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Abstract:

Three xylan fractions isolated from the red seaweed Nothogenia fastigiata (Nemaliales) were analyzed by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (UV-MALDI-TOFMS). UV-MALDI-TOFMS was carried out in the linear and reflectron modes, and as routine in the positive and negative ion modes. Of the several matrices tested, nor-harmane was the only effective one giving good spectra in the positive ion mode. The number-average molar masses of two of the fractions, calculated from the distribution profiles, were lower than those determined previously by 1H NMR analysis, suggesting a decrease in the ionization efficiency with increasing molecular weight; weight-average molar mass and polydispersity index were also determined. As the xylans retained small but significant quantities of calcium salts, the influence of added Ca2+ as CaCl 2 on UV-MALDI-MS was investigated. The simultaneous addition of sodium chloride and calcium chloride was also analyzed. Addition of sodium chloride did not change the distribution profile of the native sample showing that the inhibitory effect is due to Ca2+ and not to Cl-. Addition of calcium chloride with 1:1 analyte/salt molar ratio gave spectra with less efficient desorption/ionization of oligomers; the signals of these oligomers were completely suppressed when the addition of the salt became massive (1:100 analyte/salt molar ratio). Copyright © 2005 John Wiley & Sons, Ltd.

Registro:

Documento: Artículo
Título:Matrix-assisted ultraviolet laser desorption/ionization time-of-flight mass spectrometry of β(1 → 3), β-(1 → 4)-xylans from Nothogenia fastigiata using nor-harmane as matrix
Autor:Fukuyama, Y.; Kolender, A.A.; Nishioka, M.; Nonami, H.; Matulewicz, M.C.; Erra-Balsells, R.; Cerezo, A.S.
Filiación:Plant Biophys./Biochem. Res. Lab., College of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan
Departamento de Quimica Organica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:calcium chloride; harman; sodium chloride; xylan; article; calculation; calibration; dispersion; matrix assisted laser desorption ionization time of flight mass spectrometry; molecular weight; nonhuman; Nothogenia fastigiata; polymerization; proton nuclear magnetic resonance; seaweed; ultraviolet radiation; Carbolines; Harmine; Seaweed; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Xylans
Año:2005
Volumen:19
Número:3
Página de inicio:349
Página de fin:358
DOI: http://dx.doi.org/10.1002/rcm.1783
Título revista:Rapid Communications in Mass Spectrometry
Título revista abreviado:Rapid Commun. Mass Spectrom.
ISSN:09514198
CODEN:RCMSE
CAS:calcium chloride, 10043-52-4; harman, 486-84-0; sodium chloride, 7647-14-5; xylan, 9014-63-5; Carbolines; harman, 486-84-0; Harmine, 442-51-3; Xylans
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09514198_v19_n3_p349_Fukuyama

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Citas:

---------- APA ----------
Fukuyama, Y., Kolender, A.A., Nishioka, M., Nonami, H., Matulewicz, M.C., Erra-Balsells, R. & Cerezo, A.S. (2005) . Matrix-assisted ultraviolet laser desorption/ionization time-of-flight mass spectrometry of β(1 → 3), β-(1 → 4)-xylans from Nothogenia fastigiata using nor-harmane as matrix. Rapid Communications in Mass Spectrometry, 19(3), 349-358.
http://dx.doi.org/10.1002/rcm.1783
---------- CHICAGO ----------
Fukuyama, Y., Kolender, A.A., Nishioka, M., Nonami, H., Matulewicz, M.C., Erra-Balsells, R., et al. "Matrix-assisted ultraviolet laser desorption/ionization time-of-flight mass spectrometry of β(1 → 3), β-(1 → 4)-xylans from Nothogenia fastigiata using nor-harmane as matrix" . Rapid Communications in Mass Spectrometry 19, no. 3 (2005) : 349-358.
http://dx.doi.org/10.1002/rcm.1783
---------- MLA ----------
Fukuyama, Y., Kolender, A.A., Nishioka, M., Nonami, H., Matulewicz, M.C., Erra-Balsells, R., et al. "Matrix-assisted ultraviolet laser desorption/ionization time-of-flight mass spectrometry of β(1 → 3), β-(1 → 4)-xylans from Nothogenia fastigiata using nor-harmane as matrix" . Rapid Communications in Mass Spectrometry, vol. 19, no. 3, 2005, pp. 349-358.
http://dx.doi.org/10.1002/rcm.1783
---------- VANCOUVER ----------
Fukuyama, Y., Kolender, A.A., Nishioka, M., Nonami, H., Matulewicz, M.C., Erra-Balsells, R., et al. Matrix-assisted ultraviolet laser desorption/ionization time-of-flight mass spectrometry of β(1 → 3), β-(1 → 4)-xylans from Nothogenia fastigiata using nor-harmane as matrix. Rapid Commun. Mass Spectrom. 2005;19(3):349-358.
http://dx.doi.org/10.1002/rcm.1783