Artículo

Trinchero, J.; Ponce, N.M.A.; Córdoba, O.L.; Flores, M.L.; Pampuro, S.; Stortz, C.A.; Salomón, H.; Turk, G. "Antiretroviral activity of fucoidans extracted from the brown seaweed Adenocystis utricularis" (2009) Phytotherapy Research. 23(5):707-712
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Abstract:

Treatment of human immunodeficiency virus type 1 (HIV-1, causative agent of AIDS) infection represents a major challenge in antiviral therapeutics. Many difficulties are associated with the treatment, including toxicity, resistance and high costs. Taking this into account, research for novel compounds able to overcome these limitations is needed. Sulfated polysaccharides appear to be interesting, given their abundance as components of seaweeds. Herein, a series of fractions obtained from the brown seaweed Adenocystis utricularis was analysed for in vitro anti-HIV-1 activity. These fractions, which have anti-herpes simplex virus activity, were determined previously to belong to the family of fucoidans, sulfated polysaccharides obtained from the cell walls of brown seaweeds. Assays in human PBMC primary cell culture demonstrated that two of the five fractions analysed had potent anti-HIV-1 activity both against WT and drug-resistant HIV-1 strains. For active fractions, it was also shown that the inhibitory effect was not due to an inactivating effect on the viral particle (i.e. no virucidal activity was detected) but rather to a blockade of early events of viral replication. Given these encouraging results, these seaweed-derived fractions appear as good candidates for further studies on their potential for in vivo therapy and/or prophylaxis of HIV-1 infection. Copyright © 2008 John Wiley & Sons, Ltd.

Registro:

Documento: Artículo
Título:Antiretroviral activity of fucoidans extracted from the brown seaweed Adenocystis utricularis
Autor:Trinchero, J.; Ponce, N.M.A.; Córdoba, O.L.; Flores, M.L.; Pampuro, S.; Stortz, C.A.; Salomón, H.; Turk, G.
Filiación:Centro Nacional de Referencia para el SIDA, Departamento de Microbiología, Parasitología e Inmunología, Universidad de Buenos Aires, Paraguay 2155, C1121ABG, Buenos Aires, Argentina
Farmacognosia, CRIDECIT, Universidad Nacional de la Patagonia San Juan Bosco, km 4, 9000, Comodoro Rivadavia, Chubut, Argentina
Química Biológica II, CRIDECIT, Universidad Nacional de la Patagonia San Juan Bosco, km 4, 9000, Comodoro Rivadavia, Chubut, Argentina
Departamento de Química Orgánica, CIHIDECAR, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Centro Nacional de Referencia para el SIDA, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, C1121ABG - Buenos Aires, Argentina
Palabras clave:Antiviral activity; Brown seaweeds; Fucoidans; HIV; fucoidin; galactofucan; unclassified drug; zidovudine; anti human immunodeficiency virus agent; fucoidin; plant extract; polysaccharide; adenocystis utricularis; antiviral activity; article; cell culture; cell wall; controlled study; Herpes simplex virus; human; human cell; Human immunodeficiency virus 1; Human immunodeficiency virus 1 infection; in vitro study; nonhuman; peripheral blood mononuclear cell; seaweed; virus particle; virus replication; virus resistance; brown alga; cell line; chemistry; drug effect; Human immunodeficiency virus 1; physiology; seaweed; Adenocystis utricularis; Human immunodeficiency virus 1; Simplexvirus; Algae, Brown; Anti-HIV Agents; Cell Line; Cell Wall; HIV-1; Humans; Plant Extracts; Polysaccharides; Seaweed; Virus Replication
Año:2009
Volumen:23
Número:5
Página de inicio:707
Página de fin:712
DOI: http://dx.doi.org/10.1002/ptr.2723
Título revista:Phytotherapy Research
Título revista abreviado:Phytother. Res.
ISSN:0951418X
CODEN:PHYRE
CAS:fucoidin, 9072-19-9; zidovudine, 30516-87-1; Anti-HIV Agents; Plant Extracts; Polysaccharides; fucoidan, 9072-19-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951418X_v23_n5_p707_Trinchero

Referencias:

  • Baba, M., Schols, D., De Clercq, E., Novel sulfated polymers as highly potent and selective inhibitors of human immunodeficiency virus replication and giant cell formation (1990) Antimicrob Agents Chemother, 34, pp. 134-138
  • Balzarini, J., Van Damme, L., Microbicide drug candidates to prevent HIV infection (2007) Lancet, 369, pp. 787-797
  • Beress, A., Wassermann, O., Tahhan, S., A new procedure for the isolation of anti-HIV compounds (polysaccharides and polyphenols) from the marine alga Fucus vesiculosus (1993) J Nat Prod, 56, pp. 478-488
  • Callahan, L.N., Phelan, M., Mallinson, M., Norcross, M.A., Dextran sulfate blocks antibody binding to the principal neutralizing domain of human immunodeficiency virus type 1 without interfering with gp120-CD4 interactions (1991) J Virol, 65, pp. 1543-1550
  • Chevolot, L., Foucault, A., Chaubet, F., Further data on the structure of brown seaweed fucans: Relationships with anticoagulant activity (1999) Carbohydr Res, 319, pp. 154-165
  • Cos, P., Maes, L., Vanden Berghe, D., Hermans, N., Pieters, L., Vlietinck, A., Plant substances as anti-HIV agents selected according to their putative mechanism of action (2004) J Nat Prod, 67, pp. 284-293
  • Damonte, E.B., Pujol, C.A., Coto, C.E., Prospects for the therapy and prevention of dengue virus infections (2004) Adv Virus Res, 63, pp. 239-285
  • Duarte, M.E., Cardoso, M.A., Noseda, M.D., Cerezo, A.S., Structural studies on fucoidans from the brown seaweed Sargassum stenophyllum (2001) Carbohydr Res, 333, pp. 281-293
  • Feldman, S.C., Reynaldi, S., Stortz, C.A., Cerezo, A.S., Damonte, E.B., Antiviral properties of fucoidan fractions from Leathesia difformis (1999) Phytomedicine, 6, pp. 335-340
  • Fernandez-Romero, J.A., Thorn, M., Turville, S.G., Carrageenan/MIV-150 (PC-815), a combination microbicide (2007) Sex Transm Dis, 34, pp. 9-14
  • Harrop, H.A., Coombe, D.R., Rider, C.C., Heparin specifically inhibits binding of V3 loop antibodies to HIV-1 gp120, an effect potentiated by CD4 binding (1994) AIDS, 8, pp. 183-192
  • Hart, C.A., Evans-Strickfaden, T., HIV entry inhibitors as microbicides (2007) Entry Inhibitors in HIV Therapy, pp. 99-117. , Reeves JD (ed.). Birkhauser: Basel
  • Hoffmann, C., Rockstroh, J.K., Kamps, B.D., (2006) HIV Medicine 2006, p. 818. , Flying Publisher: Paris
  • Hoshino, T., Hayashi, T., Hayashi, K., Hamada, J., Lee, J.B., Sankawa, U., An antivirally active sulfated polysaccharide from Sargassum horneri (Turner) C. Agardh (1998) Biol Pharm Bull, 21, pp. 730-734
  • Kilmarx, P.H., Van De Wijgert, J.H., Chaikummao, S., Safety and acceptability of the candidate microbicide Carraguard in Thai women: Findings from a phase II clinical trial (2006) J Acquir Immune Defic Syndr, 43, pp. 327-334
  • Kolender, A.A., Pujol, C.A., Damonte, E.B., Matulewicz, M.C., Cerezo, A.S., The system of sulfated alpha-(1->3)-linked D-mannans from the red seaweed Nothogenia fastigiata: Structures, antiherpetic and anticoagulant properties (1997) Carbohydr Res, 304, pp. 53-60
  • Moen, L.K., Clark, G.F., A novel reverse transcriptase inhibitor Fucus vesiculosus (1993) Int Conf AIDS, 9, pp. 145-161
  • Nakashima, H., Inazawa, K., Ichiyama, M., Sulfated alkyl oligosaccharides inhibit human immunodeficiency virus in vitro and provide sustained drug levels in mammals (1995) Antiviral Chem Chemother, 6, pp. 271-280
  • Nishino, T., Nagumo, T., The sulfate-content dependence of the anticoagulant activity of a fucan sulfate from the brown seaweed Ecklonia kurome (1991) Carbohydr Res, 214, pp. 193-197
  • Nishino, T., Nagumo, T., Anticoagulant and antithrombin activities of oversulfated fucans (1992) Carbohydr Res, 229, pp. 355-362
  • Nishino, T., Nagumo, T., Kiyohara, H., Yamada, H., Structural characterization of a new anticoagulant fucan sulfate from the brown seaweed Ecklonia kurome (1991) Carbohydr Res, 211, pp. 77-90
  • Nishino, T., Nishioka, C., Ura, H., Nagumo, T., Isolation and partial characterization of a novel amino sugar-containing fucan sulfate from commercial Fucus vesiculosus fucoidan (1994) Carbohydr Res, 255, pp. 213-224
  • Pauwels, R., De Clercq, E., Development of vaginal microbicides for the prevention of heterosexual transmission of HIV (1996) J Acquir Immune Defic Syndr Hum Retrovirol, 11, pp. 211-221
  • Ponce, N.M.A., (2007) Estudio del Sistema de Polisacáridos de Adenocystis utricularis, Un Alga Parda de Las Costas Patagónicas Argentinas de Posible Utilización Farmacológica Y/o Industrial, , PhD Thesis. Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, Comodoro Rivadavia
  • Ponce, N.M., Pujol, C.A., Damonte, E.B., Flores, M.L., Stortz, C.A., Fucoidans from the brown seaweed Adenocystis utricularis: Extraction methods, antiviral activity and structural studies (2003) Carbohydr Res, 338, pp. 153-165
  • Renn, D.W., Medical and biotechnological applications of marine macroalgal polysaccharides (1993) Marine Biotechnology, 1, pp. 191-196. , Attaway DH, Oskar R (eds). Plenum Press: New York
  • Schaeffer, D.J., Krylov, V.S., Anti-HIV activity of extracts and compounds from algae and cyanobacteria (2000) Ecotoxicol Environ Saf, 45, pp. 208-227
  • Venkateswaran, P.S., Millman, I., Blumberg, B.S., Interaction of fucoidan from Pelvetia fastigiata with surface antigens of hepatitis B and woodchuck hepatitis viruses (1989) Planta Med, 55, pp. 265-270
  • Whitehead, S.J., Kilmarx, P.H., Blanchard, K., Acceptability of Carraguard vaginal gel use among Thai couples (2006) AIDS, 20, pp. 2141-2148
  • Witvrouw, M., De Clercq, E., Sulfated polysaccharides extracted from sea algae as potential antiviral drugs (1997) Gen Pharmacol, 29, pp. 497-511
  • Zhuang, C., Itoh, H., Mizuno, T., Ito, H., Antitumor active fucoidan from the brown seaweed, umitoranoo (Sargassum thunbergii) (1995) Biosci Biotechnol Biochem, 59, pp. 563-567

Citas:

---------- APA ----------
Trinchero, J., Ponce, N.M.A., Córdoba, O.L., Flores, M.L., Pampuro, S., Stortz, C.A., Salomón, H.,..., Turk, G. (2009) . Antiretroviral activity of fucoidans extracted from the brown seaweed Adenocystis utricularis. Phytotherapy Research, 23(5), 707-712.
http://dx.doi.org/10.1002/ptr.2723
---------- CHICAGO ----------
Trinchero, J., Ponce, N.M.A., Córdoba, O.L., Flores, M.L., Pampuro, S., Stortz, C.A., et al. "Antiretroviral activity of fucoidans extracted from the brown seaweed Adenocystis utricularis" . Phytotherapy Research 23, no. 5 (2009) : 707-712.
http://dx.doi.org/10.1002/ptr.2723
---------- MLA ----------
Trinchero, J., Ponce, N.M.A., Córdoba, O.L., Flores, M.L., Pampuro, S., Stortz, C.A., et al. "Antiretroviral activity of fucoidans extracted from the brown seaweed Adenocystis utricularis" . Phytotherapy Research, vol. 23, no. 5, 2009, pp. 707-712.
http://dx.doi.org/10.1002/ptr.2723
---------- VANCOUVER ----------
Trinchero, J., Ponce, N.M.A., Córdoba, O.L., Flores, M.L., Pampuro, S., Stortz, C.A., et al. Antiretroviral activity of fucoidans extracted from the brown seaweed Adenocystis utricularis. Phytother. Res. 2009;23(5):707-712.
http://dx.doi.org/10.1002/ptr.2723