Abstract:
Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study, PESI-MS was used to monitor some biological and chemical reactions in real-time, such as acid-induced protein denaturation, hydrogen/deuterium exchange (HDX) of peptides, and Schiff base formation. By using PESI-MS, time-resolved mass spectra and ion chromatograms can be obtained reproducibly. Real-time PESI-MS monitoring can give direct and detailed information on each chemical species taking part in reactions, and this is valuable for a better understanding of the whole reaction process and for the optimization of reaction parameters. PESI-MS can be considered as a potential tool for real-time reaction monitoring due to its simplicity in instrumental setup, direct sampling with minimum sample preparation and low sample consumption. © 2010 John Wiley & Sons, Ltd.
Registro:
Documento: |
Artículo
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Título: | Real-time reaction monitoring by probe electrospray ionization mass spectrometry |
Autor: | Yu, Z.; Chen, L.C.; Erra-Balsells, R.; Nonami, H.; Hiraoka, K. |
Filiación: | Clean Energy Research Center, University of Yamanashi, Kofu, Japan CIHIDECAR-CONICET, Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, Matsuyama, Japan College of Chemistry and Biology, Shenyang Normal University, Shenyang, China
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Palabras clave: | myoglobin; peptide; article; chemical reaction; chemical structure; chemistry; computer system; electrospray mass spectrometry; evaluation; human; methodology; protein denaturation; Chemical Processes; Computer Systems; Humans; Molecular Structure; Myoglobin; Peptides; Protein Denaturation; Spectrometry, Mass, Electrospray Ionization |
Año: | 2010
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Volumen: | 24
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Número: | 11
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Página de inicio: | 1507
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Página de fin: | 1513
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DOI: |
http://dx.doi.org/10.1002/rcm.4556 |
Título revista: | Rapid Communications in Mass Spectrometry
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Título revista abreviado: | Rapid Commun. Mass Spectrom.
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ISSN: | 09514198
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CODEN: | RCMSE
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CAS: | Myoglobin; Peptides
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09514198_v24_n11_p1507_Yu |
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Citas:
---------- APA ----------
Yu, Z., Chen, L.C., Erra-Balsells, R., Nonami, H. & Hiraoka, K.
(2010)
. Real-time reaction monitoring by probe electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry, 24(11), 1507-1513.
http://dx.doi.org/10.1002/rcm.4556---------- CHICAGO ----------
Yu, Z., Chen, L.C., Erra-Balsells, R., Nonami, H., Hiraoka, K.
"Real-time reaction monitoring by probe electrospray ionization mass spectrometry"
. Rapid Communications in Mass Spectrometry 24, no. 11
(2010) : 1507-1513.
http://dx.doi.org/10.1002/rcm.4556---------- MLA ----------
Yu, Z., Chen, L.C., Erra-Balsells, R., Nonami, H., Hiraoka, K.
"Real-time reaction monitoring by probe electrospray ionization mass spectrometry"
. Rapid Communications in Mass Spectrometry, vol. 24, no. 11, 2010, pp. 1507-1513.
http://dx.doi.org/10.1002/rcm.4556---------- VANCOUVER ----------
Yu, Z., Chen, L.C., Erra-Balsells, R., Nonami, H., Hiraoka, K. Real-time reaction monitoring by probe electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom. 2010;24(11):1507-1513.
http://dx.doi.org/10.1002/rcm.4556