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Papa Gobbi, R.; De Francesco, N.; Bondar, C.; Muglia, C.; Chirdo, F.; Rumbo, M.; Rocca, A.; Toscano, M.A.; Sambuelli, A.; Rabinovich, G.A.; Docena, G.H. "A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders" (2016) BioFactors. 42(1):93-105
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Abstract:

Inflammatory bowel diseases (IBD) are chronic and relapsing inflammatory conditions of the gastrointestinal tract including Crohn's disease (CD) and ulcerative colitis (UC). Galectins, defined by shared consensus amino acid sequence and affinity for β-galactosides, are critical modulators of the inflammatory response. However, the relevance of the galectin network in the pathogenesis of human IBD has not yet been explored. Here, we analyzed the expression of relevant members of the galectin family in intestinal biopsies, and identified their contribution as novel mucosal markers in IBD. Colonic biopsies were obtained from 59 IBD patients (22 CD and 37 UC), 9 patients with gut rejection after transplantation, 8 adult celiac patients, and 32 non-IBD donors. Galectin mRNA expression was analyzed by RT-PCR and qPCR using specific primers for individual galectins. A linear discriminant analysis (LDA) was used to analyze galectin expression in individual intestinal samples. Expression of common mucosal-associated galectins (Gal-1, -3, -4, -9) is dysregulated in inflamed tissues of IBD patients compared with non-inflamed IBD or control samples. LDA discriminated between different inflammation grades in active IBD and showed that remission IBD samples were clusterized with control samples. Galectin profiling could not distinguish CD and UC. Furthermore, inflamed IBD was discriminated from inflamed tissue of rejected gut in transplanted patients and duodenum of celiac patients, which could not be distinguished from control duodenum samples. The integrative analysis of galectins discriminated IBD from other intestinal inflammatory conditions and could be used as potential mucosal biomarker. © 2016 International Union of Biochemistry and Molecular Biology.

Registro:

Documento: Artículo
Título:A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders
Autor:Papa Gobbi, R.; De Francesco, N.; Bondar, C.; Muglia, C.; Chirdo, F.; Rumbo, M.; Rocca, A.; Toscano, M.A.; Sambuelli, A.; Rabinovich, G.A.; Docena, G.H.
Filiación:Instituto de Estudios Inmunológicos y Fisiopatológicos-(IIFP), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina
Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina
Servicio de Enfermedades Inflamatorias, Hospital de Gastroenterología Dr. Bonorino Udaondo, Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Biomarkers; Crohn disease; Galectins; Inflammatory bowel diseases; Ulcerative colitis; ecalectin; galectin; galectin 3; galectin 4; messenger RNA; 1-nitrohydroxyphenyl-N-benzoylalanine; benzamide derivative; galectin; galectin 3; galectin 4; LGALS9 protein, human; messenger RNA; tyrosine; adult; aged; Article; colon biopsy; controlled study; Crohn disease; discriminant analysis; enteropathy; female; human; human tissue; inflammation; intestine; major clinical study; male; priority journal; protein expression; quantitative analysis; remission; reverse transcription polymerase chain reaction; ulcerative colitis; analogs and derivatives; biopsy; biosynthesis; Crohn disease; gastrointestinal tract; gene expression regulation; genetics; intestine; intestine mucosa; metabolism; pathology; ulcerative colitis; Adult; Benzamides; Biopsy; Colitis, Ulcerative; Crohn Disease; Female; Galectin 3; Galectin 4; Galectins; Gastrointestinal Tract; Gene Expression Regulation; Humans; Inflammation; Intestinal Mucosa; Intestines; Male; RNA, Messenger; Tyrosine
Año:2016
Volumen:42
Número:1
Página de inicio:93
Página de fin:105
DOI: http://dx.doi.org/10.1002/biof.1252
Título revista:BioFactors
Título revista abreviado:BioFactors
ISSN:09516433
CODEN:BIFAE
CAS:galectin 3, 208128-56-7; tyrosine, 16870-43-2, 55520-40-6, 60-18-4; 1-nitrohydroxyphenyl-N-benzoylalanine; Benzamides; Galectin 3; Galectin 4; Galectins; LGALS9 protein, human; RNA, Messenger; Tyrosine
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09516433_v42_n1_p93_PapaGobbi

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Citas:

---------- APA ----------
Papa Gobbi, R., De Francesco, N., Bondar, C., Muglia, C., Chirdo, F., Rumbo, M., Rocca, A.,..., Docena, G.H. (2016) . A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders. BioFactors, 42(1), 93-105.
http://dx.doi.org/10.1002/biof.1252
---------- CHICAGO ----------
Papa Gobbi, R., De Francesco, N., Bondar, C., Muglia, C., Chirdo, F., Rumbo, M., et al. "A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders" . BioFactors 42, no. 1 (2016) : 93-105.
http://dx.doi.org/10.1002/biof.1252
---------- MLA ----------
Papa Gobbi, R., De Francesco, N., Bondar, C., Muglia, C., Chirdo, F., Rumbo, M., et al. "A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders" . BioFactors, vol. 42, no. 1, 2016, pp. 93-105.
http://dx.doi.org/10.1002/biof.1252
---------- VANCOUVER ----------
Papa Gobbi, R., De Francesco, N., Bondar, C., Muglia, C., Chirdo, F., Rumbo, M., et al. A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders. BioFactors. 2016;42(1):93-105.
http://dx.doi.org/10.1002/biof.1252