized rats, incubated in the presence and in the absence of indomethacin, were explored. Indomethacin (10-6M), enhanced significantly 14CO2 formation from labelled glucose, both in uteri from ovariectomized rats and in uteri from ovariectomized-estrogenized animals. In uteri from spayed not-estrogenized rats, exposed 'in vitro' to indomethacin, NDGA (10-5M), an inhibitor of the 5-lipoxygenase, as well as FPL-55712 (10-5M), a LT antagonist, reduced significantly the enhanced metabolism of glucose evoked by indomethacin, an inhibitor of the cyclo- oxygenase. On the other hand, LTC4 (10-7M) augmented the metabolism of labelled glucose, reaching values even greater than those induced by indomethacin. In the spayed-estrogenized group LTC4 (10-10 - 10-7M) enhanced the formation of labelled CO2 from labelled glucose as much as indomethacin (10-6M) did, whereas neither NDGA nor FPL-55712 were effective. In addition, in uteri from ovariectomized-estrogenized rats, incubated with indomethacin, NDGA and FPL-55712, decreased the augmenting action of indomethacin on glucose metabolism, whereas LTC4 (10-10 - 10-7M) evoked a complete reversal of the inhibitory influence of NDGA on the formation of 14CO2. The force-going results suggest that tissue 5-lipoxygenase products, particularly LTC4, are involved in the metabolism of labelled glucose by rat uteri, mainly when the cyclo-oxygenase pathway is inhibited by indomethacin and the tissue is deprived of estradiol. Moreover, under estrogenic dominance, exogenous LTC4 exhibits a peculiar influence manifested by an enhancement of the uterine metabolism of U14C-glucose. © 1989.

Registro:

Documento: Artículo
Título:The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin
Autor:Gonzalez, E.T.; Gimeno, A.L.; Gimeno, M.A.F.
Filiación:Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET), Universidad Nacional de Buenos Aires, Argentina
Departamento de Farmacologia y Toxicologia, Facultad de Medicine, Universidad Nacional de Buenos Aires, Argentina
Palabras clave:7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid; carbon dioxide; chromone derivative; estradiol; glucose; indometacin; nordihydroguaiaretic acid; slow reacting substance; 7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid; estradiol; glucose; indometacin; leukotriene c4; nordihydroguaiaretic acid; radioisotope; animal; article; enzymology; female; in vitro study; metabolism; ovariectomy; rat; rat strain; uterus; animal cell; metabolism; nonhuman; priority journal; subcutaneous drug administration; Animal; Carbon Dioxide; Chromones; Estradiol; Female; Glucose; In Vitro; Indomethacin; Nordihydroguaiaretic Acid; Ovariectomy; Rats; Rats, Inbred Strains; SRS-A; Support, Non-U.S. Gov't; Uterus
Año:1989
Volumen:36
Número:2
Página de inicio:113
Página de fin:117
DOI: http://dx.doi.org/10.1016/0952-3278(89)90028-8
Título revista:Prostaglandins, Leukotrienes and Essential Fatty Acids
Título revista abreviado:Prostaglandins Leukotrienes Essent. Fatty Acids
ISSN:09523278
CODEN:PLEAE
CAS:7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid, 40786-08-1; carbon dioxide, 124-38-9, 58561-67-4; estradiol, 50-28-2; glucose, 50-99-7, 84778-64-3; indometacin, 53-86-1, 74252-25-8, 7681-54-1; nordihydroguaiaretic acid, 500-38-9; leukotriene C4, 72025-60-6; Carbon Dioxide, 124-38-9; Chromones; Estradiol, 50-28-2; FPL 55712, 40786-08-1; Glucose, 50-99-7; Indomethacin, 53-86-1; Nordihydroguaiaretic Acid, 500-38-9; SRS-A
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09523278_v36_n2_p113_Gonzalez

Referencias:

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  • Gonzalez, E. T., Gimeno, M. A. F. and Gimeno, A. L. Prostaglandin E2 alters the metabolism of labelled glucose in uteri isolated from ovariectomized rats. Effects of 17-Beta-estradiol and indomethacin. Prost. Leuk. Ess. Fatty Acids (accepted for publication); Mitchell, Grzyboski, Dehar, Hunter, Arachidonic acid metabolism by lipoxygenase pathways in uterine and intrauterine tissues of pregnant sheep (1987) Prost. Leuk. and Med., 27, pp. 197-207
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  • Cheng, Lang, Againdra, Townley, Tissue distribution and functional correlation of 3H-leukotriene C4 and 3H-leukotriene D4 binding sites in guinea pig uterus and lung preparations (1985) J. Pharmacol. Exp. Ther., 232, pp. 80-85
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Citas:

---------- APA ----------
Gonzalez, E.T., Gimeno, A.L. & Gimeno, M.A.F. (1989) . The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin. Prostaglandins, Leukotrienes and Essential Fatty Acids, 36(2), 113-117.
http://dx.doi.org/10.1016/0952-3278(89)90028-8
---------- CHICAGO ----------
Gonzalez, E.T., Gimeno, A.L., Gimeno, M.A.F. "The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin" . Prostaglandins, Leukotrienes and Essential Fatty Acids 36, no. 2 (1989) : 113-117.
http://dx.doi.org/10.1016/0952-3278(89)90028-8
---------- MLA ----------
Gonzalez, E.T., Gimeno, A.L., Gimeno, M.A.F. "The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin" . Prostaglandins, Leukotrienes and Essential Fatty Acids, vol. 36, no. 2, 1989, pp. 113-117.
http://dx.doi.org/10.1016/0952-3278(89)90028-8
---------- VANCOUVER ----------
Gonzalez, E.T., Gimeno, A.L., Gimeno, M.A.F. The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin. Prostaglandins Leukotrienes Essent. Fatty Acids. 1989;36(2):113-117.
http://dx.doi.org/10.1016/0952-3278(89)90028-8