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Abstract:

The successful analysis by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (UV-MALDI-TOF MS) of native and hydrolyzed high-methoxylated pectin samples is described. In order to find the optimal conditions for UV-MALDI-TOF MS analysis several experimental variables were studied such as: different UV-MALDI matrices (nor-harmane, 2,5-dihydroxybenzoic acid), sample preparation methods (mixture, sandwich), inorganic salt addition (doping salts, NaCl, KCl, NH4Cl), ion mode (positive, negative), linear and reflectron mode, etc. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOF MS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. Copyright © 2007 John Wiley & Sons, Ltd.

Registro:

Documento: Artículo
Título:Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices
Autor:Monge, M.E.; Negri, R.M.; Kolender, A.A.; Erra-Balsells, R.
Filiación:Instituto de Química Física de Materiales, Ambiente Y Energía (INQUIMAE), Departamento de Química Inorgánica, Analítica Y Química Física, Pabellón 2, (C1428EHA), Buenos Aires, Argentina
CIHIDECAR-CONICET, Departamento de Química Orgánica, Pabellón 2, (C1428EHA), Buenos Aires, Argentina
Palabras clave:2,5-dihydroxybenzoic acid; drug derivative; gentisic acid; harman; harmine; pectin; unclassified drug; article; chemistry; electrospray mass spectrometry; hydroxylation; mass spectrometry; methodology; nuclear magnetic resonance spectroscopy; reproducibility; sensitivity and specificity; ultraviolet spectrophotometry; Gentisates; Harmine; Hydroxylation; Magnetic Resonance Spectroscopy; Pectins; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Ultraviolet
Año:2007
Volumen:21
Número:16
Página de inicio:2638
Página de fin:2646
DOI: http://dx.doi.org/10.1002/rcm.3121
Título revista:Rapid Communications in Mass Spectrometry
Título revista abreviado:Rapid Commun. Mass Spectrom.
ISSN:09514198
CODEN:RCMSE
CAS:gentisic acid, 490-79-9; harman, 486-84-0; harmine, 343-27-1, 442-51-3; pectin, 9000-69-5; 2,5-dihydroxybenzoic acid, 490-79-9; Gentisates; Harmine, 442-51-3; Pectins; harman, 486-84-0; pectin, 9000-69-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09514198_v21_n16_p2638_Monge

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Citas:

---------- APA ----------
Monge, M.E., Negri, R.M., Kolender, A.A. & Erra-Balsells, R. (2007) . Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices. Rapid Communications in Mass Spectrometry, 21(16), 2638-2646.
http://dx.doi.org/10.1002/rcm.3121
---------- CHICAGO ----------
Monge, M.E., Negri, R.M., Kolender, A.A., Erra-Balsells, R. "Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices" . Rapid Communications in Mass Spectrometry 21, no. 16 (2007) : 2638-2646.
http://dx.doi.org/10.1002/rcm.3121
---------- MLA ----------
Monge, M.E., Negri, R.M., Kolender, A.A., Erra-Balsells, R. "Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices" . Rapid Communications in Mass Spectrometry, vol. 21, no. 16, 2007, pp. 2638-2646.
http://dx.doi.org/10.1002/rcm.3121
---------- VANCOUVER ----------
Monge, M.E., Negri, R.M., Kolender, A.A., Erra-Balsells, R. Structural characterization of native high-methoxylated pectin using nuclear magnetic resonance spectroscopy and ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparative use of 2,5-dihydroxybenzoic acid and nor-harmane as UV-MALDI matrices. Rapid Commun. Mass Spectrom. 2007;21(16):2638-2646.
http://dx.doi.org/10.1002/rcm.3121