Abstract:
A new one-step method for protein measurement based in the copper-neocuproine complex formation is described. A linear response was obtained for different proteins in the range of 0.5 to 40 μg/ml with a sensitivity of 0.5 μg protein/ml. © 1991 Science & Technology Letters.
Registro:
Documento: |
Artículo
|
Título: | Protein measurement with neocuproine reactive |
Autor: | Castro, G.R.; Méndez, B.S.; Siñeriz, F. |
Filiación: | PROIMI - MIRCEN. Avda. Belgrano y Pje. Caseros, Tucumán, 4000, Argentina Depto. Qca. Biológica, Facultad de Ciencias Exactas y Naturales, U. de Buenos Aires, Buenos Aires, 1428, Argentina
|
Año: | 1991
|
Volumen: | 5
|
Número: | 6
|
Página de inicio: | 431
|
Página de fin: | 436
|
DOI: |
http://dx.doi.org/10.1007/BF00155488 |
Título revista: | Biotechnology Techniques
|
Título revista abreviado: | Biotechnol Tech
|
ISSN: | 0951208X
|
CODEN: | BTECE
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951208X_v5_n6_p431_Castro |
Referencias:
- Bradford, M.M., (1976) Anal. Biochem., 72, pp. 248-254
- Dygert, S., Li, L.H., Florida, D., Thoma, J.A., (1965) Anal. Biochem., 13, pp. 367-374
- Kirschenbaum, D.M., (1970) Handbook of Biochemistry, pp. 71-98. , H., Sober, Chemical Rubber Co., Cleveland
- Lowry, O.H., Rosembrough, N.J., Lewis Farr, A., Randall, R.J., (1951) J. Biol. Chem., 193, pp. 265-275
- Mc Guire, J., Taylor, P., Greene, L.A., (1977) Anal. Biochem., 83, pp. 75-81
- Pierce, J., Suelter, C.H., (1977) Anal. Biochem., 81, pp. 478-480
- Sedmak, J.J., Grossberg, S.E., (1977) Anal. Biochem., 79, pp. 544-552
- Smith, G.F., McCurdy, W.H., (1952) Anal. Chem., 24, pp. 371-373
- Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Klenk, D.C., (1985) Anal. Biochem., 150, pp. 76-85
- Van Kley, H., Hale, S.M., (1977) Anal. Biochem., 81, pp. 485-487
Citas:
---------- APA ----------
Castro, G.R., Méndez, B.S. & Siñeriz, F.
(1991)
. Protein measurement with neocuproine reactive. Biotechnology Techniques, 5(6), 431-436.
http://dx.doi.org/10.1007/BF00155488---------- CHICAGO ----------
Castro, G.R., Méndez, B.S., Siñeriz, F.
"Protein measurement with neocuproine reactive"
. Biotechnology Techniques 5, no. 6
(1991) : 431-436.
http://dx.doi.org/10.1007/BF00155488---------- MLA ----------
Castro, G.R., Méndez, B.S., Siñeriz, F.
"Protein measurement with neocuproine reactive"
. Biotechnology Techniques, vol. 5, no. 6, 1991, pp. 431-436.
http://dx.doi.org/10.1007/BF00155488---------- VANCOUVER ----------
Castro, G.R., Méndez, B.S., Siñeriz, F. Protein measurement with neocuproine reactive. Biotechnol Tech. 1991;5(6):431-436.
http://dx.doi.org/10.1007/BF00155488