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Salatino, M.; Beguelin, W.; Peters, M.G.; Carnevale, R.; Proietti, C.J.; Galigniana, M.D.; Vedoy, C.G.; Schillaci, R.; Charreau, E.H.; Sogayar, M.C.; Elizalde, P.V. "Progestin-induced caveolin-1 expression mediates breast cancer cell proliferation" (2006) Oncogene. 25(59):7723-7739
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Abstract:

Progestin regulation of gene expression was assessed in the progestin-dependent murine tumor line C4HD which requires MPA, a synthetic progestin, for in vivo growth and expresses high levels of progesterone receptor (PR). By using suppressive subtractive hybridization, caveolin-1 was identified as a gene whose expression was increased with in vivo MPA treatment. By Northern and Western blot analysis, we further confirmed that caveolin-1 mRNA and protein expression increased in MPA-treated tumors as compared with untreated tumors. When primary cultures of C4HD cells were treated in vitro with MPA, caveolin-1 levels also increased, effect that was abolished by pre-treatment with progestin antagonist RU486. In addition, MPA promoted strong caveolin-1 promoter transcriptional activation both in mouse and human breast cancer cells. We also showed that MPA regulation of caveolin-1 expression involved in activation of two signaling pathways: MAPK and PI-3K. Short-term MPA treatment of C4HD cells led to tyrosine phosphorylation of caveolin-1 protein, where Src was the kinase involved. Additionally, we showed that MPA-induced association of caveolin-1 and PR, which was detected by coimmunoprecipitation and by confocal microscopy. Finally, we proved that MPA-induced proliferation of C4HD cells was inhibited by suppression of caveolin-1 expression with antisense oligodeoxynucleotides to caveolin-1 mRNA. Furthermore, we observed that inhibition of caveolin-1 expression abrogated PR capacity to induced luciferase activity from a progesterone response element-driven reporter plasmid. Comprehensively, our results demonstrated for the first time that caveolin-1 expression is upregulated by progestin in breast cancer. We also demonstrated that caveolin-1 is a downstream effector of MPA that is partially responsible for the stimulation of growth of breast cancer cells. © 2006 Nature Publishing Group All rights reserved.

Registro:

Documento: Artículo
Título:Progestin-induced caveolin-1 expression mediates breast cancer cell proliferation
Autor:Salatino, M.; Beguelin, W.; Peters, M.G.; Carnevale, R.; Proietti, C.J.; Galigniana, M.D.; Vedoy, C.G.; Schillaci, R.; Charreau, E.H.; Sogayar, M.C.; Elizalde, P.V.
Filiación:Laboratory of Molecular Mechanisms of Carcinogenesis, Instituto de Biología Y Medicina Experimental, Consejo Nacional de Investigaciones Científicas Y Técnicas, Buenos Aires, Argentina
Instituto de Oncología Angel H. Roffo, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto Leloir, Consejo Nacional de Investigaciones Científicas Y Técnicas, Buenos Aires, Argentina
Instituto Da Química, Universidad de Sao Paulo, Sao Paoulo, Brazil
Laboratory of Molecular Mechanisms of Carcinogenesis, Instituto de Biología Y Medicina Experimental (IBYME), Obligado 2490, Buenos Aires 1428, Argentina
Palabras clave:Breast cancer; Caveolin-1; Progesterone receptor; antisense oligodeoxynucleotide; caveolin 1; gestagen; medroxyprogesterone acetate; messenger RNA; mifepristone; mitogen activated protein kinase; phosphatidylinositol 3 kinase; progesterone receptor; tyrosine; animal cell; animal experiment; article; breast cancer; cancer cell; cell activation; cell culture; cell growth; cell proliferation; cell stimulation; confocal microscopy; controlled study; DNA responsive element; female; gene expression; human; human cell; immunoprecipitation; in vitro study; mouse; nonhuman; Northern blotting; oncogene src; priority journal; protein expression; protein phosphorylation; receptor upregulation; suppression subtractive hybridization; Western blotting; 1-Phosphatidylinositol 3-Kinase; Amino Acid Sequence; Animals; Base Sequence; Caveolin 1; Female; Gene Expression Regulation, Neoplastic; Mammary Neoplasms, Experimental; MAP Kinase Signaling System; Medroxyprogesterone 17-Acetate; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Phosphorylation; Promoter Regions (Genetics); Receptors, Progesterone; src-Family Kinases; Murinae
Año:2006
Volumen:25
Número:59
Página de inicio:7723
Página de fin:7739
DOI: http://dx.doi.org/10.1038/sj.onc.1209757
Título revista:Oncogene
Título revista abreviado:Oncogene
ISSN:09509232
CODEN:ONCNE
CAS:medroxyprogesterone acetate, 71-58-9; mifepristone, 84371-65-3; mitogen activated protein kinase, 142243-02-5; phosphatidylinositol 3 kinase, 115926-52-8; tyrosine, 16870-43-2, 55520-40-6, 60-18-4; 1-Phosphatidylinositol 3-Kinase, EC 2.7.1.137; Caveolin 1; Medroxyprogesterone 17-Acetate, 71-58-9; Receptors, Progesterone; src-Family Kinases, EC 2.7.1.112
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09509232_v25_n59_p7723_Salatino

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Citas:

---------- APA ----------
Salatino, M., Beguelin, W., Peters, M.G., Carnevale, R., Proietti, C.J., Galigniana, M.D., Vedoy, C.G.,..., Elizalde, P.V. (2006) . Progestin-induced caveolin-1 expression mediates breast cancer cell proliferation. Oncogene, 25(59), 7723-7739.
http://dx.doi.org/10.1038/sj.onc.1209757
---------- CHICAGO ----------
Salatino, M., Beguelin, W., Peters, M.G., Carnevale, R., Proietti, C.J., Galigniana, M.D., et al. "Progestin-induced caveolin-1 expression mediates breast cancer cell proliferation" . Oncogene 25, no. 59 (2006) : 7723-7739.
http://dx.doi.org/10.1038/sj.onc.1209757
---------- MLA ----------
Salatino, M., Beguelin, W., Peters, M.G., Carnevale, R., Proietti, C.J., Galigniana, M.D., et al. "Progestin-induced caveolin-1 expression mediates breast cancer cell proliferation" . Oncogene, vol. 25, no. 59, 2006, pp. 7723-7739.
http://dx.doi.org/10.1038/sj.onc.1209757
---------- VANCOUVER ----------
Salatino, M., Beguelin, W., Peters, M.G., Carnevale, R., Proietti, C.J., Galigniana, M.D., et al. Progestin-induced caveolin-1 expression mediates breast cancer cell proliferation. Oncogene. 2006;25(59):7723-7739.
http://dx.doi.org/10.1038/sj.onc.1209757