Artículo
Terebiznik, M.R.; Zylberman, V.; Buera, M.P.; Pilosof, A.M.R. "Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose" (1998) Biotechnology Techniques. 12(9):683-687
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Abstract:
Thermal resistance of freeze-dried α-amylase and α-glucosidase in trehalose matrices (1 to 20 % w/v) stored at 90 °C and relative humidities (RH) between 0 and 44 % was studied. At RH values up to 33 %, 10 % (w/v) trehalose was necessary to retain at least 50 % of α-amylase activity. For α-glucosidase, 10 % (w/v) trehalose was effective only at 0 % RH. Ultrafiltration of the crude enzymatic fermentation extracts enhanced enzyme stability per se. However, ultrafiltration in combination with 1% (w/v) trehalose retained 74 % of α-glucosidase and 95 % of α-amylase activities.
Registro:
| Documento: | Artículo |
| Título: | Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose |
| Autor: | Terebiznik, M.R.; Zylberman, V.; Buera, M.P.; Pilosof, A.M.R. |
| Filiación: | Dto. de Industrias, FCEyN, C. Universitaria, (1428) Buenos Aires, Argentina Dto. de Ingeniería Química, Fl. Universidad de Buenos Aires, C. Universitaria, (1428) Buenos Aires, Argentina |
| Palabras clave: | alpha glucosidase; amylase; enzyme stability; extractive fermentation; freeze drying; heat resistance; humidity; microbe metabolism; trehalose; ultrafiltration; Aspergillus oryzae |
| Año: | 1998 |
| Volumen: | 12 |
| Número: | 9 |
| Página de inicio: | 683 |
| Página de fin: | 687 |
| DOI: | http://dx.doi.org/10.1023/A:1008860819947 |
| Título revista: | Biotechnology Techniques |
| Título revista abreviado: | Biotechnol. Tech. |
| ISSN: | 0951208X |
| CODEN: | BTECE |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951208X_v12_n9_p683_Terebiznik |
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Citas:
---------- APA ----------
Terebiznik, M.R., Zylberman, V., Buera, M.P. & Pilosof, A.M.R. (1998) . Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose. Biotechnology Techniques, 12(9), 683-687.http://dx.doi.org/10.1023/A:1008860819947
---------- CHICAGO ----------
Terebiznik, M.R., Zylberman, V., Buera, M.P., Pilosof, A.M.R. "Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose" . Biotechnology Techniques 12, no. 9 (1998) : 683-687.http://dx.doi.org/10.1023/A:1008860819947
---------- MLA ----------
Terebiznik, M.R., Zylberman, V., Buera, M.P., Pilosof, A.M.R. "Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose" . Biotechnology Techniques, vol. 12, no. 9, 1998, pp. 683-687.http://dx.doi.org/10.1023/A:1008860819947
---------- VANCOUVER ----------
Terebiznik, M.R., Zylberman, V., Buera, M.P., Pilosof, A.M.R. Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose. Biotechnol. Tech. 1998;12(9):683-687.http://dx.doi.org/10.1023/A:1008860819947
