Abstract:
The effects of leukotriene C4 (LTC4), nordihydroguaiaretic acid (NDGA) and FPL-55712, on the metabolism of labelled glucose (U14C-glucose) in uteri isolated from spayed rats and from spayed-estrogenized rats, incubated in the presence and in the absence of indomethacin, were explored. Indomethacin (10-6M), enhanced significantly 14CO2 formation from labelled glucose, both in uteri from ovariectomized rats and in uteri from ovariectomized-estrogenized animals. In uteri from spayed not-estrogenized rats, exposed 'in vitro' to indomethacin, NDGA (10-5M), an inhibitor of the 5-lipoxygenase, as well as FPL-55712 (10-5M), a LT antagonist, reduced significantly the enhanced metabolism of glucose evoked by indomethacin, an inhibitor of the cyclo- oxygenase. On the other hand, LTC4 (10-7M) augmented the metabolism of labelled glucose, reaching values even greater than those induced by indomethacin. In the spayed-estrogenized group LTC4 (10-10 - 10-7M) enhanced the formation of labelled CO2 from labelled glucose as much as indomethacin (10-6M) did, whereas neither NDGA nor FPL-55712 were effective. In addition, in uteri from ovariectomized-estrogenized rats, incubated with indomethacin, NDGA and FPL-55712, decreased the augmenting action of indomethacin on glucose metabolism, whereas LTC4 (10-10 - 10-7M) evoked a complete reversal of the inhibitory influence of NDGA on the formation of 14CO2. The force-going results suggest that tissue 5-lipoxygenase products, particularly LTC4, are involved in the metabolism of labelled glucose by rat uteri, mainly when the cyclo-oxygenase pathway is inhibited by indomethacin and the tissue is deprived of estradiol. Moreover, under estrogenic dominance, exogenous LTC4 exhibits a peculiar influence manifested by an enhancement of the uterine metabolism of U14C-glucose. © 1989.
Registro:
Documento: |
Artículo
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Título: | The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin |
Autor: | Gonzalez, E.T.; Gimeno, A.L.; Gimeno, M.A.F. |
Filiación: | Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET), Universidad Nacional de Buenos Aires, Argentina Departamento de Farmacologia y Toxicologia, Facultad de Medicine, Universidad Nacional de Buenos Aires, Argentina
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Palabras clave: | 7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid; carbon dioxide; chromone derivative; estradiol; glucose; indometacin; nordihydroguaiaretic acid; slow reacting substance; 7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid; estradiol; glucose; indometacin; leukotriene c4; nordihydroguaiaretic acid; radioisotope; animal; article; enzymology; female; in vitro study; metabolism; ovariectomy; rat; rat strain; uterus; animal cell; metabolism; nonhuman; priority journal; subcutaneous drug administration; Animal; Carbon Dioxide; Chromones; Estradiol; Female; Glucose; In Vitro; Indomethacin; Nordihydroguaiaretic Acid; Ovariectomy; Rats; Rats, Inbred Strains; SRS-A; Support, Non-U.S. Gov't; Uterus |
Año: | 1989
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Volumen: | 36
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Número: | 2
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Página de inicio: | 113
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Página de fin: | 117
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DOI: |
http://dx.doi.org/10.1016/0952-3278(89)90028-8 |
Título revista: | Prostaglandins, Leukotrienes and Essential Fatty Acids
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Título revista abreviado: | Prostaglandins Leukotrienes Essent. Fatty Acids
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ISSN: | 09523278
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CODEN: | PLEAE
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CAS: | 7 [3 (4 acetyl 3 hydroxy 2 propylphenoxy) 2 hydroxypropoxy] 4 oxo 8 propyl 4h 1 benzopyran 2 carboxylic acid, 40786-08-1; carbon dioxide, 124-38-9, 58561-67-4; estradiol, 50-28-2; glucose, 50-99-7, 84778-64-3; indometacin, 53-86-1, 74252-25-8, 7681-54-1; nordihydroguaiaretic acid, 500-38-9; leukotriene C4, 72025-60-6; Carbon Dioxide, 124-38-9; Chromones; Estradiol, 50-28-2; FPL 55712, 40786-08-1; Glucose, 50-99-7; Indomethacin, 53-86-1; Nordihydroguaiaretic Acid, 500-38-9; SRS-A
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09523278_v36_n2_p113_Gonzalez |
Referencias:
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- Gonzalez, E. T., Gimeno, M. A. F. and Gimeno, A. L. Prostaglandin E2 alters the metabolism of labelled glucose in uteri isolated from ovariectomized rats. Effects of 17-Beta-estradiol and indomethacin. Prost. Leuk. Ess. Fatty Acids (accepted for publication); Mitchell, Grzyboski, Dehar, Hunter, Arachidonic acid metabolism by lipoxygenase pathways in uterine and intrauterine tissues of pregnant sheep (1987) Prost. Leuk. and Med., 27, pp. 197-207
- Saeed, Mitchell, Lipoxygenase activity in human uterine and intrauterine tissues: new prospects for control of prostacyclin production in preeclampsia. Cli. and Exper (1983) Hyper-Hyper in pregnancy B2, pp. 103-111
- Thaler-Dao, Jouanen, Benmehdi, Crastes de Paulet, Regulation by estradiol of the lipoxygenase pathway in the rat uterus (1985) Prost. Leuk. and Med., 18, pp. 59-64
- Borgeat, Samuelsson, Transformation of arachidonic acid by rabbit polymorphonuclear leukocytes (1979) J. Biol. Chem., 254, pp. 2643-2646
- Malathy, Cheng, Dey, Production of leukotrienes and prostaglandins in the rat uterus during periimplantation period (1986) Prostaglandins, 32, pp. 605-614
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Citas:
---------- APA ----------
Gonzalez, E.T., Gimeno, A.L. & Gimeno, M.A.F.
(1989)
. The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin. Prostaglandins, Leukotrienes and Essential Fatty Acids, 36(2), 113-117.
http://dx.doi.org/10.1016/0952-3278(89)90028-8---------- CHICAGO ----------
Gonzalez, E.T., Gimeno, A.L., Gimeno, M.A.F.
"The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin"
. Prostaglandins, Leukotrienes and Essential Fatty Acids 36, no. 2
(1989) : 113-117.
http://dx.doi.org/10.1016/0952-3278(89)90028-8---------- MLA ----------
Gonzalez, E.T., Gimeno, A.L., Gimeno, M.A.F.
"The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin"
. Prostaglandins, Leukotrienes and Essential Fatty Acids, vol. 36, no. 2, 1989, pp. 113-117.
http://dx.doi.org/10.1016/0952-3278(89)90028-8---------- VANCOUVER ----------
Gonzalez, E.T., Gimeno, A.L., Gimeno, M.A.F. The influences of leukotriene C4 on the metabolism of labelled glucose in uteri isolated from ovariectomized and from ovariectomized-estrogenized rats. Effects of indomethacin. Prostaglandins Leukotrienes Essent. Fatty Acids. 1989;36(2):113-117.
http://dx.doi.org/10.1016/0952-3278(89)90028-8