Artículo
Alché, L.E.; Barquero, A.A.; Sanjuan, N.A.; Coto, C.E. "An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation" (2002) Phytotherapy Research. 16(4):348-352
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Abstract:
Meliacine (MA), an antiviral principle isolated from leaves of Melia azedarach L., exhibits potent antiviral activity against herpes simplex virus type 1 (HSV-1) by inhibiting specific infected-cell polypeptides (ICPs) produced late in infection. Some of these are involved in DNA synthesis and in the assembly of nucleocapsids. The present report provides additional evidence to elucidate the mode of action of MA against HSV-1. Time-of-addition experiments confirmed that MA affects a late event in the multiplication cycle of HSV-1. We showed that MA diminished the synthesis of viral DNA and inhibited the spread of infectious viral particles when HSV-1 that expresses β-galactosidase activity was used. In addition, the lack of a protein with an apparent MW of 55 KD was detected in MA-treated cell extracts. Ultrastructural analysis of infected cells showed that, in the case of MA treatment, a large number of unenveloped nucleocapsids accumulated in the cytoplasm and a minor proportion of mature virus was found in cytoplasmic vesicles. These findings suggest that MA exerts an antiviral action on both the synthesis of viral DNA and the maturation and egress of HSV-1 during the infection of Vero cells. Copyright © 2002 John Wiley & Sons, Ltd.
Registro:
| Documento: | Artículo |
| Título: | An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation |
| Autor: | Alché, L.E.; Barquero, A.A.; Sanjuan, N.A.; Coto, C.E. |
| Filiación: | Laboratory of Virology, University of Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina Department of Microbiology, School of Medicine, University of Buenos Aires, Paraguay 2155, 1113 Buenos Aires, Argentina Lab. de Virología, Depto. de Química Biológica Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina CONICET, Argentina |
| Palabras clave: | Antiviral; Herpes simplex virus type 1; Melia azedarach L.; Meliacine; antivirus agent; beta galactosidase; meliacine; polypeptide; unclassified drug; virus DNA; animal cell; antiviral activity; aqueous solution; article; controlled study; cytoplasm; cytoplasm vesicle; DNA cleavage; DNA packaging; DNA synthesis; drug mechanism; drug synthesis; enzyme activity; Herpes simplex virus 1; medicinal plant; melia azedarach; molecular weight; nonhuman; plant leaf; time; Vero cell; virus envelope; virus inhibition; virus nucleocapsid; virus particle; virus replication; Animals; Antiviral Agents; Cell Transformation, Viral; Cercopithecus aethiops; DNA, Viral; Herpesvirus 1, Human; Meliaceae; Plant Extracts; Plant Leaves; Vero Cells; Viral Proteins; Virus Replication; Human herpesvirus 1; Melia azedarach; Simplexvirus |
| Año: | 2002 |
| Volumen: | 16 |
| Número: | 4 |
| Página de inicio: | 348 |
| Página de fin: | 352 |
| DOI: | http://dx.doi.org/10.1002/ptr.895 |
| Título revista: | Phytotherapy Research |
| Título revista abreviado: | Phytother. Res. |
| ISSN: | 0951418X |
| CODEN: | PHYRE |
| CAS: | Antiviral Agents; DNA, Viral; Plant Extracts; Viral Proteins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951418X_v16_n4_p348_Alche |
Referencias:
- Alché, L.E., Berra, A., Veloso, M.J., Coto, C.E., Treatment with meliacine, a plant derived antiviral, inhibits herpetic stromal queratitis in mice (2000) J Med Virol, 61 (4), pp. 474-480
- Alrabiah, F.A., Sacks, S.L., New antiherpes virus agents: Their targets and therapeutic potential (1996) Drugs, 52, pp. 17-32
- Andrei, G., Couto, A.S., Lederkremer, R.M., Coto, C.E., Purification and partial characterization of an antiviral active peptide from Melia azedarach L (1994) Antiviral Chem Chemother, 5, pp. 105-110
- Barquero, A.A., Alché, L.E., Coto, C.E., Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of herpes simplex virus type 1 alone and in combination with acyclovir (1997) Int J Antimicrob Agents, 9, pp. 49-55
- Benencia, F., Courreges, M.C., Coulombié, F.C., Antiviral activity of crude polysaccharides from Trichilia glabra leaves (1997) Fitoterapia, 68, pp. 173-175
- Campadelli-Fiume, G., Farabegoli, F., Gaeta, S., Roizman, B., Origin of unenveloped capsids in the cytoplasm of cells infected with herpes simplex virus 1 (1991) J Virol, 65, pp. 1589-1595
- Cheung, P., Banfield, B.W., Tufaro, F., Brefeldin A arrests the maturation and egress of herpes simplex virus particles during infection (1991) J Virol, 65, pp. 1893-1904
- Cordell, G.A., Changing strategies in natural products chemistry (1995) Phytochemistry, 40, pp. 1585-1612
- Córdoba, M.A., Coto, C.E., Damonte, E.B., Virucidal activity in aqueous extracts obtained from Cedrela tubiflora leaves (1991) Phytother Res, 5, pp. 250-253
- Darby, G., In search of the perfect antiviral (1995) Antiviral Chem Chemother, 6, pp. 54-63
- Hirsch, M.S., Kaplan, J.C., D'Aquila, R.T., Antiviral agents (1996) Virology, 3rd edn, pp. 436-445. , Fields BN, Knipe DM, Howley PM (eds). Lippincott-Raven: Philadelphia
- Johnson, P.A., Best, M.G., Friedmann, T., Parris, D.S., Isolation of a herpes simplex virus type 1 mutant deleted for the essential UL42 gene and characterization of its null phenotype (1991) J Virol, 65, pp. 700-710
- Koyama, A.H., Uchida, T., Quantitative studies on the maturation process of herpes simplex virus type 1 in Vero cells (1988) Virus Res, 10, pp. 281-285
- Mahmood, N., PIacente, S., Burke, A., Khan, A., Pizza, C., Constituents of Cuscuta reflexa are anti-HIV agents (1997) Antiviral Chem Chemother, 8, pp. 70-74
- Ngan, F., Chang, R.S., Tabba, H.D., Smith, K.M., Isolation, purification and partial characterization of an active anti-HIV compound from the Chinese medicinal herb Viola yedoensis (1988) Antiviral Res, 10, pp. 107-116
- Prusoff, W.H., Lin, T.-S., August, E.M., Overview of molecular aspects of antiviral drug action and design (1990) Pharmacol Ther, (SUPPL.), pp. 11-24. , Chapter 2
- Souza Brito, A.R.M., How to study the pharmacology of medicinal plants in underdeveloped countries (1996) J Ethnopharmacol, 54, pp. 131-138
- Villamil, S.M., Alché, L.E., Coto, C.E., Inhibition of herpes simplex virus type 1 multiplication by meliacine, a peptide of plant origin (1995) Antiviral Chem Chemother, 6, pp. 239-244
- Wachsman, M.B., Martino, V., Coto, C.E., Search for antiviral activity in higher plant extracts (1988) Fitoterapia, 59, pp. 422-424
- Waxman, L., Darke, P., The herpesvirus proteases as targets for antiviral chemotherapy (2000) Antiviral Chem Chemother, 11, pp. 1-22
- Xu Ze-Qi, Kern, E.R., Westbrook, L., Plant-derived and semi-synthetic calanolide compounds with in vitro activity against both human immunodeficiency virus type 1 and human cytomegalovirus (2000) Antiviral Chem Chemother, 11, pp. 23-29
Citas:
---------- APA ----------
Alché, L.E., Barquero, A.A., Sanjuan, N.A. & Coto, C.E. (2002) . An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation. Phytotherapy Research, 16(4), 348-352.http://dx.doi.org/10.1002/ptr.895
---------- CHICAGO ----------
Alché, L.E., Barquero, A.A., Sanjuan, N.A., Coto, C.E. "An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation" . Phytotherapy Research 16, no. 4 (2002) : 348-352.http://dx.doi.org/10.1002/ptr.895
---------- MLA ----------
Alché, L.E., Barquero, A.A., Sanjuan, N.A., Coto, C.E. "An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation" . Phytotherapy Research, vol. 16, no. 4, 2002, pp. 348-352.http://dx.doi.org/10.1002/ptr.895
---------- VANCOUVER ----------
Alché, L.E., Barquero, A.A., Sanjuan, N.A., Coto, C.E. An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation. Phytother. Res. 2002;16(4):348-352.http://dx.doi.org/10.1002/ptr.895
