Abstract:
A rapid method was developed for plasmid DNA screening from micro volume cultures of Clostridium acetobutylicum. It involves protoplastization by lysozyme, nuclease inhibition by incorporating EDTA or DEP, followed by lysis with high concentration of SDS. The whole lysate is applied directly to electrophoretic analysis. © 1987 Chapman & Hall.
Referencias:
- Benassi, F.O., Bloos, R.K., de Rambaldo, L.A., (1983) Rev. Arg. de Microbiol., 15, pp. 65-71
- Birnboim, H.C., Doly, J., (1979) Nucleic. Acids. Res., 7, pp. 1513-1523
- Blaschek, H.P., Klacik, M.A., (1984) J.Bacteriol., 147, pp. 262-266
- Davis, R.W., Botstein, D., Roth, J.R., (1980) advanced Bacterial Genetics Laboratory Manual, p. 201. , Cold Spring Harbor Laboratory, New York
- Eckhardt, T., (1978) Plasmid, 1, pp. 584-588
- Floccari, M.E., Schijman, A.G., Méndez, B.S., (1986) Abstracts of the IV Southern Cone Meeting of the Panamerican Association of Biochemical Societies Nov26–29, , Pucon, Chile
- Guerry, P., Le Blanc, D.J., Falkow, S., (1973) J. Bacteriol., 116, pp. 1064-1066
- Herrero, A.H., Gómez, R.F., (1980) Appl. Environ Microbiol., 40, pp. 571-577
- Jones, D.T., Jones, W.A., Woods, D.R., (1985) J.Gen. Microibol., 131, pp. 1213-1216
- Lin, Y.L., Blaschek, H.P., (1984) Appl. Environ. Microbiol., 48, pp. 737-742
- Maniatis, T., Fritsch, E.F., Sambrook, J., (1982) Molecular cloning, p. 160. , Cold Spring Harbor Laboratory, Cold Spring Harbor. New York
- Meyers, J.A., Sánchez, D., Elwell, L.B., Falkow, S., (1976) J. Bacteriol., 127, pp. 1529-1537
- O'Brien, R.W., Morris, J.A., (1971) J. Gen. Microbiol., 68, pp. 307-318
- Oultram, J., Young, M., (1985) FEMS. Microbiol. Lett., 27, pp. 129-134
- Reysset, G., Sebald, M., (1985) Ann. Inst. Pasteur Microbiol., 136, pp. 275-282
- Schnaitman, C.A., (1971) J. Bacteriol., 108, pp. 553-563
- Strom, M.S., Eklund, Poysky, F., (1984) Appl.Env.Microbiol., 48, pp. 956-963
- Truffaut, N., Sebald, M., (1983) Mol.Gen. Genet., 189, pp. 178-180
- Urano, N., Karube, I., Suzoki, S., Yamada, T., (1983) Eur. J. Appl. Microbiol. Biotechnol., 17, pp. 349-354
- Youngman, P.J., Perkins, B.J., Losik, R., (1983) Proc. Natl. Acad.Sci. USA., 80, pp. 2305-2309
- Yu, P., Pearce, L.E., (1986) Biotechnol. Lett., 8, pp. 469-474
Citas:
---------- APA ----------
Schijman, A.G., Floccari, M.E. & Méndez, B.S.
(1987)
. A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp. Biotechnology Techniques, 1(3), 163-168.
http://dx.doi.org/10.1007/BF00227554---------- CHICAGO ----------
Schijman, A.G., Floccari, M.E., Méndez, B.S.
"A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp."
Biotechnology Techniques 1, no. 3
(1987) : 163-168.
http://dx.doi.org/10.1007/BF00227554---------- MLA ----------
Schijman, A.G., Floccari, M.E., Méndez, B.S.
"A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp."
Biotechnology Techniques, vol. 1, no. 3, 1987, pp. 163-168.
http://dx.doi.org/10.1007/BF00227554---------- VANCOUVER ----------
Schijman, A.G., Floccari, M.E., Méndez, B.S. A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp. Biotechnol Tech. 1987;1(3):163-168.
http://dx.doi.org/10.1007/BF00227554